数字农科院2.0

甘蓝型特高含油量油菜种质及其主栽品种的指纹图谱构建

文献类型： 中文期刊

作者： 赵卫国;王灏;李殿荣;田建华;赵亚军;李保军;王晓东;王爱娜

作者机构：

关键词： 甘蓝型油菜;特高含油量;SSR;指纹图谱构建

期刊名称：植物遗传资源学报

ISSN： 1672-1810

年卷期： 2011 年 12 卷 06 期

页码： 904-909

收录情况： 北大核心 ; CSCD

摘要： 选择特高含油量资源7份,与中国各油菜主产区具有代表性的主栽品种16份,利用SSR多引物组合法开展指纹图谱构建研究。选择多态丰富、图谱清晰稳定且来自不同连锁群的引物28对,对所有材料进行指纹图谱分析,共获SSR指纹条带302条,其中多态性条带为279条,每引物所获条带6～16条,平均10.79条,平均多态率92.38%,通过指纹图谱将所有材料有效地区别开来。用非加权类平均法(UPGAM)聚类分析显示,高油材料之间以及高油材料与主栽品种之间遗传距离均有较大差异,在遗传距离0.171处可将23份材料分成9个类群,其中7份高油材料分处4个类群,遗传距离差异显著;而其他8份主栽品种被分别聚类在另外5个类群中,主栽区域特别明显;所有材料间皆具有丰富的遗传多样性,其中高油材料与主栽品种间遗传差异更大。

分类号： S565.4

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