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Enhanced lactic acid production through enzymatic hydrolysis: Assessing impact of varied enzyme loadings on co-fermentation of swine manure and apple waste

文献类型： 外文期刊

作者： Tianjing Lian;Fubin Yin;Wanqin Zhang;Qitao Cao;Shunli Wang;Tanlong Zhou;Fangyu Zhang;Ronghua Li;Hongmin Dong

作者机构：

关键词： Agricultural wastes;Anaerobic co-fermentation;Bacterial community analysis;Enzymatic pretreatment;Lactate

期刊名称： Bioresource Technology

ISSN： 0960-8524

年卷期： 2024 年 406 卷

页码：

收录情况： SCIE（2024版） ; ; EI（2024版）

摘要： Anaerobic co-fermentation of swine manure (SM) and apple waste (AW) restricts by the slow hydrolysis of substrates with complex structures, which subsequently leads to low lactic acid (LA) production. Therefore, a novel strategy based on enzymatic pretreatment for improving LA production from anaerobic co-fermentation of SM and AW was proposed in this study. The results indicated that the maximal LA concentration increased from 35.89 ± 1.84 to 42.70 ± 2.18 g/L with the increase of enzyme loading from 0 to 300 U/g VSsubstrate. Mechanism exploration indicated that enzymatic pretreatment significantly promoted the release and hydrolysis of insoluble organic matter from fermentation substrate, thus providing an abundance of reaction intermediates that were directly available for LA production. Additionally, bacteria analysis revealed that the high concentration of LA was associated with the prevalence of Lactobacillus. This study offered an environmental-friendly strategy for promoting SM and AW hydrolysis and provided a viable approach for recovering valuable products.

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