山葡萄基因组DNA提取及RAPD鉴定
文献类型: 中文期刊
作者: 王军;贺普超
作者机构:
关键词: 山葡萄;基因组DNA提取;RAPD
期刊名称:果树科学
ISSN: 41-1160/S
年卷期: 2000 年 17 卷 02 期
页码: 79-82
收录情况: 北大核心 ; CSCD
摘要: 以雌花山葡萄(73064)冷藏幼叶、香妃葡萄新鲜幼叶为试材,分别采用修改的CTAB法、高盐法、SDS法提取基因组DNA。结果表明,三种方法所提的DNA纯度及产率有差别,从综合结果看,以CTAB法为好,所得DNA不需经氯化艳梯度离心或柱层析,可直接用于RAPD分析。
分类号: Q946.
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