数字农科院2.0

Rapid Russula senecis identification assays using loop-mediated isothermal amplification based on real-time fluorescence and visualization

文献类型： 外文期刊

作者： Wang, Nan;Gao, Jie;Tian, Enjing;Yu, Wenjie;Li, Hui;Zhang, Juan;Xie, Ruibin;Chen, Ailiang

作者机构：

关键词： Poisonous mushroom;Species identification;Loop-mediated isothermal amplification;Russula senecis;Visualization

期刊名称： APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

ISSN： 0175-7598

年卷期： 2022 年 106 卷

页码：

收录情况： SCIE（2022版） ; EI（2022版）

摘要： Russula senecis, a common poisonous mushroom, is widely distributed in China. Mushroom poisoning is becoming a major threat to human health and its rate is increasing worldwide. For the first time, we developed a set of loop-mediated isothermal amplification (LAMP) assays based on a real-time fluorescence and a visualization method to detect R. senecis, and the visual LAMP reaction system was optimized to further shorten the reaction time. Both real-time LAMP and visual LAMP could detect as low as 3.2 pg of genomic DNA. In addition, fried and digested mushrooms were used to validate the proposed LAMP method, and mushroom mixtures with as low as 1% of the target species could be successfully detected, indicating that the LAMP assays established in this study had good applicability and could be used for clinical sample detection and forensic identification. Furthermore, the LAMP assays were proven to be comparable to the real-time PCR method.

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