数字农科院2.0

Improved detection sensitivity of anti-PRV variant antibodies through preparation of anti-gB and anti-gE monoclonal antibodies and development of blocking ELISAs

文献类型： 外文期刊

作者： Zhenyang Guo;Hu Xu;Siyu Zhang;Haonan Kang;Chao Li;Qi Sun;Jing Zhao;Jinhao Li;Guohui Zhou;Qian Wang;Lirun Xiang;Yandong Tang;Huairan Liu;Chaoliang Leng;Tongqing An;Xuehui Cai;Zhijun Tian;Hongliang Zhang;Jinmei Peng

作者机构：

关键词： Blocking ELISA;Monoclonal antibody and B-cell epitope;Pseudorabies virus

期刊名称： International Journal of Biological Macromolecules

ISSN： 0141-8130

年卷期： 2024 年 260 卷

页码：

收录情况： SCIE（2024版） ; ; EI（2024版）

摘要： Since 2011, PRV has resurged in China and is characterized by a mutated strain with significant alterations in antigenicity and virulence. Therefore, we hypothesized that antibody detection kits based on classic PRV strains may have limitations in detecting PRV variants. For more sensitive antibody detection of PRV variants, two MABs targeting the gB and gE proteins were developed. IFA revealed that these MABs exhibited strong reactivity toward both classic and variant PRV strains. MAB-gE recognizes a novel conserved linear B-cell epitope (41PSAEVWD47), while MAB-gB recognizes a conformational B-cell epitope. The binding of both MABs was effectively inhibited in the PRV-positive pig blood samples. Accordingly, we established blocking-ELISAs to detect anti-PRV gB and gE antibodies, which achieved higher sensitivity than commercial kits. Moreover, the clinical serum samples results of our method and that of IFA were in high agreement, and our test results had a higher coincidence rate than that of a commercial kit. Assessing antibody levels by our methods at various times following immunization and challenge accurately reflected the trend of antibody-level changes and revealed the conversion to positive antibody status before the commercial kit. Our method is crucial for monitoring PRV infections, assessing immune responses, and controlling disease.

分类号：

  • 相关文献

[1]Development of blocking ELISA for detection of antibodies against H9N2 avian influenza viruses. Li, Zejun.

[2]Development of a blocking ELISA for detection of antibodies against avian hepatitis E virus. Liu, Baoyuan,Zhao, Qin,Sun, Yani,Wang, Xinjie,Zhao, Jinan,Du, Taofeng,Wang, Chengbao,Xiao, Shuqi,Mu, Yang,Zhang, Gaiping,Zhou, En-Min,Liu, Baoyuan,Zhao, Qin,Sun, Yani,Wang, Xinjie,Zhao, Jinan,Du, Taofeng,Wang, Chengbao,Xiao, Shuqi,Mu, Yang,Zhou, En-Min,Zhang, Gaiping,Luo, Jianxuri,Hsu, Walter H..

[3]Development of a blocking ELISA for detection of serum neutralizing antibodies against porcine circovirus type 2. Huang, Liping,Lu, Yuehua,Wei, Yanwu,Guo, Longjun,Liu, Changming. 2011

[4]Preparation of Monoclonal Antibodies against the Viral p54 Protein and a Blocking ELISA for Detection of the Antibody against African Swine Fever Virus. Gao Y.,Xia T.,Bai J.,Zhang L.,Zheng H.,Jiang P.. 2022

[5]Establishment and application of a solid-phase blocking ELISA method for detection of antibodies against classical swine fever virus. Yuying Cao,Li Yuan,Shunli Yang,Youjun Shang,Bin Yang,Zhizhong Jing,Huichen Guo,Shuanghui Yin. 2022

[6]Development and evaluation of a blocking ELISA for serological diagnosis of equine infectious anemia. Hu, Zhe,Guo, Kui,Du, Cheng,Sun, Jinhui,Naletoski, Ivancho,Chu, Xiaoyu,Lin, Yuezhi,Wang, Xuefeng,Barrandeguy, Maria,Samuel, Mino,Wang, Wen,Lau, Patrick Imtung,Wernery, Ulrich,Raghavan, Rekha,Wang, Xiaojun. 2023

[7]A Customized Novel Blocking ELISA for Detection of Bat-Origin Swine Acute Diarrhea Syndrome Coronavirus Infection. Cao, Liyan,Kong, Xiangyu,Li, Xiangtong,Suo, Xuepeng,Duan, Yueyue,Yuan, Cong,Zhang, Yu,Zheng, Haixue,Wang, Qi. 2023

[8]Novel P22-monoclonal antibody based blocking ELISA for the detection of African swine fever virus antibodies in serum. Tsegay G.,Tesfagaber W.,Zhu Y.,He X.,Wang W.,Zhang Z.,Sun E.,Zhang J.,Guan Y.,Li F.,Liu R.,Bu Z.,Zhao D.. 2022

[9]A highly efficient blocking ELISA based on p72 monoclonal antibody for the detection of African swine fever virus antibodies and identification of its linear B cell epitope. Weldu Tesfagaber,Wan Wang,Lulu Wang,Rui Zhao,Yuanmao Zhu,Fang Li,Encheng Sun,Renqiang Liu,Zhigao Bu,Geng Meng,Dongming Zhao. 2024

[10]Development and application of a blocking ELISA based on a N protein monoclonal antibody for the antibody detection against porcine reproductive and respiratory syndrome virus 2. Wansheng Li,Yanwei Li,Minhua Li,Hongliang Zhang,Zixuan Feng,Hu Xu,Chao Li,Zhenyang Guo,Bangjun Gong,Jinmei Peng,Guohui Zhou,Zhijun Tian,Qian Wang. 2024

[11]Development and application of a blocking ELISA method based on Cap protein for detecting antibodies against porcine circovirus 2. Liu, Qingyan,Gao, Shuolei,Li, Jiannan,Yang, Jingyi,Zhu, Yuxiang,Zhu, Jun,Zhou, Yanjun,Shan, Tongling,Tong, Wu,Zheng, Hao,Kong, Ning,Jiang, Yifeng,Liu, Changlong,Tong, Guangzhi,Yu, Hai. 2025

[12]Development of a blocking ELISA employing a VP1-specific monoclonal antibody for the detection of DHAV3 antibodies. Cuiping Song,Tianyuan Li,Jing Wang,Peidong Guo,Wenjing Yang,Ning Tang,Yang Qu,Siyu Li,Xusheng Qiu,Lei Tan,Yingjie Sun,Ying Liao,Chan Ding. 2025

[13]Development of a Blocking ELISA for Detection of Serum Neutralizing Antibodies Against Duck Adenovirus Type 3. Mei Tang,Xiaona Shi,Yifei Xiong,Chunxiu Yuan,Qinmin Zhu,Minfan Huang,Bangfeng Xu,Qinfang Liu,Xue Pan,Zhifei Zhang,Qiaoyang Teng,Minghao Yan,Dawei Yan,Zejun Li. 2025

[14]Sensitive detection of HEV antibodies using a blocking ELISA based on ORF2-specific monoclonal antibodies. wanghaojie,,wuyang,,liuxing,,shanbowen,,xuelihong,,sunyue,,antongqing,,lichangwen,,chenhongyan,,yinxin,,yuchangqing,,xiachangyou,,zhanghe. 2025

[15]Highly Efficient CRISPR/Cas9-Mediated Homologous Recombination Promotes the Rapid Generation of Bacterial Artificial Chromosomes of Pseudorabies Virus. Guo, Jin-Chao,Tang, Yan-Dong,Zhao, Kuan,Wang, Tong-Yun,Liu, Ji-Ting,Gao, Jia-Cong,Chang, Xiao-Bo,Cui, Hong-Yu,Tian, Zhi-Jun,Can, Xue-Hui,An, Tong-Qing. 2016

[16]Emergence of a Pseudorabies virus variant with increased virulence to piglets. Tong, Wu,Liu, Fei,Zheng, Hao,Liang, Chao,Zhou, Yan-jun,Jiang, Yi-feng,Shan, Tong-ling,Gao, Fei,Li, Guo-xin,Tong, Guang-zhi,Tong, Wu,Zheng, Hao,Li, Guo-xin,Tong, Guang-zhi.

[17]Recombinant Pseudorabies Virus (PRV) Expressing Firefly Luciferase Effectively Screened for CRISPR/Cas9 Single Guide RNAs and Antiviral Compounds. Tang, Yan-Dong,Liu, Ji-Ting,Fang, Qiong-Qiong,Wang, Tong-Yun,Sun, Ming-Xia,An, Tong-Qing,Tian, Zhi-Jun,Cai, Xue-Hui,Liu, Ji-Ting. 2016

[18]A live, attenuated pseudorabies virus strain JS-2012 deleted for gE/gI protects against both classical and emerging strains. Tong, Wu,Li, Guoxin,Liang, Chao,Liu, Fei,Tian, Qing,Cao, Yanyun,Li, Lin,Zheng, Xuchen,Zheng, Hao,Tong, Guangzhi,Tong, Wu,Li, Guoxin,Tong, Guangzhi.

[19]Genomic analyses reveal that partial sequence of an earlier pseudorabies virus in China is originated from a Bartha-vaccine-like strain. Ye, Chao,Guo, Jin-Chao,Gao, Jia-Cong,Wang, Tong-Yun,Zhao, Kuan,Chang, Xiao-Bo,Wang, Qian,Peng, Jin-Mei,Tian, Zhi-Jun,Cai, Xue-Hui,An, Tong-Qing,Ye, Chao,Tong, Guang-Zhi.

[20]Expression of bioactive porcine interferon-alpha in Lactobacillus casei. Ma, Shi-jie,Li, Xin-Sheng,Guo, Xiao-Qing,Fu, Peng-Fei,Yang, Ming-Fan,Chen, Hong-Ying,Li, Kun.

作者其他论文 更多>>