数字农科院2.0

Unveiling the development of cotton somatic embryos: Integrating spatial transcriptomics and metabolomics

文献类型： 外文期刊

作者： Aizhi Qin;Yaping Zhou;Xiaole Yu;Xiaoyang Ge;Zhixin Liu;Hao Liu;Lam Son Phan Tran;Xuwu Sun

作者机构：

关键词： Cotton;Somatic embryo;Spatial metabolome;Spatial transcriptome

期刊名称： Industrial Crops and Products

ISSN： 0926-6690

年卷期： 2025 年 230 卷

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： Plant development necessitates a diverse array of cell types, metabolites, and intricate gene regulation patterns. Consequently, investigating the spatial localization of cells within tissues, the spatial expression of genes, and the distribution of both primary and secondary metabolites in plants is crucial. The process of somatic embryo development is vital for the genetic enhancement of cotton, yet it remains poorly understood. Moreover, there is currently a scarcity of available data resources on the spatial expression patterns of genes and metabolites in cotton somatic embryo cells. In this study, we employed 10 × Visium spatial transcriptomics (ST) technology and air flow-assisted desorption electrospray ionization mass spectrometry imaging (AFADESI-MSI) spatial metabolomics (SM) technology to establish sequencing libraries and metabolomes of upland cotton (Gossypium hirsutum) somatic embryos, specifically focusing on globular, torpedo, and cotyledonary embryos. Specifically, we have highlighted the spatially regulated gene networks and metabolite gradients critical to embryogenesis. For instance, the integrated analysis of ST and SM revealed that polyamines (putrescine, spermidine, and spermine) and their biosynthesis-related genes (ADC, ODC, ARGAH1, SPDSYN1, and SPMS) were highly expressed in globular embryos. These findings suggest that polyamines and their biosynthesis-related genes may play a pivotal role in the conversion of embryogenic callus into somatic embryos in cotton. In summary, we propose an integrated spatially resolved multiomics approach, which will aid in deciphering the complex regulatory networks involved in the development of cotton somatic embryos.

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