数字农科院2.0

Lipidomics and transcriptomics profiling reveals a molecular regulatory network for lipids heterogeneity of intramuscular fat and subcutaneous fat in Fengjing pig

文献类型： 外文期刊

作者： Zhang, Yingying;Zhang, Jiajie;Wang, Hongyang;Tu, Weilong;Diao, Yuduan;Pei, Huijie;Huang, Ji;Zhou, Jieke;Song, Xin;Wang, Ligang;Tan, Yongsong

作者机构：

关键词： Meat quality;Lipid profiles;RNA-Seq;Intramuscular fat;Subcutaneous fat;Fengjing pig

期刊名称： FOOD BIOSCIENCE

ISSN： 2212-4292

年卷期： 2025 年 75 卷

页码：

收录情况： SCIE（2025版） ; ; 农林核心（2024版）

摘要： Intramuscular fat (IMF) and subcutaneous adipose fat (SAF), located in distinct anatomical regions, play a crucial role in determining the quality and flavor of pork. However, the molecular mechanisms underlying the heterogeneity in lipid properties between IMF and SAF remain poorly. This research investigates the lipid profiles of the longissimus thoracis (LT) and SAF in Fengjing pigs through non-targeted lipidomics and transcriptomics. We identified 1204 lipid molecules that exhibited significant differences, alongside 2805 genes that were markedly upregulated and 3134 genes that were notably downregulated in LT compared to SAF. Functional enrichment analysis of the differentially expressed genes (DEGs) and lipids revealed significant enrichment in the glycerophospholipids (GPs) metabolism pathway, leading to the construction of a molecular regulatory network links DEGs and lipids associated with GPs metabolism. Through orthogonal Partial Least Squares Discriminant Analysis (OPLS-DA) and redundancy analysis (RDA), we identified a strong correlation among 12 key DEGs and 9 critical lipids. This study provides novel insights into the gene-lipid relationship, enhancing our understanding of the differences in lipogenesis and lipolysis between LT and SAF, which may inform future efforts to improve the meat quality and flavor of Fengjing pigs.

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