数字农科院2.0

Improvement of amphipathic properties with molecular structure unfolding and activation of cottonseed protein as ultra stable and safe emulsifier by deamidation

文献类型： 外文期刊

作者： Kwame Eduam Baiden Frempong;Guiqiang He;Meng Kuang;Peng Jun;Min Xue;Yanxia Wei;Jian Zhou

作者机构：

关键词： Amphipathic;Cottonseed protein;Deamidation;Emulsifier;Hydrolysate

期刊名称： International Journal of Biological Macromolecules

ISSN： 0141-8130

年卷期： 2023 年 247 卷

页码：

收录情况： SCIE（2023版）

摘要： By-product cottonseed proteins are excellent options for numerous applications due to their superior properties and lower cost. However, its complex folded structure and large molecular weight lead to lower reactivity and insufficient amphiphilicity. Cottonseed protein isolate (CPI) is less-soluble in water. Therefore, we improved the amphiphilicity of CPI with associated hydrolysis, molecular structure unfolding, and activation by alkaline-induced deamidation (at 24, 36, and 72 h) and produced three cottonseed protein hydrolysates CPH 24, 36, and 72. FTIR/UV-CD measurements confirmed the conformational changes and conversion of the structural content. Particle size decreased 2503.4–771.8 nm, while surface hydrophobicity (133.5–326.7), carboxyl content (1.13 × 10־3–2.09 × 10־3), and flexibility increased, signifying hydrolysis, unfolding, and amphiphilicity improvement. Longer deamidation (CPH 72) exhibited the best properties, its prepared emulsions were long-term stable under all the environmental stresses without visible phase separation after at least 40 days of storage except at pH 4. Compared to CPI, it had smaller droplets (939.3–264.9 nm) and larger absolute ζ-potential (−26.5 to −58.0 mV). From the in-vitro cytotoxicity test, deamidated CPI is extremely safer than commonly used synthetic surfactants. This research provides a new method for producing multifunctional emulsifiers from CPI, which could be utilized in the development of functional foods/non-foods.

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