数字农科院2.0

Microfluidic-assisted integrated nucleic acid test strips for POCT

文献类型： 外文期刊

作者： Wang, Nan;Zhang, Juan;Xiao, Bin;Chen, Ailiang

作者机构：

关键词： Nucleic acid lateral flow assay;Test strips;Post-of-care testing;Rapid detection;Signal amplification

期刊名称： TALANTA

ISSN： 0039-9140

年卷期： 2024 年 267 卷

页码：

收录情况： SCIE（2024版） ; ; EI（2024版）

摘要： Numerous diseases have posed significant threats to public health, notably the global pandemic of COVID-19, resulting in widespread devastation due to its high infectivity and severity. The nucleic acid lateral flow assay (NALFA) addresses challenges of complexity, cost, and time associated with traditional assays, offering a reliable platform for rapid and precise nucleic acid target detection. NALFA is gaining prominence as a point-of-care testing (POCT) technique, thanks to its user-friendly operation and rapid results. Nevertheless, conventional NALFA relies on specialized technicians and involves labor-intensive steps like DNA extraction and PCR processes, impeding its efficiency. To overcome these limitations, integrating NALFA with microfluidic technology, widely employed in rapid field detection, holds promise. This review comprehensively outlines prevailing strategies for integrating NALFA, encompassing both research initiatives and commercial applications. Addressing the bottleneck of nucleic acid amplification as a rate-limiting step, the review delves into progress in amplification-free NALFA and highlights prevalent signal amplification techniques. Ultimately, the review outlines the future prospect of integrated NALFA development, capturing the technology's evolution and providing valuable insights for academic and commercial endeavors.

分类号：

  • 相关文献

[1]Accelerated Hybridization Chain Reaction Kinetics Using Poly DNA Tetrahedrons and Its Application in Detection of Aflatoxin B1. Xuesong Li,Jie Cheng,Kun Zeng,Shulin Wei,Jiaxuan Xiao,Yanyan Lu,Fang Zhu,Zhanhui Wang,Kun Wang,Xiangyang Wu,Zhen Zhang. 2023

[2]Entropy-driven multicolor DNA nanoflowers for simultaneous and rapid detection of multiple heavy metal ions in water. Wang, Jin,Wang, Yue,Lu, Yanyan,Wei, Shulin,Zhu, Nuanfei,Selvan, Govindaraj Tamil,Li, Yanshen,Ravikumar, Ayyanuservai,Zhang, Zhen. 2024

[3]Sensitive detection of multiple mycotoxins by SPRi with gold nanoparticles as signal amplification tags. Hu, Weihua,Chen, Hongming,Zhang, Hilanhuan,He, Guangli,Li, Xin,Li, Chang Ming,Hu, Weihua,Chen, Hongming,Zhang, Hilanhuan,He, Guangli,Li, Xin,Li, Chang Ming,Hu, Weihua,Chen, Hongming,Zhang, Hilanhuan,He, Guangli,Li, Xin,Li, Chang Ming,Hu, Weihua,Zhang, Xiaoxing,Liu, Yang.

[4]Conjugated cationic polymer-assisted amplified fluorescent biosensor for protein detection via terminal protection of small molecule-linked DNA and graphene oxide. Zhang, Zhen,Xia, Xiaoyang,Xiang, Xia,Huang, Fenghong,Han, Ling.

[5]Design of nuclease-based target recycling signal amplification in aptasensors. Yan, Mengmeng,Bai, Wenhui,Zhu, Chao,Huang, Yafei,Yan, Jiao,Chen, Ailiang,Yan, Mengmeng,Bai, Wenhui,Zhu, Chao,Huang, Yafei,Yan, Jiao,Chen, Ailiang,Huang, Yafei,Yan, Jiao.

[6]Ultrasensitive detection assay for Cronobacter sakazakii based on nucleic acid-driven aggregation-induced emission of gold nanoclusters and cascaded signal amplification. Qing Kang,Shuai qi Zhang,Tong Lin,Jin zhao Li,Chen jing Ma,Jing bo Jiao,Cheng Li,Xin jun Du,Shuo Wang. 2024

[7]Evaluation of a loop-mediated isothermal amplification assay based on hrpZ gene for rapid detection and identification of Pseudomonas syringae pv. lachrymans in cucumber leaves. Meng, X. -L.,Xie, X. -W.,Shi, Y. -X.,Chai, A. -L.,Li, B. -J.,Meng, X. -L.,Ma, Z. -H..

[8]Development and preliminary application of an immunochromatographic strip for rapid detection of infection with porcine reproductive and respiratory syndrome virus in swine. Li, Xue-song,Fu, Fang,Lang, Yue-kun,Li, Hai-zhong,Wang, Wei,Chen, Xin,Tian, Hua-bin,Li, Xi,Li, Xue-song,Zhou, Yan-jun,Tong, Guang-zhi. 2011

[9]Rapid detection of Betacoronavirus 1 from clinical fecal specimens by a novel reverse transcription loop-mediated isothermal amplification assay. Qiao, Jun,Meng, Qingling,Chen, Chuangfu,Zhang, Zaichao,Tian, Zhenzhong,Cai, Xuepeng. 2012

[10]Rapid detection of Akabane virus by a novel reverse transcription loop-mediated isothermal amplification assay (RT-LAMP). Qiao, Jun,Wang, Junwei,Meng, Qingling,Wang, Guochao,Liu, Yucheng,He, Zhihao,Yang, Haibo,Zhang, Zaichao,Chen, Chuangfu,Meng, Qingling,Cai, Xuepeng. 2013

[11]Gold nanoparticle-based colorimetric aptasensor for rapid detection of six organophosphorous pesticides. Bai, Wenhui,Zhu, Chao,Liu, Jinchuan,Yan, Mengmeng,Yang, Shuming,Chen, Ailiang,Bai, Wenhui,Zhu, Chao,Liu, Jinchuan,Yan, Mengmeng,Yang, Shuming,Chen, Ailiang. 2015

[12]Research on optic biosensor for rapid detection of hemoglobin. Baoshan He,Aiyu Zhou,Weiwei Yue,Liying Jiang,Chunxiu Liu,Xinxia Cai,Baoshan He,Aiyu Zhou,Weiwei Yue,Liying Jiang,Chunxiu Liu,Xinxia Cai. 2006

[13]A novel rapid detection of Senecavirus A using recombinase polymerase amplification (RPA) coupled with lateral flow (LF) dipstrip. Huibao Wang,Jinjie Dong,Tao Zhang,Fan Wang,Rui Yang,Yong Zhang,Xingxu Zhao. 2022

[14]Enzyme inhibition methods based on Au nanomaterials for rapid detection of organophosphorus pesticides in agricultural and environmental samples: A review. Rongqi Zhai,Ge Chen,Guangyang Liu,Xiaodong Huang,Xiao Min Xu,Lingyun Li,Yanguo Zhang,Jing Wang,Maojun Jin,Donghui Xu,A. M. Abd El-Aty. 2022

[15]CRISPR/Cas12a-mediated Enzymatic recombinase amplification for rapid visual quantitative authentication of halal food. Xiaohui Wang,Wenyu Jin,Yao Yang,Huizi Ma,Honghong Liu,Jiawen Lei,Yuhua Wu,Li Zhang. 2023

[16]Development of a real-time recombinase-aided amplification (RT-RAA) molecular diagnosis assay for sensitive and rapid detection of Toxoplasma gondii. Zhao Hua Wang,Wei Zhang,Xiu Zhong Zhang,Xin Ran Yao,Wei Huang,Hong Jia,Xiao Lei Liu,Shao Hua Hou,Xiao Jia Wang. 2021

[17]A Novel Immunochromatographic Strip Based on Latex Microspheres for the Rapid Detection of North American-Type Porcine Reproductive and Respiratory Syndrome Virus. Li, Wansheng,Li, Minhua,Zhang, Hongliang,Li, Chao,Xu, Hu,Gong, Bangjun,Fu, Jun,Guo, Zhenyang,Peng, Jinmei,Zhou, Guohui,Tian, Zhijun,Wang, Qian. 2022

[18]Rapid Nondestructive Prediction of Multiple Quality Attributes for Different Commercial Meat Cut Types Using Optical System. An, Jiangying,Li, Yanlei,Zhang, Chunzhi,Zhang, Dequan. 2022

[19]Development of a Real-Time Quantitative PCR Assay for the Specific Detection of Bacillus velezensis and Its Application in the Study of Colonization Ability. Xu, Shuai,Xie, Xuewen,Shi, Yanxia,Chai, Ali,Li, Baoju,Li, Lei. 2022

[20]A novel and low-cost cross-priming amplification assay for rapid detection of Babesia duncani infection. Yueli Nian,Shangdi Zhang,Jinming Wang,Xiaoyun Li,Yanbo Wang,Junlong Liu,Zeen Liu,Yuxin Ye,Chongge You,Hong Yin,Guiquan Guan. 2024

作者其他论文 更多>>