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应用PCR技术鉴定我国旋毛虫分离株的研究

文献类型: 中文期刊

作者: 付宝权;刘明远;姚春雨;李文卉;李永光;王艳华;吴秀萍;张德林;才学鹏;RaduBlaga;PascalBoireau

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关键词: 中国旋毛虫分离株;PCR;鉴定

期刊名称:中国畜牧兽医学会家畜寄生虫学分会第六次代表大会暨第十次学术研讨会论文集

ISSN:

年卷期: 2009 年

页码: 1

收录情况: CSCD

摘要: 根据旋毛虫T.spiralis(T1)和乡土旋毛虫T.nativa(T2)的线粒体核糖体小亚基及大亚基RNA基因序列设计合成特异性引物,同时合成旋毛虫核糖体扩展片段V特异引物,以旋毛虫T.spiralis和乡土旋毛虫T.nativa标准株为对照,对我国10个旋毛虫分离株进行了PCR鉴定。结果表明,河南猪株、西安猪株、云南猪株、哈尔滨猪株、天津猪株、乌兰浩特犬株1旋毛虫的PCR扩增条带与T1一致,为旋毛虫T.spiralis;而长春犬株与鸟兰浩特犬株2旋毛虫的PCR扩增条带与T2一致,为乡土旋毛虫T.nativa。同时对以上旋毛虫分离株5SrDNA内部基因间隔区序列进行测定,序列比对分析结果也...

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