数字农科院2.0

Overexpression of MdTGA1 enhances the adaptability to nitrogen and phosphorus deficiency in apple (Malus domestica Borkh.)

文献类型： 外文期刊

作者： Bin Xie;Xiuhong An;Sumiao Yang;Yanzhen Zhang;Xin Li;Jiangtao Zhou;Guodong Kang;Yanhui Chen;Cungang Cheng

作者机构：

关键词： Apple (Malus domestica borkh.) rootstock;Nitrogen deficiency;Nutrient use efficiency;Phosphorus deficiency;Transcription factor

期刊名称： Plant Physiology and Biochemistry

ISSN： 0981-9428

年卷期： 2025 年 229 卷

页码：

收录情况： SCIE（2025版）

摘要： High quality and yield in apple (Malus domestica Borkh.) require adequate nitrogen (N) and phosphorus (P) nutrients. To address N and P deficiencies in soil and the excessive application of chemistry fertilizers in production, it is critical to explore regulators for efficient N-P coordinated utilization. In this study, we observed that the transcription factor MdTGA1 was induced by both nitrate (NO3−) and inorganic phosphate (Pi) deficiency. Further, overexpression of MdTGA1 positively regulated the tolerance of apple to N and P deficiencies. This was observed in the greater leaf photochemical efficiency, height, fresh weight and root volume in transgenic plants compared to wild-type under N deficient and P deficient conditions. Moreover, overexpression of MdTGA1 in plants improved the transcription level of MdNRT1.1, MdNRT2.1 and MdNRT2.4 in the roots, and remarkably increased NO3− influx on the root surfaces, enhanced NO3− nitration and ammonium assimilation processes, remarkably increased the content of NO3−, total N, 15N and amino acids. Furthermore, overexpression of MdTGA1 had positive effects on the expression of P homeostasis-regulated gene MdSPXs, remarkably up-regulated several Pi transporter genes, such as MdPHT1;3, MdPHT1;4, MdPHO1;7, and MdPHO1;9, which were beneficial for Pi absorption and transport in apple plants, enhanced the acid phosphatase activity, and increased total P accumulation in plants. Overall, MdTGA1 serves as the key integrator for NO3− and Pi signalling crosstalk in apple. Therefore, this study provides candidate genes for the study of N-P signaling network interactions as well as the creation of N- and P-efficient apple rootstock germplas. It lays a theoretical basis for research into reducing fertilizer use and increasing productivity in the apple industry.

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