数字农科院2.0

Synergistic modification of sunflower seed protein by pH-cycling and ultrasound to improve its structural and functional properties

文献类型： 外文期刊

作者： Zhenyuan Li;Xuegang Huang;Jiaxin Guo;Yumeng Hu;Karim Gafurov;Jinchuang Zhang;Qiang Wang;Qin Guo

作者机构：

关键词： pH-cycling;Solubility;Structural modification;Sunflower seed protein;Ultrasound

期刊名称： Food Hydrocolloids

ISSN： 0268-005X

年卷期： 2025 年 172 卷

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： Sunflower seed protein (SPI) is a high-quality plant-based protein with balanced nutrition, low allergenicity, and excellent sustainability. However, its limited solubility hinders the full expression of its functional properties, thereby restricting its broader application in food processing. To address this issue, SPI was modified via a synergistic treatment combining pH-cycling and ultrasound in this study, and its modification mechanism and functional properties were comprehensively evaluated. The results indicate that the combined treatment induced protein structural aggregation-disaggregation-rearrangement, significantly enhancing SPI solubility to 81.27 %, representing a 58.14 % increase compared to unmodified protein. During modification, pH-cycling treatment converted SPI molecules into a molten globule state, while ultrasonic cavitation and shear forces effectively disrupted protein aggregates. This led to increased exposure of chromophores on side chains, enhanced fluorescence intensity, reduced particle size to 81.08 nm, and increased ζ-potential (−38.77 mV), thereby improving system stability. The modified SPI exhibited a higher enthalpy change (ΔH = 3.58 J/g), along with markedly improved functional characteristics: foaming capacity and foam stability increased by 2.29 times and 2.69 times, respectively; emulsifying activity and stability reached 6.23 m2/g and 88.72 %; and oil-holding capacity was enhanced to 5.52 g/g, representing 2.73 times increase. Overall, the synergistic pH-cycling and ultrasound modification proved highly effective in improving the solubility and functional performance of sunflower seed protein, offering promising potential for its application in advanced food systems.

分类号：

  • 相关文献

[1]Sunflower seed protein: defatting, structural characterization and property analysis. Zhenyuan Li,Xuegang Huang,Yumeng Hu,Sarina Ma,Karim Gafurov,Fengying Gu,Qin Guo,Qiang Wang. 2025

[2]Application of transglutaminase modifications for improving protein fibrous structures from different sources by high-moisture extruding. Jinchuang Zhang,Tongqing Li,Qiongling Chen,Haodong Liu,David L. Kaplan,Qiang Wang. 2023

[3]Updates on the chemistry, processing characteristics, and utilization of tea flavonoids in last two decades (2001-2021). Shi, Jiang,Yang, Gaozhong,You, Qiushuang,Sun, Shili,Chen, Ruohong,Lin, Zhi,Simal-Gandara, Jesus,Lv, Haipeng. 2022

[4]An updated review of isoquinoline alkaloids: Biological activity and mode of action, structural modifications, and agricultural applications. Xiaorong Yang,Tong Bu,Yuchao Ma,Xiaoyan Yu,Zile Gong,Jing Wang,Xueyan Liu,Janar Jenis,Hongwei Hu,Xiaolou Miao,Xiaofei Shang. 2025

[5]Functional Characteristics of Milk Protein Concentrates and Their Modification. Uluko, Hankie,Liu, Lu,Lv, Jia-Ping,Zhang, Shu-Wen,Uluko, Hankie.

[6]Tangeretin-loaded protein nanoparticles fabricated from zein/beta-lactoglobulin: Preparation, characterization, and functional performance. Chen, Jingjing,Zheng, Jinkai,McClements, David Julian,Xiao, Hang,Zheng, Jinkai. 2014

[7]The Amino Acid Composition, Solubility And Emulsifying Properties Of Sweet Potato Protein. Mu, Tai-Hua,Tan, Sze-Sze,Xue, You-Lin.

[8]Complexation of synthetic CDM-AM copolymer with natamycin and carbendazim to improve solubility and fungicidal activity. Li, Yong-Fu,Ji, Jing,Guo, Qin,Ha, Yi-Ming,Li, Qing-Peng,Li, Yong-Fu,Ji, Jing,Guo, Qin,Ha, Yi-Ming,Li, Qing-Peng.

[9]Physicochemical characterization of sweet potato starches popularly used in Chinese starch industry. Abegunde, Oluwaseyi Kemi,Mu, Tai-Hua,Chen, Jing-Wang,Deng, Fu-Ming.

[10]Characterization of recombinant plectasin: Solubility, antimicrobial activity and factors that affect its activity. Yang, Yalin,Teng, Da,Zhang, Jun,Tian, Zigang,Wang, Shaoran,Wang, Jianhua,Yang, Yalin,Teng, Da,Zhang, Jun,Tian, Zigang,Wang, Shaoran,Wang, Jianhua. 2011

[11]Optimization of extraction conditions for improving solubility of peanut protein concentrates by response surface methodology. Ma, Tiezheng,Wang, Qiang,Wu, Haiwen. 2010

[12]Nano-EMB-SP improves the solubility, foliar affinity, photostability and bioactivity of emamectin benzoate. Gao, Fei,Cui, Bo,Wang, Chong,Li, Xingye,Li, Bohao,Zhan, Shenshan,Shen, Yue,Zhao, Xiang,Sun, Changjiao,Wang, Chunxin,Wang, Yan,Zeng, Zhanghua,Cui, Haixin. 2022

[13]Effect of high-moisture extrusion and addition of transglutaminase on major peanut allergens content extracted by three step sequential method. Shah Faisal,Jinchuang Zhang,Shi Meng,Aimin Shi,Liu Li,Qiang Wang,Soheila J. Maleki,Benu Adhikari. 2022

[14]A Novel Process for One-Step Separation and Cyclodextrin Inclusion of Ginsenoside Rg5 from Ginseng Stem–Leaf Saponins (GSLS): Preparation, Characterization, and Evaluation of Storage Stability and Bioactivity. Jianbo Chen,Meijia Li,Xiaohui Huo,Zhiman Li,Di Qu,Jiyue Sha,Yinshi Sun. 2023

[15]Polymers Enhance Chlortetracycline Hydrochloride Solubility. Chao Zhang,Bing Li,Yubin Bai,Yangling Liu,Yong Zhang,Jiyu Zhang. 2024

[16]TBAPy-based metal-organic frameworks with phosphate-induced fluorescence for detecting gossypol. Li, Jiaxing,Sun, Yuanqiang,Li, Zhaohui,Yang, Ran,Qu, Lingbo. 2024

[17]The pH-driven binding mechanism of rice glutelin-dipotassium glycyrrhizinate and its effects on protein solubility and aggregation behavior. Li, Ning,Wang, Fengzhang,Li, Yang,Li, Dezhi,Wang, Huanhuan,Shan, Yimeng,Nie, Mengzi,Wang, Jing,Tong, Li-Tao. 2025

[18]Effects of microwave and ultrasound pretreatments on enzymolysis of milk protein concentrate with different enzymes. Uluko, Hankie,Zhang, Shuwen,Liu, Lu,Chen, Jianhang,Sun, Yanjun,Su, Yanling,Li, Hongjuan,Cui, Wenming,Lv, Jiaping,Uluko, Hankie. 2013

[19]Response surface optimization of angiotensin converting enzyme inhibition of milk protein concentrate hydrolysates in vitro after ultrasound pretreatment. Uluko, Hankie,Li, Hongjuan,Cui, Wenming,Zhang, Shuwen,Liu, Lu,Chen, Jianhang,Sun, Yanjun,Su, Yanling,Lv, Jiaping,Uluko, Hankie. 2013

[20]Pilot-scale membrane fractionation of ACE inhibitory and antioxidative peptides from ultrasound pretreated milk protein concentrate hydrolysates. Uluko, Hankie,Zhang, Shuwen,Liu, Lu,Li, Hongjuan,Cui, Wenming,Xue, Haixiao,Zhao, Lili,Lu, Jing,Lv, Jiaping,Uluko, Hankie,Sun, Yanjun. 2014

作者其他论文 更多>>