数字农科院2.0

Metabolic Profiles Reveal Changes in the Leaves and Roots of Rapeseed (Brassica napus L.) Seedlings under Nitrogen Deficiency

文献类型： 外文期刊

作者： Shen, Xinjie;Yang, Ling;Han, Peipei;Gu, Chiming;Li, Yinshui;Liao, Xing;Qin, Lu

作者机构：

关键词： rapeseed;nitrogen deficiency;root architecture;metabolome;plant hormones

期刊名称： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

ISSN：

年卷期： 2022 年 23 卷 10 期

页码：

摘要： Rapeseed (Brassica napus L.) is an important oil crop species and plays a crucial role in supplying edible oil worldwide. However, rapeseed production in the field is often severely inhibited due to nitrogen (N) deficiency. Metabolites play key roles in plant growth and resistance to environmental stress, but little is known about the differential synthesis and accumulation of metabolites underlying rapeseed adaptation to N deficiency. Here, we studied the phenotypic response and used LC-electrospray ionization (ESI), ESI-MS/MS, and widely untargeted metabolomic approaches to detect differences in rapeseed under normal N (HN) and N-deficient (LN) conditions. The results showed that N deficiency severely inhibited rapeseed shoot growth and promoted rapeseed root architectural changes under LN conditions. In total, 574 metabolites were detected, and there were 175 and 166 differentially accumulated metabolites in the leaves and roots between the HN and LN conditions, respectively. The significantly differentially accumulated metabolites were involved in four primary metabolic pathways, namely, sucrose, phenylalanine, amino acid, and tricarboxylic acid cycle metabolism. Notably, we found that plant hormones have distinct accumulation patterns in rapeseed and coordinate to play crucial roles in both maintaining growth and protecting against damage from plant disease under HN and LN conditions. Moreover, our results indicated that flavonoid compounds, especially anthocyanins and rutin, may play important roles in increasing root cell resistance to oxidative damage and soil pathogen infections. Overall, this work provides valuable information for understanding the overall metabolite changes in rapeseed under N deficiency conditions, which may be beneficial for improving and producing new varieties of rapeseed capable of high yields under low N conditions.

分类号：

  • 相关文献

[1]Lipidomic and Metabolomic Analyses Reveal Changes of Lipid and Metabolite Profiles in Rapeseed during Nitrogen Deficiency. Peng, Yan,Lou, Hongxiang,Tan, Zengdong,Ouyang, Zhewen,Zhang, Yuting,Lu, Shaoping,Guo, Liang,Yang, Bao. 2023

[2]Integrated Transcriptome and Metabolome Analysis Reveals the Resistance Mechanisms of Brassica napus Against Xanthomonas campestris. Cong Zhou,Li Xu,Rong Zuo,Zetao Bai,Tongyu Fu,Lingyi Zeng,Li Qin,Xiong Zhang,Cuicui Shen,Fan Liu,Feng Gao,Meili Xie,Chaobo Tong,Li Ren,Junyan Huang,Lijiang Liu,Shengyi Liu. 2025

[3]Physiological, biochemical, and multi-omics analyses reveal the mechanism underlying strigolactone-mediated waterlogging response in rapeseed. Ximin Zhi,Xiaohua Bian,Yuexia Zhang,Yuxi Li,Aqarahim Wasim,Guangsheng Zhou,Chen Chen,Ni Ma. 2026

[4]城郊农区油菜立体套种模式研究(英文). 杨良金,夏晓进,张春雷,陶文才,杨鑫海,成海燕,尹金凤. 2007

[5]油菜栽培管理模拟优化决策系统试验示范(英文). 张春雷,曹洪鑫,李光明,李锋,赵懿. 2007

[6]中国的油菜生产与栽培科学技术研究(英文). 王汉中,官春云,张春雷. 2007

[7]Physiological and metabolic profiles of common reed provide insights into plant adaptation to low nitrogen conditions. Chu, Xiaodan,Yang, Lan,Wang, Shuang,Yang, Haijun,Liu, Jun.

[8]Leaf Volatile Compounds and Associated Gene Expression during Short-Term Nitrogen Deficient Treatments in Cucumis Seedlings. Deng, Jie,Yu, Hong-Jun,Li, Yun-Yun,Zhang, Xiao-Meng,Liu, Peng,Li, Qiang,Jiang, Wei-Jie,Jiang, Wei-Jie. 2016

[9]Effect of nitrogen deficiency on ascorbic acid biosynthesis and recycling pathway in cucumber seedlings. Zhang, Xue,Yu, Hong Jun,Zhang, Xiao Meng,Yang, Xue Yong,Li, Qiang,Jiang, Wei Jie,Jiang, Wei Jie,Zhao, Wen Chao.

[10]RNA-Seq-Based Transcriptome Profiling of Early Nitrogen Deficiency Response in Cucumber Seedlings Provides New Insight into the Putative Nitrogen Regulatory Network. Zhao, Wenchao,Yang, Xueyong,Yu, Hongjun,Jiang, Weijie,Sun, Na,Liu, Xiaoran,Liu, Xiaolin,Zhang, Xiaomeng,Wang, Yan,Gu, Xingfang,Zhao, Wenchao.

[11]Identification of differentially-expressed genes of rice in overlapping responses to bacterial infection by Xanthomonas oryzae pv. oryzae and nitrogen deficiency. Chen Hua-min,Tian Fang,He Chen-Yang,Bi Yong-Mei,Steven, Rothstein J.,Steven, Rothstein J.,Jan, Leach E.. 2015

[12]Combining nitrogen effects and metabolomics to reveal the response mechanisms to nitrogen stress and the potential for nitrogen reduction in maize. Yan li LU,Gui pei SONG,Yu hong WANG,Luo bin WANG,Meng ze XU,Li ping ZHOU,Lei WANG. 2023

[13]GmTDN1 improves wheat yields by inducing dual tolerance to both drought and low-N stress. Zhou, Yongbin,Liu, Jun,Guo, Jinkao,Wang, Yanxia,Ji, Hutai,Chu, Xiusheng,Xiao, Kai,Qi, Xueli,Hu, Lin,Li, Hui,Hu, Mengyun,Tang, Wensi,Yan, Jiji,Yan, Huishu,Bai, Xinxuan,Ge, Linhao,Lyu, Mingjie,Chen, Jun,Xu, Zhaoshi,Chen, Ming,Ma, Youzhi. 2022

[14]Foxtail millet [Setaria italica (L.) P. Beauv.] grown under nitrogen deficiency exhibits a lower folate contents. Wang Y.,Wang J.-S.,Dong E.-W.,Liu Q.-X.,Wang L.-G.,Chen E.-Y.,Jiao X.-Y.,Diao X.-M.. 2023

[15]Comparative Transcriptomic Analysis Reveals the Negative Response Mechanism of Peanut Root Morphology and Nitrate Assimilation to Nitrogen Deficiency. Lijie Li,Xiangguo Cheng,Xiangjun Kong,Peipei Jia,Xiaohui Wang,Lei Zhang,Xiaotian Zhang,Yi Zhang,Zhiyong Zhang,Baohong Zhang. 2023

[16]Metabolic, transcriptional, and hormonal responses of Panax ginseng C. A. Meyer to nitrogen deficiency. Hao Liang,Hai Sun,Cai Shao,Bochen Lv,Jiapeng Zhu,Weiyu Cao,Jixin Zhou,Yayu Zhang. 2025

[17]Overexpression of MdTGA1 enhances the adaptability to nitrogen and phosphorus deficiency in apple (Malus domestica Borkh.). Bin Xie,Xiuhong An,Sumiao Yang,Yanzhen Zhang,Xin Li,Jiangtao Zhou,Guodong Kang,Yanhui Chen,Cungang Cheng. 2025

[18]Genetic dissection of seminal root architecture in elite durum wheat germplasm. Sanguineti, M. C.,Li, S.,Maccaferri, M.,Corneti, S.,Rotondo, F.,Chiari, T.,Tuberosa, R.. 2007

[19]Characterization of root architecture in an applied core collection for phosphorus efficiency of soybean germplasm. Zhao, J,Fu, JB,Liao, H,He, Y,Nian, H,Hu, YM,Qiu, LJ,Dong, YS,Yan, XL.

[20]Overexpression of wheat gene TaMOR improves root system architecture and grain yield in Oryza sativa. Li, Bo,Liu, Dan,Li, Qiaoru,Mao, Xinguo,Li, Ang,Wang, Jingyi,Chang, Xiaoping,Jing, Ruilian.

作者其他论文 更多>>