数字农科院2.0

Chromosome-level genome assembly of the crofton weed (Ageratina adenophora)

文献类型： 外文期刊

作者： Fuyan Liu;Lin Du;Tingting Li;Bo Liu;Jianyang Guo;Guifen Zhang;Yibo Zhang;Wanxue Liu;Yan Pan;Ying Zhang;Hailing Wang;Ruiying Li;Weining Song;Fanghao Wan

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期刊名称： Scientific Data

ISSN： 2052-4463

年卷期： 2025 年 12 卷 1 期

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收录情况： SCIE（2025版）

摘要： Crofton weed (Ageratina adenophora), a significant invasive species, extensively disrupts ecosystem stability, leading to considerable economic losses. However, genetic insights into its invasive mechanisms have been limited by a lack of genomic data. In this study, we present the successful de novo assembly of the triploid genome of A. adenophora, leveraging long-read PacBio Sequel, optical mapping, and Hi-C sequencing. Our assembly resolved into a haplotype-resolved genome comprising 51 chromosomes, with a total size of ~3.82 Gb and a scaffold N50 of 70.8 Mb. BUSCO analysis confirmed the completeness of 97.71% of genes. Genome annotation revealed 3.16 Gb (76.44%) of repetitive sequences and predicted 123,134 protein-coding genes, with 99.03% functionally annotated. The high-quality reference genome will provide valuable genomic resources for future studies on the evolutionary dynamics and invasive adaptations of A. adenophora.

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