数字农科院2.0

Development and application of a curcumin-cinnamon essential oil nanoemulsion agent against mycobacteria

文献类型： 外文期刊

作者： Lei, Zhihui;Ren, Yixuan;Wang, Jinyao;Shi, Haisu;Lin, Hu;Chen, Hong;Bi, Lijun;Wang, Yutang;Zhang, Hongtai

作者机构：

关键词： curcumin;cinnamon essential oil;nanoemulsion;aerosols;mycobacteria;Mycobacterium tuberculosis

期刊名称： FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY

ISSN： 2235-2988

年卷期： 2025 年 15 卷

页码：

收录情况： SCIE（2025版）

摘要： With the increasing prevalence of drug-resistant Mycobacterium tuberculosis (M. tuberculosis), the development of novel anti-mycobacterial agents has become urgent. In this study, a curcumin-cinnamon essential oil (Cur-CEO) nanoemulsion was developed with optimized preparation parameters, including a 10% CEO volume fraction, 7.5 minutes of ultrasonic treatment, and 350 W ultrasound power, yielding a particle size of 101.14 nm. The nanoemulsion demonstrated high encapsulation efficiency (90.2%) and stability, with a stability coefficient of 0.984. Structural analysis revealed a dense network structure of the nanoemulsion and amorphous forms of Cur and CEO, enhanced by hydrogen bonding and electrostatic interactions, which improved solubility and bioavailability. The Cur-CEO nanoemulsion exhibited potent antimicrobial activity against mycobacteria, demonstrating MIC values of 2 mu g/mL and 0.25 mu g/mL against Mycobacterium smegmatis and M. tuberculosis, respectively, representing a fourfold reduction compared to the CEO solution alone, owing to its ability to induce substantial damage to mycobacterial cell membranes and consequently enhance nucleic acid and protein leakage. Furthermore, the aerosol form of the nanoemulsion effectively inhibited both surface and airborne mycobacteria, with no significant changes in structural properties post-atomization. Lung deposition studies indicated that 75.6% of aerosol particles of the nanoemulsion reached the alveolar region, suggesting its potential as an inhalation agent. Additionally, the Cur-CEO nanoemulsion exhibited negligible effects on macrophage viability, maintaining a survival rate exceeding 85% even at concentrations up to 1250 ng/mL. These findings indicate that the Cur-CEO nanoemulsion, formulated using natural ingredients, holds significant promise as a food-grade antibacterial agent for the prevention and control of mycobacterial infections.

分类号：

  • 相关文献

[1]Incorporation of cinnamon essential oil-loaded Pickering emulsion for improving antimicrobial properties and control release of chitosan/gelatin films. Fan S.,Wang D.,Wen X.,Li X.,Fang F.,Richel A.,Xiao N.,Fauconnier M.-L.,Hou C.,Zhang D.. 2023

[2]Kenaf cellulose nanofiber-based aerogel loading and sustained-release cinnamon essential oil for chilled pork preservation. Xiang, Yuqing,Hu, Yuxiao,Yu, Wang,Huang, Rong,Guo, Xuhong,Tang, Zhonghai,Xing, Chen,Tan, Zhijian. 2025

[3]Nanoemulsion-based delivery systems for nutraceuticals: Influence of carrier oil type on bioavailability of pterostilbene. Sun, Yue,Xia, Ziyuan,Zheng, Jinkai,Qiu, Peiju,McClements, David Julian,Xiao, Hang,Sun, Yue,Qiu, Peiju,Zhang, Lijuan,Zheng, Jinkai,McClements, David Julian.

[4]Improving intracellular uptake of 5-demethyltangeretin by food grade nanoemulsions. Zheng, Jinkai,Li, Yan,Song, Mingyue,McClements, David Julian,Xiao, Hang,Zheng, Jinkai,Li, Yan,Fang, Xiang,Cao, Yong. 2014

[5]Linalool nanoemulsion preparation, characterization and antimicrobial activity against aeromonas hydrophila. Weiming Zhong,Puyu Tang,Ting Liu,Tianyu Zhao,Jiajing Guo,Zhipeng Gao. 2021

[6]Improved storage properties and cellular uptake of casticin-loaded nanoemulsions stabilized by whey protein-lactose conjugate. Bei Zhang,Miao Lei,Wenjing Huang,Gang Liu,Fangcheng Jiang,Dengfeng Peng,Qingrong Huang,Weiping Jin. 2021

[7]Vanillic Acid as a Promising Xanthine Oxidase Inhibitor: Extraction from Amomum villosum Lour and Biocompatibility Improvement via Extract Nanoemulsion. Qian Zhou,Xiaoyan Li,Xiaohui Wang,Dongdong Shi,Shengao Zhang,Yuqi Yin,Hanlin Zhang,Bohao Liu,Nannan Song,Yinghua Zhang. 2022

[8]Study on the bacteriostasis of lemon essential oil and the application of lemon essential oil nanoemulsion on fresh-cut kiwifruit. Chuan Chen,Fan Bing Meng,Han Jia Lv,Zhen Zhen Gou,Jing Qiu,Yun Cheng Li. 2024

[9]Optimization and characterization of pyraclostrobin nanoemulsion for pesticide delivery: Improving activity, reducing toxicity, and protecting ecological environment. Zhang H.,Zhang Y.,Cao C.,Zhao P.,Huang Q.,Cao L.. 2024

[10]Discovery and Characterization of Chitin Synthase Inhibitors with Novel Mechanism for Control of Tetranychus urticae. Wang, Dong,Chen, Wei,Yu, Ailing,Zhang, Zheng,Zhou, Feng,Zhou, Jian,Bian, Qiang,Yang, Qing. 2025

[11]Production of nattokinase and γ-polyglutamic acid via soybean whey fermentation by Bacillus subtilis BSNK-5 and their emulsification to form nanoemulsions. Miao Hu,Pengfei Zhang,Weimin Meng,Jiao Wang,Bei Fan,Fengzhong Wang,Shuying Li. 2024

[12]Astragalus Polysaccharide Nanoemulsion: A Promising Adjuvant for Foot-And-Mouth Disease Virus-Like Particle Vaccines. Xiaoni Shi,Zhidong Teng,Kun Yang,Hetao Song,Yun Zhang,Shuzhen Tan,Hu Dong,Shiqi Sun,Yaozhong Ding,Huichen Guo. 2025

[13]Effect of soy protein isolates obtained by weak-base synchronized membrane separation on nanoemulsion stability: A comparison with alkaline extraction/isoelectric precipitation and salt extraction. Jiaqi Shao,Jing Yang,Ping Wu,Weiping Jin,Junxia Xiao,Lei Wang,Dengfeng Peng,Qianchun Deng. 2025

[14]Characterization of Rv0394c gene encoding hyaluronidase and chondrosulfatase from Mycobacterium tuberculosis. Yuan, Xianglian,Chen, Liping,Cao, Jun,Yu, Shenye,Liu, Siguo,Yuan, Xianglian,Deng, Xiaoxia,Pang, Hai,Wang Quankai. 2013

[15]Development of one-tube multiplex polymerase chain reaction (PCR) for detecting Mycobacterium bovis. Quan, Zhang,Haiming, Tan,Xiaoya, Cai,Weifeng, Yuan,Hong, Jia,Hongfei, Zhu.

[16]Crystallization and preliminary X-ray analysis of a novel esterase Rv0045c from Mycobacterium tuberculosis. Xu, Lipeng,Liu, Siguo,Xu, Lipeng,Guo, Jiubiao,Zheng, Xiangdong,Pang, Hai,Wen, Tingyi,Sun, Fei. 2010

[17]Expression, Purification and Characterisation of Secreted Esterase Rv2525c from Mycobacterium tuberculosis. Dang, Guanghui,Chen, Liping,Li, Zhaoli,Cui, Yingying,Cao, Jun,Yu, Shenye,Liu, Siguo,Deng, Xiaoxia,Pang, Hai.

[18]C-terminal heat shock protein 70 of Mycobacterium tuberculosis as a molecular adjuvant for DNA vaccination with the porcine circovirus type 2 ORF2 (capsid) gene in mice. Fu, Fang,Tian, Huabin,Li, Xuesong,Lang, Yuekun,Liu, Siguo,Li, Haizhong,Wang, Wei,Li, Xi,Chen, Xin,Li, Xuesong,Tong, Guangzhi. 2013

[19]A novel B cell epitope in cold-shock DEAD-box protein A from Mycobacterium tuberculosis. Wang, Huanan,Zhu, Ting,Yu, Shenye,Liu, Huifang,Wang, Xiumei,Chen, Liping,Si, Wei,Liu, Siguo,Wang, Huanan,Pang, Hai.

[20]Characterization of a novel exported esterase Rv3036c from Mycobacterium tuberculosis. Chen, Liping,Dang, Guanghui,Cao, Jun,Yu, Shenye,Liu, Siguo,Dang, Guanghui,Deng, Xiaoxia,Pang, Hai,Dang, Guanghui,Wu, Defeng.

作者其他论文 更多>>