数字农科院2.0

An extracytoplasmic function sigma/anti-sigma factor system regulates hypochlorous acid resistance and impacts expression of the type IV secretion system in brucella melitensis

文献类型： 外文期刊

作者： Huoming Li;Sen Hu;Xin Yan;Yan Yang;Wenxing Liu;Zhigao Bu;Ganwu Li;Wentong Cai

作者机构：

关键词： Brucella;Brucella melitensis;ECF;Extracytoplasmic function sigma factor;Gene regulation;Type IV secretion;Virulence;Virulence regulation

期刊名称： Journal of Bacteriology

ISSN： 0021-9193

年卷期： 2021 年 203.0 卷 12.0 期

页码：

收录情况： JCR（2021版）

摘要： The intracellular bacterial pathogen Brucella causes persistent infections in various mammalian species. To survive and replicate within macrophages, these bacteria must be able to withstand oxidative stresses and express the type IV secretion system (T4SS) to evade host immune responses. The extracytoplasmic function (ECF) sigma factor system is a major signal transduction mechanism in bacteria that senses environmental cues and responds by regulating gene expression. In this study, we defined an ECFs bcrS and its cognate anti-s factor abcS in Brucella melitensis M28 by conserved domain analysis and a protein interaction assay. BcrS directly activates an adjacent operon, bcrXQP, that encodes a methionine-rich peptide and a putative methionine sulfoxide reductase system, whereas AbcS is a negative regulator of bcrS and bcrXQP. The bcrS-abcS and bcrXQP operons can be induced by hypochlorous acid and contribute to hypochlorous acid resistance in vitro. Next, RNA sequencing analysis and genome-wide recognition sequence search identified the regulons of BcrS and AbcS. Interestingly, we found that BcrS positively influences T4SS expression in an AbcS-dependent manner and that AbcS also affects T4SS expression independently of BcrS. Last, we demonstrate that abcS is required for the maintenance of persistent infection, while bcrS is dispensable in a mouse infection model. Collectively, we conclude that BcrS and AbcS influence expression of multiple genes responsible for Brucella virulence traits. © 2021 Li et al.

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