数字农科院2.0

口蹄疫病毒非结构蛋白3AB基因的可溶性表达与反应原性的鉴定

文献类型: 中文期刊

作者: 祝秀梅;卢曾军;胡永浩;曹轶梅;马江涛;刘在新

作者机构:

关键词: 口蹄疫病毒;非结构蛋白3AB;ELISA

期刊名称:农业生物技术学报

ISSN: 1006-1304

年卷期: 2008 年 16 卷 01 期

页码: 15-19

收录情况: 北大核心 ; CSCD

摘要: 设计特异性引物扩增得到完整的口蹄疫病毒(FMDV)非结构蛋白(NSP)3AB基因,定向克隆到表达载体pET-30a中,获得了重组质粒pET-3AB,并转化大肠杆菌(Escherichia coli)BL21(DE3),重组菌分别在37℃和28℃条件下诱导表达,表达产物经SDS-PAGE电泳鉴定,观察到37℃培养条件下目的蛋白形成包涵体,在28℃条件下培养目的蛋白以可溶性的形式表达。Western blot分析表明,表达的目的蛋白能与FMDV感染牛血清发生特异性反应。以纯化的3AB蛋白作为抗原,采用间接ELISA模式检测了部分FMDV感染动物血清和健康动物血清,证明表达蛋白与FMDV感染血清有很好的反应而与健康动物血清无反应。

分类号: S852.65

  • 相关文献

[1]口蹄疫病毒非结构蛋白3AB单克隆抗体的制备及其对应表位区分析. 刘丹丹,高明春,宋军,宋鸽,曹永生,张润祥,李爽,于力,王君伟. 2011

[2]检测猪血清口蹄疫病毒非结构蛋白3AB抗体ELISA方法的建立. 祝秀梅,卢曾军,胡永浩,曹轶梅,马江涛,刘在新. 2008

[3]口蹄疫病毒非结构蛋白3B单克隆抗体(MAb)的制备及基于MAbELISA方法的初步建立. 李超斯,周国辉,孙静,刘云,于力. 2014

[4]口蹄疫病毒抗体SA-ELISA快速检测方法的建立. 周广青,刘晓庆,史喜绢,杨大鹏,袁莉刚,常惠芸. 2023

[5]A型塞内卡病毒3AB蛋白原核表达及间接ELISA方法的初步建立. 郑君佐,张中旺,马中元,梁志博,白英杰,赵永聪,潘丽,包世俊,吕建亮. 2024

[6]Evaluation of recombinant granule antigens GRA1 and GRA7 for serodiagnosis of Toxoplasma gondii infection in dogs. Wang, Zedong,Huang, Si-Yang,Zhu, Xing-Quan,Wang, Zedong,Ge, Wei,Li, Jiping,Liu, Quan. 2014

[7]Occurrence of several main antibiotic residues in raw milk in 10 provinces of China. Zheng, Nan,Wang, Jiaqi,Han, Rongwei,Xu, Xiaomin,Zhen, Yunpeng,Qu, Xueyin,Sun, Peng,Li, Songli,Han, Rongwei,Yu, Zhongnuo. 2013

[8]Consumption of Bt rice pollen expressing Cry2Aa does not cause adverse effects on adult Chrysoperla sinica Tjeder (Neuroptera: Chrysopidae). Wang, Yuanyuan,Li, Yunhe,Chen, Xiuping,Zhang, Jie,Chen, Hongyin,Peng, Yufa,Romeis, Joerg. 2012

[9]Survey of aflatoxin M1 in raw milk in the five provinces of China. Zheng, Nan,Wang, Jia-Qi,Han, Rong-Wei,Sun, Peng,Zheng, Nan,Wang, Jia-Qi,Han, Rong-Wei,Zhen, Yun-Peng,Xu, Xiao-Min,Zheng, Nan,Wang, Jia-Qi,Han, Rong-Wei,Zhen, Yun-Peng,Xu, Xiao-Min,Han, Rong-Wei. 2013

[10]Preparation and Identification of a Monoclonal Antibody Specific to a Broad Spectrum of O, O-diethyl Organophosphorus Pesticides. Zhang Li-Jie,Pan Jia-Rong. 2010

[11]Molecular identification and antigenic characterization of a merozoite surface antigen and a secreted antigen of Babesia canis (BcMSA1 and BcSA1). Zhou, Mo,Cao, Shinuo,Luo, Yuzi,Liu, Mingming,Wang, Guanbo,Moumouni, Paul Franck Adjou,Jirapattharasate, Charoonluk,Iguchi, Aiko,Vudriko, Patrick,Terkawi, Mohamad Alaa,Nishikawa, Yoshifumi,Suzuki, Hiroshi,Igarashi, Ikuo,Xuan, Xuenan,Cao, Shinuo,Lowenstein, Mario,Kern, Angela. 2016

[12]Development of a Monoclonal Antibody-Based icELISA for the Detection of Ustiloxin B in Rice False Smut Balls and Rice Grains. Fu, Xiaoxiang,Wang, Ali,Wang, Xiaohan,Lin, Fengke,He, Lishan,Lai, Daowan,Zhou, Ligang,Wang, Baoming,Liu, Yang,Li, Qing X.. 2015

[13]Tier-1 assays for assessing the toxicity of insecticidal proteins produced by genetically engineered plants to non-target arthropods. Romeis, Joerg,Wu, Kong-Ming,Peng, Yu-Fa,Romeis, Joerg. 2014

[14]Development of Bt Rice and Bt Maize in China and Their Efficacy in Target Pest Control. Liu, Qingsong,Peng, Yufa,Li, Yunhe,Hallerman, Eric. 2016

[15]A one-step multiplex RT-PCR assay for simultaneous detection of four viruses that infect peach. Zhao, Z.,Jiang, D.,Li, S.,Yu, Y.,Jiang, D.,Wu, Z.,Zhao, Z.. 2013

[16]Preparation of monoclonal antibody and development of an indirect competitive enzyme-linked immunosorbent assay for ornidazole detection. Zhao, Liang,Li, Jiaying,Li, Ye,Wang, Tiangang,Rahman, Ebeydulla,Ji, Baoping,Zhou, Feng,Jin, Xiaolu,Wang, Kai,Xing, Yuan,Xing, Yuan.

[17]Evaluation of recombinant multi-epitope proteins for diagnosis of goat schistosomiasis by enzyme-linked immunosorbent assay. Lv, Chao,Hong, Yang,Fu, Zhiqiang,Lu, Ke,Cao, Xiaodan,Wang, Tao,Zhu, Chuangang,Li, Hao,Xu, Rui,Jia, Bingguang,Han, Qian,Dou, Xuefeng,Shen, Yuanxi,Zhang, Zuhang,Zai, Jinli,Feng, Jintao,Lin, Jiaojiao,Lin, Jiaojiao,Lv, Chao,Dou, Xuefeng,Shen, Yuanxi. 2016

[18]Enzyme-linked immunosorbent assays using recombinant TgSAG2 and NcSAG1 to detect Toxoplasma gondii and Neospora caninum-specific antibodies in domestic animals in Turkey. Zhou, Mo,Cao, Shinuo,Liu, Mingming,Wang, Guanbo,Moumouni, Paul Franck Adjou,Jirapattharasate, Charoonluk,Suzuki, Hiroshi,Nishikawa, Yoshifumi,Xuan, Xuenan,Zhou, Mo,Sevinc, Ferda,Sevinc, Mutlu,Ceylan, Onur.

[19]Development and evaluation of two truncated recombinant NP antigen-based indirect ELISAs for detection of bovine parainfluenza virus type 3 antibodies in cattle. Yang, Yong,Wang, Feng-Xue,Sun, Na,Cao, Li,Zhang, Shu-Qin,Zhu, Hong-Wei,Guo, Li,Cheng, Shi-Peng,Wen, Yong-Jun.

[20]Evaluation of spore wall protein 1 as an alternative antigen for the diagnosis of Encephalitozoon cuniculi infection of farmed foxes using an enzyme-linked immunosorbent assay. Meng, Xiangchun,Zheng, Jun,Gao, Yang,Jia, Honglin,Meng, Xiangchun,Zhang, Yanlong.

作者其他论文 更多>>