数字农科院2.0

番茄抗番茄花叶病毒和斑点萎凋病毒病基因PCR标记的同时鉴定

文献类型: 中文期刊

作者: 陈丽静;李君明;宋燕;李天来;徐和金;周永健

作者机构:

关键词: 番茄;PCR;Tm22基因;Sw-5基因

期刊名称:中国农业科学

ISSN: 0578-1752

年卷期: 2004 年 37 卷 07 期

页码: 982-986

收录情况: 北大核心 ; CSCD

摘要: 利用同一PCR反应体系,对分别与番茄的抗番茄花叶病毒病的Tm22基因和抗斑点萎凋病毒病的Sw-5基因紧密连锁的SCAR标记进行了同时扩增筛选,扩增的特异性片段与单引物扩增片段完全吻合,其中与Tm22基因紧密连锁的SCAR1标记为共显性标记,抗感试材均产生800bp的特异片段,杂合抗病基因型和感病基因型有HindIII酶切位点,酶切结果为:纯合抗病RR:950bp;杂合抗病Rr:950bp+500bp+300bp+150bp;感病的rr:500bp+300bp+150bp,纯合抗病基因型无HindIII酶切位点。与Sw-5基因紧密连锁的SCAR2标记为显性标记,只有抗病试材扩增出400bp的特异性片段。经反复验证,结果稳定、准确可靠,可用于在同一PCR反应体系中对2个抗病基因进行同时筛选鉴定。该体系的建立不仅省时、省工、节省费用,而且可用于苗期早期辅助选育,加快番茄育种进程。

分类号: S436.41

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