数字农科院2.0

Diagnostic Efficacy of Plasma-Based Real-Time PCR for Schistosomiasis Japonica in Mice before and after Treatment with Praziquantel

文献类型： 外文期刊

作者： Cheng Chen;Xue Zhou;Qinghong Guo;Chao Lv;Yalan Tang;Qingqing Guo;Yang Chen;Kerou Zhou;Zhiqiang Fu;Jinming Liu;Jiaojiao Lin;Yang Hong;Jun Hu Chen

作者机构：

关键词： efficacy evaluation;praziquantel;real-time fluorescent quantitative PCR;Schistosoma japonicum

期刊名称： Animals

ISSN： 2076-2615

年卷期： 2023 年 13 卷 19 期

页码：

收录情况： SCIE（2023版）

摘要： The prevalence of schistosomiasis japonica in China is now characterized by a low epidemic rate and low-intensity infections. Some diagnostic methods with high sensitivity and specificity are urgently needed to better monitor this disease in the current situation. In this study, the detection efficacy of a real-time fluorescent quantitative PCR (qPCR) assay was assessed for schistosomiasis japonica in mice, and before and after treatment with praziquantel (PZQ). Our results showed that the sensitivity of the qPCR was 99.3% (152/153, 95% CI: 96.41–99.98%) and its specificity was 100% (77/77, 95% CI: 95.32–100%) in mice infected with different numbers of Schistosoma japonicum. After the oral administration of PZQ, mice infected with 10 cercariae or 40 cercariae were all Schistosoma japonicum-negative 6 weeks after treatment. However, the negativity rates on a soluble egg antigen (SEA)-based enzyme-linked immunosorbent assay (ELISA) were only 34.8% (8/23, 10 cercariae group) and 6.7% (1/15, 40 cercariae group) at the sixth week after PZQ treatment. These results demonstrated that the qPCR method had good sensitivity and specificity, and suggested that its sensitivity correlated with the infection intensity in mice. Moreover, this method had better potential utility for evaluating the treatment efficacy of PZQ in schistosome-infected mice than SEA-based ELISA.

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