数字农科院2.0

Exploration of the antimicrobial activities of biocontrol agent Streptomyces strain h114 against Penicillium digitatum in citrus

文献类型： 外文期刊

作者： Tian Z.;Du Y.;Lu Y.;Zhu J.;Long C.-A.

作者机构：

关键词： （0-1-3）Antimicrobial activity;Biocontrol;Citrus;Penicillium digitatum;Streptomyces monashensis h114

期刊名称： Postharvest Biology and Technology

ISSN： 0925-5214

年卷期： 2024 年 210 卷

页码：

收录情况： SCIE（2024版）

摘要： Biocontrol is considerably crucial in phytopathogens management during postharvest fruit storage. In this study, assays were performed to screen beneficial microbes through the microorganisms which were isolated from the citrus rhizosphere soil, and to investigate the potential antifungal action modes towards Penicillium digitatum, the perpetrator of citrus green mold. Strain h114 was selected for its high controlling effect against citrus green mold pathogen, and it was phylogenetically identified as a Streptomyces monashensis isolate by whole genome analysis. Thereafter, a total of 35 secondary metabolite gene clusters which were responsible for the biosynthesis of antibiotics were found in the genome. The culture filtrates (CFS) fermented from strain h114 with incubation for 36 h in beef extract-peptone broth exhibited the strongest antifungal activity towards P. digitatum than filtrates obtained from other time points. Despite the susceptibilities to long time treated by boiled water and high concentration of trypsin, the 36 h fermented CFS showed relatively stable antagonism in room conditions. Further, its xylene-extracted crude compounds also displayed a bioactive effect to P. digitatum. CFS-treated P. digitatum cells exhibited degradation of mitochondria, accompanied by reduced expression of developmental regulatory genes PdBrlA and PdWetA. Moreover, CFS induced the fruit defense activities such as superoxide dismutase and peroxidase, and alleviated the virulence of P. digitatum in citrus. After CFS application, the expansion diameters of P. digitatum on artificially-wounded citrus fruit were reduced by 71.5% at most, and the disease incidence diminished by 62.7–72.9% compared to that of P. digitatum treated fruit alone. Taken together, strain h114 might be a promising bio-fungicide in postharvest citrus fruit handling. © 2023 Elsevier B.V.

分类号：

  • 相关文献

[1]Calcium disodium edetate controls citrus green mold by chelating Mn2+ and targeting pyruvate synthesis in the pathogen. Fan Yang,Shuqi Liu,Zhonghuan Tian,Yujie Du,Deyao Zhang,Chao an Long. 2023

[2]Postharvest biocontrol ability and antagonistic mechanism of Bacillus altitudins h217 against Penicillium digitatum in citrus. Yongqing Lu,Shuqi Liu,Yuqing Wang,Fan Yang,Zhonghuan Tian,Chao an Long. 2024

[3]Genome identification and biocontrol potential against citrus blue mold of Bacillus velezensis w176. Zhonghuan Tian,Yujie Du,Yongqing Lu,Sirong Ji,Chao an Long. 2023

[4]中国葡萄类病毒基因多样性分析及其新种的发现. 姜冬梅,郭瑞,彭山,吴祖建. 2009

[5]中国栽培葡萄上发生的类病毒的种类鉴定. 王晶,SanoTeruo. 2003

[6]PdStuA Is a Key Transcription Factor Controlling Sporulation, Hydrophobicity, and Stress Tolerance in Penicillium digitatum. Yujie Du,Jinfan Zhu,Zhonghuan Tian,Chaoan Long. 2023

[7]PdMesA regulates polar growth, cell wall integrity, and full virulence in Penicillium digitatum of citrus. Shuqi Liu,Xinying Liu,Weiqiang Lai,Yongqing Lu,Yuqing Wang,Chao an Long. 2024

[8]Methylparaben suppresses the citrus postharvest pathogen Penicillium digitatum by undermining the cell wall and membrane and inducing the antioxidant system. Fan Yang,Xiuying Zhong,Yuqing Wang,Juan Zhao,Yongqing Lu,Chao an Long. 2024

[9]Functional analysis of potential fumiquinazoline biosynthetic gene clusters in Penicillium digitatum of citrus. Shuqi Liu,Yongqing Lu,Yuqing Wang,Wei Fang,Chao an Long. 2025

[10]Perillaldehyde controls citrus green mold by inhibiting the ribosome biogenesis of Penicillium digitatum and improving citrus disease resistance. Shuqi Liu,Yuxin Jiang,Jiancheng Sun,Fan Yang,Yuqing Wang,Yongqing Lu,Weiqiang Lai,Chao an Long. 2025

[11]Comparative Analysis Of Tolerant And Susceptible Citrus Reveals The Role Of Methyl Salicylate Signaling In The Response To Huanglongbing. Chen, Shanchun,Xu, Lanzhen,Peng, Aihong,He, Yongrui,Wu, Liu,Peng, Aihong,Wen, Qingli,Xie, Zhu,He, Yongrui,Zou, Xiuping,Chen, Shanchun,Xu, Lanzhen,Xie, Zhu,Wu, Liu,Bai, Xiaojing,Wen, Qingli,Xie, Zhu,Zou, Xiuping,Peng, Aihong,Wu, Liu,Bai, Xiaojing,Zou, Xiuping,Wen, Qingli,Chen, Shanchun,Bai, Xiaojing,He, Yongrui,Xu, Lanzhen. 2019

[12]Modulation Of Tolerance Of Hamlin S.weet Orange Grown On T hree Rootstocks To On-Tree Oleocellosis By Summer Plant Water Balance Supply. Deng, Lie,Yi, Shilai,He, Shaolan,Liu, Yanmei,Zheng, Yongqiang,Wang, Ya,Yang, Qiong,Xie, Rangjin,Ma, Yanyan,Zheng, Yongqiang,Lv, Qiang. 2018

[13]Systematic Analysis Of The Basic/Helix-Loop-Helix (Bhlh) Transcription Factor Family In Pummelo (Citrus Grandis) And Identification Of The Key Members Involved In The Response To Iron Deficiency. Hui, Qiu-Ling,Qiu, Jie-Ya,Zhang, Xiao-Yong,Xu, Yuan-Yuan,Fu, Xing-Zheng,Peng, Liang-Zhi,He, Yi-Zhong,Hui, Qiu-Ling,Qiu, Jie-Ya,Zhang, Xiao-Yong,Xu, Yuan-Yuan,Fu, Xing-Zheng,Peng, Liang-Zhi,He, Yi-Zhong. 2020

[14]An Ecological Research On Potential F.or Zero-Growth Of Chemical F ertilizer Use In Citrus Production In China. Zhang, Zhen-Zhen,Li, Wen-Li,Li, Yu-Jia,Zhou, Peng,Zhang, Yue-Qiang,Zhang, Yue-Qiang,Zhang, Yue-Qiang,Guo, Chao-Yi,Shi, Xiao-Jun,Shi, Xiao-Jun,Shi, Xiao-Jun,Yang, Min,Chen, Xin-Ping,Chen, Xin-Ping,Tang, Xiao-Dong. 2019

[15]Genome-Wide Identification Of Sweet Orange (.Citrus Sinensis) Metal Tolerance P roteins And Analysis Of Their Expression Patterns Under Zinc, Manganese, Copper, And Cadmium Toxicity. Fu, Xing-Zheng,Ling, Li-Li,Fu, Xing-Zheng,Ling, Li-Li,Chun, Chang-Pin,Chun, Chang-Pin,Peng, Liang-Zhi,Fu, Xing-Zheng,Cao, Li,Tong, Ya-Hua,Tong, Ya-Hua,Zhou, Xue,Zhou, Xue,Zeng, Ming,Cao, Li,Peng, Liang-Zhi,Fu, Xing-Zheng. 2017

[16]Abscisic Acid Promotes Jasmonic Acid Accumulation And Plays A Key Role In Citrus Canker Development. He, Yongrui,Li, Qiang,Long, Qin,Zou, Xiuping,Xie, Yu,Chen, Shanchun. 2019

[17]Small Rna Deep Sequencing Reveals F.ull-Length Genome Of Citrus Y ellow Vein Clearing Virus In Chongqing, China. Yu Yun-qi,Wu Qiong,Zhou Chang-yong,Yu Yun-qi,Su Hua-nan,Cao Meng-ji,Wu Qiong,Wang Xue-feng. 2017

[18]Rapid Detection Of Chlorophyll Content A.nd Distribution In Citrus O rchards Based On Low-Altitude Remote Sensing And Bio-Sensors. Deng, Lie,He, Shaolan,Li, Wentao,Lyu, Qiang,Zheng, Yongqiang,Yi, Shilai,Xie, Rangjin,Wang, Kejian,Ma, Yanyan. 2018

[19]Whole-Transcriptome Rna Sequencing Reveals The Global Molecular Responses And Cerna Regulatory Network Of Mrnas, Lncrnas, Mirnas And Circrnas In Response To Copper Toxicity In Ziyang Xiangcheng (Citrus Junos Sieb. Ex Tanaka). Zhang, Xiao-Yong,Fu, Xing-Zheng,Fu, Xing-Zheng,Yuan, Meng,He, Yi-Zhong,Qiu, Jie-Ya,Zhou, Xue,Ling, Li-Li,Peng, Liang-Zhi,Chun, Chang-Pin,Cao, Li,Qiu, Jie-Ya,Chun, Chang-Pin,Zhou, Xue,He, Yi-Zhong,Yuan, Meng,Peng, Liang-Zhi,Ling, Li-Li,Cao, Li,Zhang, Xiao-Yong,Fu, Xing-Zheng,Fu, Xing-Zheng. 2019

[20]Transgenic Citrus Expressing Synthesized Cecropin B. Genes In The P hloem Exhibits Decreased Susceptibility To Huanglongbing. Xu, Lanzhen,Yao, Lixiao,Peng, Aihong,Xu, Lanzhen,Yao, Lixiao,Zou, Xiuping,Zou, Xiuping,Zou, Xiuping,Zou, Xiuping,He, Yongrui,He, Yongrui,Chen, Shanchun,Chen, Shanchun,Jiang, Xueyou,Lei, Tiangang,Peng, Aihong,Jiang, Xueyou,Lei, Tiangang. 2017

作者其他论文 更多>>