数字农科院2.0

山葡萄基因组DNA提取及RAPD鉴定

文献类型: 中文期刊

作者: 王军;贺普超

作者机构:

关键词: 山葡萄;基因组DNA提取;RAPD

期刊名称:果树科学

ISSN: 41-1160/S

年卷期: 2000 年 17 卷 02 期

页码: 79-82

收录情况: 北大核心 ; CSCD

摘要: 以雌花山葡萄(73064)冷藏幼叶、香妃葡萄新鲜幼叶为试材,分别采用修改的CTAB法、高盐法、SDS法提取基因组DNA。结果表明,三种方法所提的DNA纯度及产率有差别,从综合结果看,以CTAB法为好,所得DNA不需经氯化艳梯度离心或柱层析,可直接用于RAPD分析。

分类号: Q946.

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