数字农科院2.0

牛类胰岛素生长因子结合蛋白3基因PCR-SSCP分析

文献类型： 中文期刊

作者： 高雪;徐秀容;许尚忠;任红艳;张英汉

作者机构：

关键词： 牛;类胰岛素生长因子结合蛋白3(IGFBP3)基因;遗传多态;PCR-SSCP

期刊名称：农业生物技术学报

ISSN： 1006-1304

年卷期： 2006 年 14 卷 04 期

页码： 474-477

收录情况： CSCD

摘要： 采用PCR-SSCP技术分析了牛类胰岛素生长因子结合蛋白3(IGFBP3)基因在南阳牛、鲁西牛和中国西门塔尔牛3个牛品种中的遗传多态性。结果表明:IGFBP3的第3外显子不存在遗传多态性;第2外显子在3个牛品种中检测到了AA、AB和BB基因型,而且A等位基因为3个牛群体的优势等位基因,分布较高。3个品种中,中国西门塔尔牛AA基因型频率最高,达到0.6615,而鲁西牛和南阳牛则相对较低,分别为0.4043和0.3095。对第2外显子的多态片段测序分析表明:位于IGFBP3基因第8069bp处发生单碱基突变T→C,并导致了苯丙氨酸变为亮氨酸。

分类号： S823

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