数字农科院2.0

ROTIS: A RUBY-assisted One-shot Tissue-culture-free System for In-planta Soybean-transformation from Germinated Seedlings

文献类型: 外文期刊

作者: 李永光;陆珂;冯顺利;宁燃;李海燕;赵雪;和玉兵;韩英鹏

关键词: Transformation; Soybean; RUBY; Tissue-culture-free; In-planta

期刊名称: PLANT PHYSIOLOGY

ISSN: 0032-0889

年卷期: 2025 年

页码:

收录情况: SCIE(2025版)

摘要: Genetic transformation is a fundamental tool for crop improvement, functional genomics, and molecular breeding. Conventional soybean transformation techniques remain constrained by genotype dependence, technical complexity, and stringent sterility requirements. To overcome these constraints, we devised a streamlined system, named RUBY-assisted One-shot Tissue-culture-free In-planta Soybean- transformation (ROTIS). ROTIS exploited germinated seedlings as explants and enables Agrobacterium-mediated transformation under non-sterile conditions. This approach rapidly elicits adventitious shoots while offering three key advantages: (i) markedly simplified workflows, (ii) a condensed 40-80 day flow to generate transgenic plants, and (iii) the capacity to yield multiple independent events per treated seedling. Critically, the protocol exhibits robust efficacy across a broad spectrum of soybean genotypes, effectively circumventing the genotype-specific barriers inherent in traditional approaches. Consequently, this advancement significantly improves transformation efficiency and scalability for soybean genetic improvement and breeding applications.

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