数字农科院2.0

ZmGluTR1 is involved in chlorophyll biosynthesis and is essential for maize development

文献类型： 外文期刊

作者： Wenzhu Yang;Yuhan Yuan;Pengjuan Yang;Suzhen Li;Shuai Ma;Xiaoqing Liu;Xiaojin Zhou;Rumei Chen

作者机构：

关键词： Binding protein;Chlorophyll synthesis pathway;Glutamyl-tRNA reductase;Maize;Protein interaction

期刊名称： Journal of Plant Physiology

ISSN： 0176-1617

年卷期： 2023 年 290 卷

页码：

收录情况： SCIE（2023版）

摘要： Chlorophyll is the most important carrier of photosynthesis in plants and is therefore vital for plant growth and development. Synthesis of 5-aminolevulinic acid (ALA) is initiated and catalyzed by glutamyl-tRNA reductase (GluTR) and is the rate-limiting step in chlorophyll biosynthesis. GluTR is controlled by several regulating factors. Although many studies have investigated the structure and function of GluTR in plants, the maize (Zea mays L.) GluTR has not yet been reported. Here, we isolated and identified the first loss-of-function mutant of GluTR in plants from a maize mutagenic population. The stop-gain mutation in ZmGluTR1 resulted in leaf etiolation throughout the growing season. The level of intermediates of chlorophyll biosynthesis and photosynthetic pigments decreased markedly and abnormal chloroplast structure was also observed in the mutants. Further analysis revealed that the deletion of carboxyl terminal (C-terminal) led to premature transcription termination and this hindered the interaction with FLUORESCENT (FLU), thereby influencing the stability of mutated ZmGluTR1 and leading to abolish interaction with GluTR-binding protein (GluBP). Moreover, mutations in the catalytic domain or nicotinamide adenine dinucleotide phosphate (NADPH) binding domain were lethal under normal growth conditions. These results indicate that ZmGluTR1 plays a fundamental role in chlorophyll biosynthesis and maize development.

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