口蹄疫病毒非结构蛋白3AB基因的可溶性表达与反应原性的鉴定
文献类型: 中文期刊
作者: 祝秀梅;卢曾军;胡永浩;曹轶梅;马江涛;刘在新
作者机构:
关键词: 口蹄疫病毒;非结构蛋白3AB;ELISA
期刊名称:农业生物技术学报
ISSN: 1006-1304
年卷期: 2008 年 16 卷 01 期
页码: 15-19
收录情况: 北大核心 ; CSCD
摘要: 设计特异性引物扩增得到完整的口蹄疫病毒(FMDV)非结构蛋白(NSP)3AB基因,定向克隆到表达载体pET-30a中,获得了重组质粒pET-3AB,并转化大肠杆菌(Escherichia coli)BL21(DE3),重组菌分别在37℃和28℃条件下诱导表达,表达产物经SDS-PAGE电泳鉴定,观察到37℃培养条件下目的蛋白形成包涵体,在28℃条件下培养目的蛋白以可溶性的形式表达。Western blot分析表明,表达的目的蛋白能与FMDV感染牛血清发生特异性反应。以纯化的3AB蛋白作为抗原,采用间接ELISA模式检测了部分FMDV感染动物血清和健康动物血清,证明表达蛋白与FMDV感染血清有很好的反应而与健康动物血清无反应。
分类号: S852.65
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