数字农科院2.0

金钗石斛对MPTP诱导PD小鼠的神经保护作用及机制研究

文献类型： 中文期刊

作者： 王孟迪;魏山山;姜宁;Alberto Carlos Pires Dias;刘新民;王琼

作者机构：

关键词： 金钗石斛;帕金森;1-甲基-4-苯基-1;2;3;6-四氢吡啶;美多芭;小鼠

期刊名称：中国比较医学杂志

ISSN： 1671-7856

年卷期： 2022 年

页码：

收录情况： 北大核心（2020版） ; 科技核心（2020版）

摘要： 目的探究金钗石斛提取物（DendrobiumnobileLindl，DNL）在1-甲基-4-苯基-1，2，3，6-四氢吡啶（1-methyl-4-phenyl-1，2，3，6-tetrahydropyridine，MPTP）诱导的小鼠帕金森病（Parkinsondisease，PD）模型中的神经保护作用。方法将小鼠随机分为对照组、模型组，美多芭组（阳性药组）、DNL（100、200、300mg/kg）组，通过腹腔注射（i.p）MPTP（30mg/（kg·d），7d）建立亚急性小鼠PD模型，进行步态分析实验、爬杆实验、悬挂实验等评估DNL对PD小鼠运动异常的影响；酶联免疫吸附测定法（ELISA）检测各组小鼠纹状体区和皮层区肿瘤坏死因子-α（tumornecrosisfactor-α，TNF-α），白细胞介素-6（interleukin-6，IL-6）、和白细胞介素-1β（interleukin-1β，IL-1β）表达的变化；蛋白质印迹法（Westernblot）检测各组小鼠纹状体caspase-3和caspase-9的表达情况。结果模型组与对照组相比，推进指数、支撑时相的步态指标有显著变化（P＜0.05）；与模型组相比，美多芭组、DNL（100、200、300mg/kg）组在左后、右前、右后推进指数，左后、右前、右后支撑时相均有明显改善（P＜0.05）；与对照组相比，模型组在爬杆上半段和总时间增加（P<0.01、P<0.001），调头时间和下半段时间无明显变化（P＞0.05）；与模型组相比，DNL（300mg/kg）组可以改善上半段时间（P＜0.05），美多芭和DNL（100、200mg/kg）组无明显改善（P＞0.05）；与对照组相比，模型组的悬挂评分降低（P＜0.05），与模型组相比，DNL（300mg/kg）可以增加悬挂评分（P＜0.05），美多芭和DNL（100、200mg/kg）无明显改善（P＞0.05）；与对照组相比，模型组小鼠纹状体中TNF-α、IL-1β、IL-6含量增加（P＜0.05），caspase-3和caspase-9蛋白表达水平显著增高（P＜0.001）；与模型组相比，美多芭、DNL（300mg/kg）组炎症因子指标均降低（P＜0.05），皮层中各组含量无差异（P＞0.05）；caspase-3和caspase-9蛋白表达水平均降低（P＜0.05）。结论DNL能够改善MPTP诱导的PD小鼠行为变化，减轻脑组织中的炎症反应，抑制神经细胞凋亡，在治疗PD药物及功能食品研发方面具有较好的潜力。

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