数字农科院2.0

Generation and application of immortalized sheep fetal fibroblast cell line

文献类型： 外文期刊

作者： Guoyu Du;Cheng Zhang;Xiaoan Cao;Lingxia Li;Yong Zhang;Youjun Shang;Jinyan Wu

作者机构：

关键词： Application;Immortalized;ORFV vaccine strain;Sheep fetal fibroblasts;SV40 large T antigen

期刊名称： BMC Veterinary Research

ISSN： 1746-6148

年卷期： 2024 年 20 卷 1 期

页码：

收录情况： SCIE（2024版）

摘要： Background: Primary sheep fetal fibroblasts (SFFCs) have emerged as a valuable resource for investigating the molecular and pathogenic mechanisms of orf viruses (ORFV). However, their utilization is considerably restricted due to the exorbitant expenses associated with their isolation and culture, their abbreviated lifespan, and the laborious procedure. Results: In our investigation, the primary SFFCs were obtained and immortalized by introducing a lentiviral recombinant plasmid containing the large T antigen from simian virus 40 (SV40). The expression of fibronectin and vimentin proteins, activity of SV40 large T antigen, cell proliferation assays, and analysis of programmed cell death revealed that the immortalized large T antigen SFFCs (TSFFCs) maintained the same physiological characteristics and biological functions as the primary SFFCs. Moreover, TSFFCs demonstrated robust resistance to apoptosis, extended lifespan, and enhanced proliferative activity compared to primary SFFCs. Notably, the primary SFFCs did not undergo in vitro transformation or exhibit any indications of malignancy in nude mice. Furthermore, the immortalized TSFFCs displayed live ORFV vaccine susceptibility. Conclusions: Immortalized TSFFCs present valuable in vitro models for exploring the characteristics of ORFV using various techniques. This indicates their potential for secure utilization in future studies involving virus isolation, vaccine development, and drug screening.

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