数字农科院2.0

布鲁氏菌LAMP检测方法的建立

文献类型： 中文期刊

作者： 蔺国珍;邱昌庆;郑福英;周继章;曹小安;宫晓炜;王光华

作者机构：

关键词： 布鲁氏菌;LAMP

期刊名称：第3届全国人畜共患病学术研讨会论文集

ISSN：

年卷期： 2011 年

页码： 1

收录情况： CSCD

摘要： 本研究旨在建立一种灵敏、特异、快速高效的布鲁氏菌体外等温扩增检测方法。首先基于新兴的环介导等温扩增技术,参考GenBank中流产布鲁氏菌omp25基因序列,应用DNAStar软件分析其序列,利用PrimerExplorerV3软件,设计了4条特异性引物,即2条内外引物和2条内引物。然后提取阳性质粒DNA作为模板,对引物、反应温度、时间等几个重要参数进行优化。试验结果表明:该方法对于耶尔森菌、大肠杆菌、根瘤菌等无非特异性扩增,表现出良好的特异性;敏感性高,最低可扩增到9fg/μl的DNA,比普通PCR至少高出10倍;比PCR用时更短,可在2h内完成;而且简单易行,不需要复杂的仪器,常规水浴...

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