数字农科院2.0

CRISPR-Cas12a在病原快速检测中的应用

文献类型： 中文期刊

作者： 周旭;王思文;王秀荣

作者机构：

关键词： CRISPR-Cas系统;Cas12a;病原核酸检测

期刊名称：中国兽医科学

ISSN： 1673-4696

年卷期： 2022 年

页码：

收录情况： 北大核心（2020版） ; CSCD（2021-2022年度） ; 科技核心（2021版） ; 农林核心（2020版）

摘要： 近年来，CRISPR-Cas系统病原核酸检测技术，凭借其靶向精准、灵敏度高以及多重检测等特点，在即时检测（PointofCaretesting，POCT）领域受到广泛关注。多种Cas蛋白具有顺式基因编辑功能和非特异性反式切割活性，基于这种特性成功实现了病原核酸检测，极大改善了POCT产品灵敏度低的问题。其中，以Cas12为靶点并反式切割单链DNA 探针的系统既简单又高效。因此，就CRISPR-Cas12a的历史、分类、检测原理、在核酸检测中的应用进展、开发检测试剂的困难等方面做一综述。

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