数字农科院2.0

基于茶树SNP的dCAPS标记体系研究

文献类型： 中文期刊

作者： 张成才;王丽鸳;韦康;成浩;包云秀;刘本英;汪云刚

作者机构：

关键词： 茶树;EST;SNP;dCAPS

期刊名称：茶叶科学

ISSN： 1000-369X

年卷期： 2012 年 32 卷 06 期

页码： 51-56

收录情况： 北大核心 ; CSCD

摘要： 从茶树EST序列中搜寻出候选SNPs位点,在候选SNPs两侧设计测序引物进行PCR扩增,并对扩增产物双向测序验证;然后从每个扩增子中选择一个确证的SNPs,设计dCAPS引物,并进行扩增和酶切验证,将SNPs转化为dCAPS标记;最后,在长叶白毫×福鼎大白的F1群体中检测了标记的分离情况。结果发现,20对测序引物中有11对扩增成功,共确证了17个SNPs,占检测总数的54.8%;在设计的11对dCAPS引物中,有8对在长叶白毫、福鼎大白和龙井43等8个品种中表现出多态性,成功转化为dCAPS标记,转化成功率72.7%;8个dCAPS标记中的DCC229和DCC371在长叶白毫和福鼎大白之间表现有多态性,经检测,它们在这2个品种的F1群体中均符合孟德尔1︰1分离。

分类号： S571.1

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