数字农科院2.0

Identification of two novel linear B-cell epitopes in p27 of feline leukemia virus using monoclonal antibodies

文献类型： 外文期刊

作者： Shiqiang Zhu;Aoxing Tang;Yingqi Zhu;Da Zhang;Meng Zhu;Miao Zhang;Na Li;Shuaisai Pang;Qi Zhang;Xiaomei Tan;Jie Zhu;Chuanfeng Li;Chunchun Meng;Guangqing Liu

作者机构：

关键词： B-cell epitope;FeLV p27;Monoclonal antibodies

期刊名称： International Journal of Biological Macromolecules

ISSN： 0141-8130

年卷期： 2025 年 333 卷

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： Feline leukemia virus (FeLV) is a gammaretrovirus that is prevalent among a wide range of felids worldwide and causes various hematopoietic disorders in domestic cats. The core protein p27 is the group-specific antigen of FeLV. It is soluble and abundantly produced, making it an ideal target for detection by immunoassays. In this study, the FeLV p27 gene, with a size of 744 bp, was expressed through prokaryotic expression systems, and the purified products were injected into BALB/c mice. Two new hybridoma cells (named mAb 1A6 and 3A7) were screened by Enzyme-Linked Immunosorbent Assay (ELISA), western blot analysis (WB), and indirect immunofluorescence assay (IFA). The antigenic epitope of these monoclonal antibodies was examined by WB or ELISA using a series of truncated GST-fused or artificially synthesized peptides. Further identification of monoclonal antibody target sequences revealed 42SILVTHQ48 (aa42–48) and 110AGREHL115 (aa110–115) as B-cell linear epitopes. An accurate identification of the epitope can provide important antigenic information, a prerequisite for effective detection. These results provided insights into the structure, function, and antigenicity of the p27 protein, thereby establishing the foundation for the development of diagnostic technologies and vaccine design for FeLV.

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