数字农科院2.0

An exonuclease I-assisted quencher-free 2-aminopurine aptasensor based on a multipath paper-based device for ultrasensitive detection of kanamycin

文献类型： 外文期刊

作者： Xuan He;Ji Qi;Dean Song;Xiuli Fu

作者机构：

关键词： 2-Aminopurine;Exonuclease I;Fluorescence;Kanamycin;Paper-based device

期刊名称： Microchemical Journal

ISSN： 0026-265X

年卷期： 2024 年 203 卷

页码：

收录情况： SCIE（2024版）

摘要： A quencher-free multipath microfluidic paper-based analytical device (µPAD) was constructed for ultrasensitive detection of kanamycin based on exonuclease I (Exo I)-assisted signal enhancement of 2-aminopurine (2-AP). Here, Exo I, a single-stranded DNA-specific nuclease, was introduced to fully liberate 2-AP mononucleotides to greatly enhance biosensing sensitivity. 2-AP, a fluorescent adenine analogue embedded in single-stranded DNA (ssDNA), was employed as the detection signal source. The fluorescence of 2-AP is strong in the mononucleotide state, while it can have low fluorescence and even no fluorescence in ssDNA and dsDNA, respectively. The 2-AP fluorescence probe included 2-AP DNA and kanamycin aptamer. When kanamycin was present, binding occurred between kanamycin and the aptamer, leading to ssDNA, which was further digested by Exo I. In this case, free 2-AP mononucleotides were liberated, indicating strong fluorescence. In addition, the captured kanamycin was released for binding with the new aptamer, which resulted in the formation of a binding-hydrolysis-release cycle with the aid of Exo I. Under optimal conditions, this µPAD exhibited sensitive and multipath detection of kanamycin at concentrations as low as 1.26 × 10−14 M with a wide range of 10−13–10−7 M. Furthermore, satisfactory results were achieved for analysing spiked kanamycin in milk and honey samples. This strategy is a very promising tool for monitoring antibiotics and evaluating the safety of animal-derived foods.

分类号：

  • 相关文献

[1]Aptamer Based Fluorometric Determination Of K.anamycin Using Double-Stranded Dna A nd Carbon Nanotubes. Wei, Ben Hua,Luo, Lin Guang,Liao, Qie Gen. 2017

[2]In vitro assay for 2,4-D resistance in transgenic cotton. BAO-HONG ZHANG,HONG-MEI WANG,FANG LIU,YUN-HAI LI,ZHENG-DE LIU.

[3]A Visual Gel-Enzyme-Linked Immunosorbent Assay for Simultaneous Detection of Gentamicin and Kanamycin in Milk. Xu Fei,Liu Mao-Lin,Liu Yi-Ming,Li Xiu-Bo,Li Jing-Ya,Zhou Jie,Ding Shuang-Yang,Wang Jian-Fen. 2015

[4]Effects of kanamycin on tissue culture and somatic embryogenesis in cotton. Bao-Hong Zhang,Fang Liu,Zhi-Hong Liu,Hong-Mei Wang,Chang-Bing Yao.

[5]Study on the interaction between cyanobacteria FBP/SBPase and metal ions. Sun, Yao,Liao, Xun,Li, Ding,Feng, Lingling,Wang, Xiaofeng,Jin, Jing,Yi, Fan,Zhou, Li,Wan, Jian,Li, Jun.

[6]Application of Au based nanomaterials in analytical science. Wang, Peilong,Su, Xiaoou,Tang, Zhiyong,Lin, Zhenyu.

[7]Ultrasensitive detection of lead(II) using a turn-on probe based on the use of an aptamer and a water-soluble fluorescent perylene probe. Yan, Mengmeng,Zhu, Chao,Huang, Yafei,Yan, Jiao,Chen, Ailiang,Yan, Mengmeng,Zhu, Chao,Huang, Yafei,Yan, Jiao,Chen, Ailiang,Huang, Yafei,Yan, Jiao.

[8]A simple and sensitive label-free fluorescent approach for protein detection based on a Perylene probe and aptamer. Lv, Zhenzhen,Liu, Jinchuan,Bai, Wenhui,Yang, Shuming,Chen, Ailiang,Lv, Zhenzhen,Liu, Jinchuan,Bai, Wenhui,Yang, Shuming,Chen, Ailiang,Lv, Zhenzhen.

[9]An Esipt-Based Fluorescent Probe For T.he Determination Of Hypochlorous A cid (Hclo): Mechanism Study And Its Application In Cell Imaging. He, YH, Xu, Y, Shang, YT, Zheng, SW, Chen, WH, Pang, Y. 2018

[10]A Water-Soluble, Small Molecular Fluorescence P.robe Based On 2-(2 ' -Hydroxyphenyl) Benzoxazole For Zn2+ In Plants. Shang, YT, Zheng, SW, Tsakama, M, Wang, M, Chen, WH. 2018

[11]Dual-mode detection of organophosphate pesticides in pear and Chinese cabbage based on fluorescence and AuNPs colorimetric assays. He Yu,Miao Wang,Jing Cao,Yongxin She,Yongan Zhu,Jiaming Ye,A. M. Abd El-Aty,Ahmet Hacımüftüoğlu,Jing Wang,Shuibing Lao. 2021

[12]2-Aminopurine Fluorescence Spectroscopy for Probing a Glucose Binding Aptamer. Lu, Chang,Huang, Po-Jung Jimmy,Zheng, Jingkai,Liu, Juewen. 2022

[13]Development of a time-resolved fluorescence microsphere Eu lateral flow test strip based on a molecularly imprinted electrospun nanofiber membrane for determination of fenvalerate in vegetables. Zhang, Le,Zheng, Yiliu,Shao, Hua,Xiao, Ming,Sun, Jianchun,Jin, Maojun,Jin, Fen,Wang, Jing,Abd El-Aty, A. M.,She, Yongxin. 2022

[14]A naphthimide fluorescent probe for the detection of selenols in selenium-enriched Tan sheep. Liu Y., Feng X., Meng Q., Zhu J., Jia X., Zhao Q., Tang C., Yu Y., Zhang J.. 2022

[15]A Turn-On Fluorescence Sensor Based on Nitrogen-Doped Carbon Dots and Cu2+ for Sensitively and Selectively Sensing Glyphosate. Ziqiang Li,Shuang Liang,Li Zhou,Fengjian Luo,Zhengyun Lou,Zongmao Chen,Xinzhong Zhang,Mei Yang. 2023

[16]Development of a Recombinase Polymerase Amplification Fluorescence Assay for the Detection of Canine Adenovirus 2. Li Xiao,Mengdi Zhang,Zhige Tian,Ye Ge,Tongyuan Zhang,Li Yi,Feng Cong. 2021

[17]Oxidase-like nanozymes-driven colorimetric, fluorescence and electrochemiluminescence assays for pesticide residues. Sha Liu,Chao Nie,Fengjiao He,Guojian Wu,Heng Wang,Si Li,Chenxing Du,Zhi Zheng,Jieshun Cheng,Yizhong Shen,Jie Cheng. 2024

[18]Europium nanospheres based ultrasensitive fluorescence immunosensor for aflatoxin B1 determination in feed. Li H.,Shang Q.,Zhang L.,Mao J.,Zhang Q.,Li P.. 2024

[19]A hydrogel-based ratiometric fluorescent sensor relying on rhodamine B labelled AIE-featured hyperbranched poly(amido amine) for heparin detection. Cheng Li,Bai Tong Liu,Yi Ting Wang,Tian Jiao Zhang,Xiaochun Zheng,Li Chen,Shaobo Li,Xiaoxian Tian,Dequan Zhang,Yong Wang. 2024

[20]Fluorescence/visual aptasensor based on Au/MOF nanocomposite for accurate and convenient aflatoxin B1 detection. Song Liao,Liuyang Gui,Yufan Yang,Yiwei Liu,Xiaopeng Hu. 2024

作者其他论文 更多>>