数字农科院2.0

A亚群禽白血病病毒基因组m6A修饰在逃逸天然免疫反应的作用研究

文献类型： 中文期刊

作者： 李静蕾;于蒙蒙;陈运通;范文瑞;吴龙波;李雨萌;张子博;张艳萍;祁小乐;刘永振;王素艳;崔红玉;段雨路;李留安;高玉龙

作者机构：

关键词： A亚群禽白血病病毒;基因组RNA;m6A;β-干扰素

期刊名称：中国家禽

ISSN： 1004-6364

年卷期： 2026 年

页码：

收录情况： 北大核心（2023版） ; ; 科技核心（2024版） ; ; 农林核心（2024版）

摘要： 研究旨在探究A亚群禽白血病病毒（ALV-A）感染宿主后逃逸天然免疫反应的分子机制，重点关注病毒基因组m6A修饰在此过程中的作用。试验采用qPCR检测ALV-A(RAV-1株)感染宿主细胞72 h内β-干扰素（IFN-β）的表达水平，评估其诱导天然免疫反应的能力；利用m6A RNA甲基化测定试剂盒检测ALV-A基因组RNA是否存在m6A修饰；通过两种策略验证m6A修饰的功能：一是将野生型ALV-A感染过表达m6A清除蛋白FTO的DF-1细胞系；二是通过药物抑制m6A修饰获得m6A缺失的病毒，将m6A缺失病毒与野生型病毒分别接种DF-1细胞，比较IFN-β表达水平；将m6A缺失病毒的基因组RNA转染DF-1细胞，进一步通过qPCR检测IFN-β表达变化。结果显示：ALV-A感染仅诱导较低水平的IFN-β表达，且其基因组RNA中存在m6A修饰。与野生型病毒相比，m6A缺失的病毒感染DF-1细胞后诱导的IFN-β水平显著升高，为野生型的3.6倍，转染m6A缺失病毒基因组RNA同样能诱导更高水平的IFN-β表达。研究表明，ALV-A通过对其基因组RNA进行m6A修饰，抑制宿主天然免疫反应中IFN-β的高效表达，从而逃逸免疫识别。

分类号：

  • 相关文献

[1]口蹄疫病毒链特异性荧光定量RT-PCR检测方法的建立. 王洪梅,贾春涛,胡桂学,夏咸柱,于力. 2015

[2]牦牛β-干扰素基因的克隆与表达. 李玉萍,景志忠,蒲训,窦永喜,陈国华. 2006

[3]N6-methyladenosine mRNA methylation is important for the light response in soybean. Liya Zhang,Yanyan Zhang,Jun Liu,Hongyu Li,Bin Liu,Tao Zhao. 2023

[4]Transcriptome-wide analyses of RNA m6A methylation in hexaploid wheat reveal its roles in mRNA translation regulation. Huang, Tao,He, Wei-Jie,Li, Cheng,Zhang, Jing-Bo,Liao, Yu-Cai,Song, Bo,Yang, Peng. 2022

[5]Potential Target Analysis of Triptolide Based on Transcriptome-Wide m6A Methylome in Rheumatoid Arthritis. Fan D.,Liu B.,谷晓峰.,张倩.,Ye Q.,Xi X.,Xia Y.,Wang Q.,Wang Z.,Wang B.,Xu Y.,Xiao C.. 2022

[6]YTHDF2 promotes mitotic entry and is regulated by cell cycle mediators. 费启立. 2020

[7]The transcriptome-wide N6-methyladenosine (m6A) map profiling reveals the regulatory role of m6A in the yak ovary. Shaoke Guo,Xingdong Wang,Mengli Cao,Xiaoyun Wu,Lin Xiong,Pengjia Bao,Min Chu,Chunnian Liang,Ping Yan,Jie Pei,Xian Guo. 2022

[8]Newcastle disease virus activates methylation-related enzymes to reprogram m6A methylation in infected cells. Weifeng Yuan,Yuechi Hou,Qingyi Wang,Ting Lv,Jinlian Ren,Lei Fan,Juncheng Cai,Bin Xiang,Qiuyan Lin,Ming Liao,Chan Ding,Libin Chen,Tao Ren. 2023

[9]Hypoxia-induced ALKBH5 aggravates synovial aggression and inflammation in rheumatoid arthritis by regulating the m6A modification of CH25H. Danping Fan,Qishun Geng,Bailiang Wang,Xing Wang,Ya Xia,Liwen Yang,Qian Zhang,Tingting Deng,Yuan Xu,Hongyan Zhao,Bin Liu,Cheng Lu,Xiaofeng Gu,Cheng Xiao. 2024

[10]METTL3-mediated m6A methylation regulates ovarian cancer progression by recruiting myeloid-derived suppressor cells. Jinyong Wang,Dakai Ling,Lulin Shi,Huayun Li,Minhua Peng,Huihong Wen,Tao Liu,Ruifang Liang,Yongjian Lin,Laiyou Wei,Guangzhi Zhang,Shanze Chen. 2023

[11]TurboID-mediated proximity labeling for screening interacting proteins of FIP37 in Arabidopsis. Xiaofang Li,Yanping Wei,Qili Fei,Guilin Fu,Yu Gan,Chuanlin Shi. 2024

[12]The RNA binding protein EHD6 recruits the m6A reader YTH07 and sequesters OsCOL4 mRNA into phase-separated ribonucleoprotein condensates to promote rice flowering. Song Cui,Peizhe Song,Chaolong Wang,Saihua Chen,Benyuan Hao,Zhuang Xu,Liang Cai,Xu Chen,Shanshan Zhu,Xiangchao Gan,Hui Dong,Yuan Hu,Liang Zhou,Haigang Hou,Yunlu Tian,Xi Liu,Liangming Chen,Shijia Liu,Ling Jiang,Haiyang Wang,Guifang Jia,Shirong Zhou,Jianmin Wan. 2024

[13]RNA modifications in plant biotic interactions. Linhao Ge,Fuan Pan,Mingxuan Jia,Delphine M. Pott,Hao He,Hongying Shan,Rosa Lozano-Durán,Aiming Wang,Xueping Zhou,Fangfang Li. 2025

[14]Genome-wide identification of the m6A gene family and analysis of m6A methylome in alfalfa under drought stress. Xiang Meng,Dengxia Yi,Lin Ma,Xiaoran Ma,Kaiyun Xie,Xuemin Wang,Junbo Yang,Jun Tang. 2025

[15]Epi-transcriptome analysis reveals the lack of N6-methyladenosine modification in two RNA viruses infecting watermelon. 潘福安,,,,荆嘉贤,,,, Yanhong Qiu,,,,Maoying Li,,,,周雪平,,,,李少芳. 2025

作者其他论文 更多>>