数字农科院2.0

Characterization of an Ecdysone Oxidase from Plutella xylostella (L.) and Its Role in Bt Cry1Ac Resistance

文献类型: 外文期刊

作者: Dong, Lina;Zhu, Liuhong;Cheng, Zhouqiang;Bai, Yang;Li, Peixuan;Yang, Hanchi;Tang, Shouwen;Crickmore, Neil;Zhou, Xuguo;Guo, Zhaojiang;Zhang, Youjun

作者机构:

关键词: Plutella xylostella;Bacillus thuringiensis;ecdysone oxidase;20-hydroxyecdysone;Cry1Ac resistance

期刊名称: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

ISSN: 0021-8561

年卷期: 2025 年 73 卷 2 期

页码:

收录情况: SCIE(2025版) ; ; EI(2025版)

摘要: Understanding the molecular mechanisms underlying insect resistance to Bacillus thuringiensis (Bt) pesticidal proteins is crucial for sustainable pest management. Here, we found that downregulation of the Plutella xylostella ecdysone oxidase gene (PxEO) in the normal feeding stages contributes to increased 20-hydroxyecdysone (20E) titer and mediates resistance to the Bt Cry1Ac toxin. The PxEO gene was cloned and its expression was significantly downregulated in the midgut of Bt-resistant and Cry1Ac-selected P. xylostella. Silencing of the PxEO gene significantly reduced Cry1Ac susceptibility, and downregulation of the PxEO gene is closely linked to Cry1Ac resistance in P. xylostella. The PxEO protein metabolized ecdysone (E) and 20E in vitro, and its reduction elevated 20E titers and activated the MAPK-mediated trans-regulatory mechanism known to directly cause the resistance phenotype. Together with our recently reported 20E-degrading glucose dehydrogenase, this finding highlights a robust, multipronged, approach developed by this insect in its 20E-mediated defense against harmful agents.

分类号:

  • 相关文献

[1]The midgut cadherin-like gene is not associated with resistance to Bacillus thuringiensis toxin Cry1Ac in Plutella xylostella (L.). Guo, Zhaojiang,Kang, Shi,Zhu, Xun,Wu, Qingjun,Wang, Shaoli,Xie, Wen,Zhang, Youjun.

[2]Proteomics-based identification of midgut proteins correlated with Cry1Ac resistance in Plutella xylostella (L.). Xia, Jixing,Guo, Zhaojiang,Yang, Zezhong,Zhu, Xun,Kang, Shi,Yang, Xin,Wu, Qingjun,Wang, Shaoli,Xie, Wen,Zhang, Youjun,Xia, Jixing,Xu, Weijun,Yang, Fengshan.

[3]Down-regulation of a novel ABC transporter gene (Pxwhite) is associated with Cry1Ac resistance in the diamondback moth, Plutella xylostella (L.). Guo, Zhaojiang,Kang, Shi,Zhu, Xun,Xia, Jixing,Wu, Qingjun,Wang, Shaoli,Xie, Wen,Zhang, Youjun.

[4]A cis-acting mutation in the pxabcg1 promoter is associated with cry1ac resistance in plutella xylostella (l.). Jianying Qin,Fan Ye,Linzheng Xu,Xuguo Zhou,Neil Crickmore,Xiaomao Zhou,Youjun Zhang,Zhaojiang Guo. 2021

[5]A cis-acting mutation in the pxabcg1 promoter is associated with cry1ac resistance in plutella xylostella (l.). Jianying Qin,Fan Ye,Linzheng Xu,Xuguo Zhou,Neil Crickmore,Xiaomao Zhou,Youjun Zhang,Zhaojiang Guo. 2021

[6]MAPK-Activated Transcription Factor PxJun Suppresses PxABCB1 Expression and Confers Resistance to Bacillus thuringiensis Cry1Ac Toxin in Plutella xylostella (L.). Jianying Qin,Le Guo,Fan Ye,Shi Kang,Dan Sun,Liuhong Zhu,Yang Bai,Zhouqiang Cheng,Linzheng Xu,Chunzheng Ouyang,Lifeng Xiao,Shaoli Wang,Qingjun Wu,Xuguo Zhou,Neil Crickmore,Xiaomao Zhou,Zhaojiang Guo,Youjun Zhang. 2021

[7]MAP4K4 controlled transcription factor POUM1 regulates PxABCG1 expression influencing Cry1Ac resistance in Plutella xylostella (L.). Linzheng Xu,Jianying Qin,Wei Fu,Shaoli Wang,Qinjun Wu,Xuguo Zhou,Neil Crickmore,Zhaojiang Guo,Youjun Zhang. 2022

[8]The role of GPI-anchored membrane-bound alkaline phosphatase in the mode of action of Bt Cry1A toxins in the diamondback moth. Dan Sun,Qiuchen Xu,Le Guo,Yang Bai,Xuping Shentu,Xiaoping Yu,Neil Crickmore,Xuguo Zhou,Alejandra Bravo,Mario Soberón,Youjun Zhang,Zhaojiang Guo. 2025

[9]Characterization of cry9Da4, cry9Eb2, and cry9Ee1 genes from Bacillus thuringiensis strain T03B001. Su, Huiqin,Shu, Changlong,Su, Huiqin,Zhang, Jie,He, Kanglai,Huang, Dafang,Song, Fuping.

[10]Eukaryotic Translation Initiation Factor 2 Modulates the Expression of Midgut Receptors to Confer Resistance to Bacillus thuringiensis Cry1Ac Toxin in Helicoverpa armigera. Zhang, Caihong,Wei, Jizhen,Li, Wenxuan,Li, Ningning,Soe, Ei Thinzar,Naing, Zaw Lin,Tang, Jinrong,Yu, Huan,Fang, Fengyun,Li, Xianchun,Lu, Yanhui,Liu, Xiaoxia,Crickmore, Neil,Liang, Gemei. 2025

[11]The role of 20-hydroxyecdysone and juvenile hormone in insecticidal activity of Bacillus thuringiensis regulated by DUOX-ROS immunity in Spodoptera exigua. Ji, Yujie,Gao, Bo,Zhao, Dan,Zhang, Lu,Wu, Han,Xie, Yifan,Shi, Qiuyu,Wang, Yao,Guo, Wei. 2025

[12]Influences of Cry1Ac Broccoli on Larval Survival and Oviposition of Diamondback Moth. Yi, Dengxia,Cui, Shusong,Yang, Limei,Fang, Zhiyuan,Liu, Yumei,Zhuang, Mu,Zhang, Yangyong,Yi, Dengxia. 2015

[13]High resistance of transgenic cabbage plants with a synthetic cry1Ia8 gene from Bacillus thuringiensis against two lepidopteran species under field conditions. Yi, Dengxia,Yang, Weijie,Tang, Jun,Wang, Li,Fang, Zhiyuan,Liu, Yumei,Zhuang, Mu,Zhang, Yangyong,Yang, Limei,Yi, Dengxia.

[14]Determining the involvement of two aminopeptidase Ns in the resistance of Plutella xylostella to the Bt toxin Cry1Ac: Cloning and study of in vitro function. Chang, Xiaoli,Wu, Qingjun,Wang, Shaoli,Wang, Ran,Yang, Zhongxia,Chen, Defeng,Jiao, Xiaoguo,Mao, Zhenchuan,Zhang, Youjun. 2012

[15]The Minimal Active Fragment of the Cry1Ai Toxin is Located Between 36(I) and 605(I). Lin Hui-yan,Li Ying,Shu Chang-long,Song Fu-ping,Zhang Jie. 2014

[16]Screening of cry-type promoters with strong activity and application in Cry protein encapsulation in a sigK mutant. Zheng, Qingyun,Peng, Qi,Du, Lixin,Shu, Changlong,Zhang, Jie,Song, Fuping,Zhou, Changmei.

[17]Identification of the minimal active fragment of the Cry1Ah toxin. Zhou, Zishan,Song, Fuping,Shu, Changlong,Zhang, Jie,Huang, Dafang.

[18]A versatile contribution of both aminopeptidases N and ABC transporters to Bt Cry1Ac toxicity in the diamondback moth. Dan Sun,Liuhong Zhu,Le Guo,Shaoli Wang,Qingjun Wu,Neil Crickmore,Xuguo Zhou,Alejandra Bravo,Mario Soberón,Zhaojiang Guo,Youjun Zhang. 2022

[19]Plant Polysaccharides Modulate Biofilm Formation and Insecticidal Activities of Bacillus thuringiensis Strains. Mengmeng Li,Changlong Shu,Wang Ke,Xiaoxiao Li,Yiyan Yu,Xiong Guan,Tianpei Huang. 2021

[20]Retrotransposon-mediated evolutionary rewiring of a pathogen response orchestrates a resistance phenotype in an insect host. Zhaojiang Guo,Le Guo,Yang Bai,Shi Kang,Dan Sun,Jianying Qin,Fan Ye,Shaoli Wang,Qingjun Wu,Wen Xie,Xin Yang,Neil Crickmore,Xuguo Zhou,Youjun Zhang. 2023

作者其他论文 更多>>