数字农科院2.0

桃花芽休眠解除相关上调表达基因的cDNA-AFLP分析

文献类型: 中文期刊

作者: 罗静;张巧莲;武峥;漆巨容;谢永红

作者机构:

关键词: 桃;休眠;差异表达基因;cDNA-AFLP

期刊名称:果树学报

ISSN: 1009-9980

年卷期: 2012 年 04 期

页码: 39-44

收录情况: 北大核心 ; CSCD

摘要: 【目的】桃在冬天为了避免低温伤害而停止生长的发育过程称之为自然休眠。为研究桃花芽休眠解除的分子遗传机制,筛选休眠相关基因。【方法】以中需冷量桃品种‘满天红’为试材,采用cDNA-AFLP技术鉴定花芽内差异表达的基因,并对上调基因进行功能预测。【结果】12对引物共扩增出525条差异TDFs(transcripts-derived fragment),其中与休眠解除相关的上调TDFs 14条,下调的TDFs 18条。对休眠解除后上调的14个差异条带进行克隆测序,经Blast显示其中10个基因分别与泛素连接酶E3、植物组蛋白H3、核糖体蛋白L34、Prohibitin-2蛋白以及半乳糖基转移酶等基因同源性较高。【结论】筛选到的休眠相关基因功能涉及代谢、逆境响应、细胞分化及信号转导,这些基因有助于我们更好的解析桃花芽休眠的机制。

分类号: S662.1

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