数字农科院2.0

Bacterial Acetyltransferase Effector AopP2 Primes Effector-Triggered Immunity in Watermelon by Acetylating a Conserved Transcription Factor

文献类型： 外文期刊

作者： Huang, Jingjing;He, Peimin;Zhong, Chen;Qin, Tong;Wang, Hao;Shi, Jiahuan;Wei, Senyi;Chen, Dong;Zhao, Jianlong;Chai, Ali;Kan, Yumin;Yang, Shanshan;Zhang, Xiaoxiao

作者机构：

关键词： AopP2;ClTFIIB2;programmed cell death;watermelon

期刊名称： PLANT CELL AND ENVIRONMENT

ISSN： 0140-7791

年卷期： 2025 年

页码：

收录情况： SCIE（2025版）

摘要： Bacterial fruit blotch (BFB), caused by Paracidovorax citrulli (Pc), threatens global watermelon production, yet genetic resistance remains scarce. This study investigates the potential of non-adapted interaction triggered by Paracidovorax avenae (Pa), a maize pathogen, to combat BFB in watermelon. We demonstrate that Pa strain ATCC 19860 elicits a hypersensitive response (HR) in watermelon via its type III secretion system (T3SS), inducing effector-triggered immunity (ETI). To rapidly screen for Pa type III effectors (T3Es) related to ETI, the nonpathogenic Pseudomonas fluorescens Effector-to-Host Analyzer (EtHAn) strain was used for transient expression of T3Es in watermelon. Among 13 candidate T3Es, the acetyltransferase AopP2 emerged as a potent inducer of programmed cell death (PCD) in watermelon, dependent on its enzymatic activity. AopP2 suppresses reactive oxygen species (ROS) bursts, salicylic acid (SA) signalling, while stabilizing the transcription factor ClTFIIB2 via acetylation, thereby activating ETI. Silencing ClTFIIB2 compromised both basal resistance to Pc and AopP2-induced PCD, whereas transient ClTFIIB2 expression via the EtHAn system enhanced resistance to Pc and AopP2-induced PCD. Notably, pretreatment with low-dose AopP2 primed watermelon defences, significantly reducing Pc proliferation. This study demonstrates that AopP2 suppresses pattern-triggered immunity (PTI) via CITFIIB2 acetylation while triggering ETI, revealing a conserved immune node exploitable for engineering resistance in watermelon. Our findings highlight the potential of non-adapted pathogen effectors as tools for activating ETI to identify disease resistance genes, and provide the first evidence of ClTFIIB2's critical role in watermelon immunity, offering novel strategies for BFB management.

分类号：

  • 相关文献

[1]西瓜EST资源的SSR信息分析及标记开发. 赵胜杰,刘文革,阎志红,何楠,包文风. 2009

[2]施用钾肥对西瓜番茄红素含量的影响. 何楠,赵胜杰,刘文革,阎志红,万学闪. 2009

[3]NaCl胁迫对不同西瓜种质发芽的影响. 阎志红,刘文革,赵胜杰,何楠,王俊良. 2009

[4]不同倍性西瓜果实Vc含量比较研究. 程志强,刘文革,刘志敏,阎志红,赵胜杰,何楠,张俊杰. 2009

[5]不同染色体倍性西瓜花粉形态观察比较. 刘文革,王鸣,阎志红. 2009

[6]Nitric Oxide Content in Wheat Leaves and Its Relation to Programmed Cell Death of Main Stem and Tillers Under Different Nitrogen Levels. Guo Jun-xiang,Chen Er-ying,Yin Yan-ping,Wang Ping,Li Yong,Chen Xiao-guang,Wu Guang-lei,Wang Zhen-lin,Chen Xiao-guang. 2013

[7]Proteomic analysis of PEG-simulated drought stress-responsive proteins of rice leaves using a pyramiding rice line at the seedling stage. Xiong, Jian-Hua,Li, Yang-Sheng,Xiong, Jian-Hua,Fu, Bin-Ying,Xu, Hua-Xue.

[8]Plant ERD2-like proteins function as endoplasmic reticulum luminal protein receptors and participate in programmed cell death during innate immunity. Li, Sizhun,Xie, Ke,Zhang, Qiang,Wang, Yan,Tang, Yang,Liu, Dong,Liu, Yule,Hong, Yiguo,He, Chenyang. 2012

[9]Sulfated lentinan induced mitochondrial dysfunction leads to programmed cell death of tobacco BY-2 cells. Wang, Jie,Shen, Lili,Qian, Yumei,Yang, Jinguang,Wang, Fenglong,Wang, Yaofeng.

[10]Programmed cell death in relation to petal senescence in ornamental plants. Zhou, Y,Wang, CY,Ge, H,Hoeberichts, FA,Visser, PB. 2005

[11]Tungstate: is it really a specific nitrate reductase inhibitor in plant nitric oxide research?. Xiong, Jie,Fu, Guanfu,Yang, Yongjie,Tao, Longxing,Yang, Yongjie,Zhu, Cheng.

[12]Cloning and characterization of a gene encoding cysteine proteases from senescent leaves of Gossypium hirsutum. Yu, SX,Han, XL,Fan, SL.

[13]Bcl-2 suppresses hydrogen peroxide-induced programmed cell death via OsVPE2 and OsVPE3, but not via OsVPE1 and OsVPE4, in rice. Minjuan Deng , Hongwu Bian *, Yakun Xie , Yongho Kim , Wenzhe Wang , Erpei Lin , Zhanghui Zeng , Fu Guo , Jianwei Pan , Ning Han *, Junhui Wang , Qian Qian , Muyuan Zhu *. 2011

[14]Characteristics and functions of glyceraldehyde 3-phosphate dehydrogenase S-nitrosylation during controlled aging of elm and Arabidopsis seeds. Mei Yan Zeng,Yu Qi He,Xue Gao,Yu Wang,Shi Ming Deng,Tian Tian Ye,Xiao Feng Wang,Hua Xue. 2021

[15]Disruption of OsPHD1, Encoding a UDP-Glucose Epimerase, Causes JA Accumulation and Enhanced Bacterial Blight Resistance in Rice. Yu Gao,Xiaojiao Xiang,Yingxin Zhang,Yongrun Cao,Beifang Wang,Yue Zhang,Chen Wang,Min Jiang,Wenjing Duan,Daibo Chen,Xiaodeng Zhan,Shihua Cheng,Qunen Liu,Liyong Cao. 2022

[16]Cereal Endosperms: Development and Storage Product Accumulation. Jinxin Liu,Ming Wei Wu,Chun Ming Liu. 2022

[17]PROGRAMMED CELL DEATH8 interacts with tetrapyrrole biosynthesis enzymes and ClpC1 to maintain homeostasis of tetrapyrrole metabolites in Arabidopsis. Geng, Rudan,Pang, Xiaoqing,Li, Xia,Shi, Shanshan,Hedtke, Boris,Grimm, Bernhard,Bock, Ralph,Huang, Jirong,Zhou, Wenbin. 2023

[18]BAG2 and MAPK2 regulate differently on different periods of heat-induced programmed cell death in tomato. He M.,Jahan M.S.,Khalil H.B.,Wang Y.,Sun J.,Shu S.,Guo S.. 2024

[19]Towards modelling tick-virus interactions using the weakly pathogenic Sindbis virus: Evidence that ticks are competent vectors. Yanan Wang,Zhengmao Xu,Houshuang Zhang,Yongzhi Zhou,Jie Cao,Yuqiang Zhang,Zedong Wang,Jinlin Zhou. 2024

[20]E3 ubiquitin ligase-mediated degradation of Rab GTPase suppresses an MAPKK and activates immunity in rice. Jiang, Su,Gong, Ziwen,Li, Chenggang,Tao, Hui,He, Feng,Xu, Xiao,Wang, Min,Wang, Jisong,Sun, Yuancheng,Feng, Qin,Hao, Zeyun,You, Xiaoman,Wang, Ruyi,Wu, Jun,Wang, Guo-Liang,Xiao, Yinghui,Ning, Yuese,Wang, Dan. 2026

作者其他论文 更多>>