数字农科院2.0

Aqueous two-phase system based on pH-responsive polymeric deep eutectic solvent for efficient extraction of aromatic amino acids

文献类型： 外文期刊

作者： Cai, Changyong;Ma, Shaoping;Li, Fenfang;Tan, Zhijian

作者机构：

关键词： Polymeric deep eutectic solvents;pH -responsive characteristic;Aqueous two-phase systems;Aromatic amino acids

期刊名称： FOOD CHEMISTRY

ISSN： 0308-8146

年卷期： 2024 年 430 卷

页码：

收录情况： SCIE（2024版） ; ; EI（2024版）

摘要： Recently, more and more attention has been paid to the construction of stimulus-responsive aqueous two-phase systems (ATPSs) for the extraction and separation of various bioactive compounds. In this work, an ATPS based on a pH-responsive polymeric deep eutectic solvent (PDES) and phosphate salt was constructed for the first time. The pH-response properties of the PDES were studied through a series of experiments. Additionally, the phase formation mechanism was studied through experiments and simulations. This novel PDES-based ATPS was used to extract aromatic amino acids (AAAs). The extraction efficiencies for tyrosine (Tyr), phenylalanine (Phe), and tryptophan (Trp) reached 95.25%, 99.05%, and 99.10%, respectively. By adjusting pH, PDES was recycled and reused. This novel and recyclable PDES-based ATPS could be an efficient method for the extraction of AAAs, which could also be applied used as a versatile and sustainable method for the extraction of other bioactive compounds in the future.

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