数字农科院2.0

德氏假单胞R-8菌脱硫的"硫饥饿"诱导机理

文献类型： 中文期刊

作者： 余志坚;李焕杰;王海胜;李玉光;闫艳春;李信

作者机构：

关键词： 德氏假单胞菌;硫酸盐;DBT;"硫饥饿"诱导;pPR9TT;脱硫基因

期刊名称：应用与环境生物学报

ISSN： 1006-687X

年卷期： 2009 年 15 卷 2 期

页码： 235-239

收录情况： CSCD

摘要： 以专一性脱硫菌德氏假单胞菌(Pseudomonas delafieldii)R-8为出发菌株,构建了含有重组质粒pRT-D的重组菌株R-8-D.在不同硫酸盐浓度条件下,研究了R-8和R-8-D脱硫的"硫饥饿"诱导机理.结果表明,在充足的Na_2SO_4(＞0.023 mmol L~(-1))条件下,R-8菌首先利用硫酸盐生长,脱硫酶的合成受阻遏,DBT不被利用,而R-8菌处于"硫饥饿"状态(Na_2SO_4浓度≤0.023 mmol L~(-1))时,诱导了脱硫酶的合成,能利用DBT生长;Na_2SO_4在临界浓度以上时,R-8-D菌阻遏了脱硫基因的报告基因lacZ的表达,低于临界浓度时不抑制lacZ表达.本实验结果从细胞和分子水平上证实了R-8菌脱硫属"硫饥饿"诱导类型,并首次确定了脱硫微生物"硫饥饿"诱导的硫酸盐临界浓度为0.023 mmol L~(-1),为构建高活性的、不受硫酸盐抑制的脱硫工程菌提供了理论依据和技术支持.图10表1参14

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