数字农科院2.0

与番茄Ps-2位点紧密连锁的AFLP分子标记的获得

文献类型: 中文期刊

作者: 李君明;朱莎;宋燕;张智;Bistra Atanassova;张盛林;徐和金

作者机构:

关键词: 番茄;Ps-2位点;AFLP

期刊名称:植物遗传资源学报

ISSN: 1672-1810

年卷期: 2006 年 7 卷 01 期

页码: 35-38

摘要: 含有ps-2雄性不育基因的番茄材料可通过自交获得100%的不育后代,方便地应用于番茄杂交制种。作者以来自保加利亚含有ps-2基因的不育材料CMC1ps2为母本,以优良的加工番茄92155为父本,构建了123个F2分离群体,育性表型分离比例完全符合孟德尔遗传规律,为单基因隐性遗传。采用AFLP分子标记技术,通过筛选64对AFLP引物组合,获得了与可育ps-2位点紧密连锁的3个AFLP标记(E37/M47、E38/M48及E33/M50),它们与可育基因的遗传图距分别是6.04cM、6.04cM、6.06cM,而且位于可育基因的同一侧,位置基本相同,分别扩增出390bp、150bp、80bp的DNA片断。这3个标记的获得,可直接用于标记辅助选育,加速番茄雄性不育的转育和利用。

分类号: S641.2

  • 相关文献

[1]番茄分子遗传图谱构建和晚疫病抗性基因簇ph-3的QTL分析. 黄晓梅,许向阳,李景富,陈秀玲,徐艳辉. 2009

[2]增强番茄果实颜色基因的精细定位. 王孝宣,杜永臣,朱德蔚,高建昌,LuigiMonti,S.Grandillo. 2004

[3]鲜食番茄茄红素基因的AFLP-SCAR分子标记. 侯丽霞,刘淑梅,王施慧,吕鑫. 2011

[4]Amplified fragment length polymorphism analysis of Mythimna separata (Lepidoptera : Noctuidae) geographic and melanie laboratory populations in China. Luo, Li-Zhi,Zhang, Lei.

[5]Targeted BSA mapping of Scmv1 and Scmv2 conferring resistance to SCMV using PstI/MseI compared with EcoRI/MseI AFLP markers. Yuan, L,Dussle, CM,Muminovic, J,Melchinger, AE,Lubberstedt, T. 2004

[6]Genetic variation in the invasive process of Bursaphelenchus xylophilus (Aphelenchida : Aphelenchoididae) and its possible spread routes in China. Cheng, X-Y,Cheng, F-X,Xu, R-M,Xie, B-Y. 2008

[7]Autopolyploidy leads to rapid genomic changes in Arabidopsis thaliana. Yang, Yan,Wei, Fang,Duan, Jifa,Braynen, Janeen,Tian, Baoming,Cao, Gangqiang,Shi, Gongyao,Yuan, Jiachen,Yang, Yan,Tian, Baoming.

[8]AFLP fingerprinting of Chinese epidemic strains of Puccinia striiformis f. sp tritici. Liu, F,Kang, ZS,Chen, SY,Li, ZQ,Wu, LR. 2001

[9]Isolation and detection of differential genes in hot pepper (Capsicum annuum L.) after space flight using AFLP markers. Xie, Libo,Wang, Xue,Zhou, Yu,Chen, Lixin,Gao, Yongli,Guo, Yahua,Peng, Mu,Meng, Fanjuan,Liu, Luxiang.

[10]Strain-typing of Lentinula edodes in China with inter simple sequence repeat markers. Zhang, Ruiying,Huang, Chenyang,Zheng, Suyue,Zhang, Jinxia,Ng, Tzi Bun,Jiang, Ruibo,Zuo, Xuemei,Wang, Hexiang.

[11]Molecular Characterization of Genetic Diversity of Underutilized Crops: Buckwheat as an Example. Zhang, Zongwen,Zhao, Lijuan. 2013

[12]Genetic evidence of local adaptation of wheat yellow rust (Puccinia striiformis f. sp tritici) within France. Duan, X,Leconte, M,Hovmoller, MS,De Vallavieille-Pope, C.

[13]Molecular variation among Chinese and global germplasm in spring faba bean areas. Redden, R.,Zong, X.,Ren, J.,Guan, J.,Wang, S.,Zong, X.,Liu, Q.,Paull, J. G..

[14]Evidence for tetrasomic inheritance in a tetraploid Solarium commersonii (+) S-tuberosum somatic hybrid through the use of molecular markers. Barone, A,Li, J,Sebastiano, A,Cardi, T,Frusciante, L. 2002

[15]Effects of abamectin selection on the genetic differentiation within Plutella xylostella (Lepidoptera: Plutellidae) based on amplified fragment length polymorphism. Zhou, Xiao-Mao,Wu, Qing-Jun,Zhang, You-Jun,Zhou, Xiao-Mao,Bai, Lian-Yang,Huang, Xiong-Ying. 2010

[16]DNA Template Preparation and Technology System Building for AFLP Analysis of Chinese Cabbage (Brassica campestris L. ssp pekinensis). Meng, Shuchun,Zhang, Haiying,Liu, Ling,Liu, Yumei. 2012

[17]Genetic diversity in tetraploid switchgrass revealed by AFLP marker polymorphisms. Todd, J.,Wu, Y. Q.,Wang, Z.,Samuels, T.,Wang, Z.. 2011

[18]Sequencing the Potato Genome: Outline and First Results to Come from the Elucidation of the Sequence of the World's Third Most Important Food Crop. Visser, Richard G. F.,Bachem, Christian W. B.,de Boer, Jan M.,Tang, Xiaomin,Bryan, Glenn J.,Chakrabati, Swarup K.,Feingold, Sergio,Gromadka, Robert,van Ham, Roeland C. H. J.,Huang, Sanwen,Jacobs, Jeanne M. E.,Kuznetsov, Boris,de Melo, Paulo E.,Milbourne, Dan,Orjeda, Gisella,Sagredo, Boris. 2009

[19]Development of salinity-tolerant wheat recombinant lines from a wheat disomic addition line carrying a Thinopyrum junceum chromosome. Wang, RRC,Li, XM,Hu, ZM,Zhang, JY,Larson, SR,Zhang, XY,Grieve, CM,Shannon, MC. 2003

[20]Taxonomic and phylogenetic relationships among the genera of the True Citrus Fruit Trees Group (Aurantioideae, Rutaceae) based on AFLP markers. Xie, Rang-Jin,Zhou, Zhi-Qin,Deng, Lie,Xie, Rang-Jin,Zhou, Zhi-Qin. 2008

作者其他论文 更多>>