数字农科院2.0

Box-Behnken法优化人参皂苷Rg1、Re、Rb1提取工艺

文献类型： 中文期刊

作者： 赵立春;刘继永

作者机构：

关键词： 人参皂苷;超声提取法;Box-Behnken法;UPLC-MS;提取率

期刊名称：食品工业

ISSN： 1004-471X

年卷期： 2020 年 NULL 卷 1 期

页码： 162-167

摘要： 采用Box-Behnken法优化人参中3种人参皂苷超声提取工艺.以3种人参皂苷Rg1、Re、Rb1 (2015版中国药典)提取率作响应值,通过单因素试验分别考察提取溶剂、料液比、超声功率、超声温度、超声时间、超声次数对结果的影响,通过Box-Behnken法设计四因素三水平试验,采用UPLC-MS分析检测,通过对3种人参皂苷进行正、负离子的全扫检测(MRM),获得响应值,优化提取工艺.结果表明:以70%乙醇作提取剂,超声3次,当超声功率为480 W,温度为60℃,料液比为1︰50 (g/mL),超声时间为30 min,人参皂苷Rg1、Re、Rb1的提取率达到最高,分别为3.10, 3.44和2.45 mg/g.且3种人参皂苷的回收率分别为96%, 103%和98.1%,回收率较好.Box-Behnken法优化3种人参皂苷提取工艺方法简便,回收率较高,优化具有可行性.

分类号： R284.2

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