数字农科院2.0

An improved assay for detection of Acidovorax citrulli in watermelon and melon seed

文献类型： 外文期刊

作者： Zhao, T.;Feng, J.;Li, J.;Randhawa, P.

作者机构：

关键词： Methods and Techniques;Infection;Horticulture: Agriculture

期刊名称： SEED SCIENCE AND TECHNOLOGY

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Acidovorax citrulli (Ac), the causal agent of a bacterial seedling blight and fruit blotch, has emerged as a serious seedborne pathogen of watermelon and melons world-wide. Although attempts have been made to develop a simple routine laboratory seed assay to detect the organism in seeds, none is routinely used. Seeds contaminated with Ac remain a problem for the seed industry. We describe a combined agar plating, real-time PCR assay for detection of Ac in watermelon and melon seeds. The bacteria were extracted by soaking seeds in buffer containing vancomycin and assayed by dilution plating onto semiselective ethanol bromcresol purple /brilliant blue R (EBB) agar and onto EBB agar containing ampicillin (EBBA), by direct real-time PCR. and by real-time BIO-PCR (enrichment PCR). Using extracts of 1,000 healthy seeds spiked with a pure culture suspension of cells of Ac, the BIO-PCR assay using EBBA agar detected Ac in extracts containing as few as one cell per ml. When replicates of 1,000 healthy seeds were spiked with a single naturally infected seed, all the laboratory assays, including plating onto EBB and EBBA agar, direct PCR, and BIO-PCR were positive. This combined agar plating, real-time PCR protocol should prove useful as a routine assay for detection of Ac in watermelon and melon seeds, even in those samples containing relatively high levels of saprophytic bacteria.

分类号： S5

  • 相关文献

[1]Development of reverse-transcription loop-mediated isothermal amplification for the detection of infectious bursal disease virus. Xu, Jiangtao,Yin, Yanbo,Xu, Shouzhen,Wang, Jianlin,Liu, Xingcai,Zhang, Zhenmei,Cui, Shangjin,Guo, Yanyan,Li, Jida,Han, Limin.

[2]Generation and efficacy evaluation of a recombinant adenovirus expressing the E2 protein of classical swine fever virus. Sun, Yuan,Liu, Da-Fei,Wang, Yu-Fei,Liang, Bing-Bing,Cheng, Dan,Li, Na,Qi, Qiao-Fen,Zhu, Qing-Hu,Qiu, Hua-Ji.

[3]Population Dynamics of Sesamia inferens on Transgenic Rice Expressing Cry1Ac and CpTI in Southern China. Wu, Kongming,Peng, Yufa,Liu, Peilei,Wang, Feng.

[4]Molecular Cloning and Expression of cDNA Encoding the Cysteine Proteinase Inhibitor from Upland Cotton. Jiang, Ming-feng,Li, Sheng-wei,Chen, Min,Li, Sheng-wei,Chen, Min,Cai, Ying-fan,Xie, Yong-fang,Li, Biao,Sun, Quan,Jiang, Huai-zhong,Pan, Zheng,Gao, Yun-ling,Yuan, You-Lu,Shi, Yu-zheng.

[5]Influence of docosahexaenoic acid on the concentration of fatty acids and volatile fatty acids in rumen fluid analysed by a rumen-simulation techniques. Liu, L.,Wang, J. Q.,Bu, D. P.,Liu, S. J.,Liang, S.,Liu, K. L.,Wei, H. Y.,Zhou, L. Y..

[6]Ri-mediated Transformation of Glycyrrhiza uralensis with a Squalene Synthase Gene (GuSQS1) for Production of Glycyrrhizin. Lu, Hong-Yu,Zhang, Hai-Chao,Gao, Shan-Lin,Liu, Jing-Mei,Yin, Tao.

[7]A backward propagation neural network for predicting daily transpiration of poplar. Meijun, Yan,Peiling, Yang,Shumei, Ren,Yuanpei, Luo,Tingwu, Xu.

[8]Development of SNP markers and haplotype analysis of the candidate gene for rhg1, which confers resistance to soybean cyst nematode in soybean. Li, Ying-Hui,Zhang, Chen,Ma, Zulu,Liu, Zhang-Xiong,Nan, Hai-Yang,Chang, Ru-Zhen,Qiu, Li-Juan,Gao, Zhong-Shan,Smulders, Marinus Johannes Maria,Ma, Zulu.

[9]Determination of Mepiquat Chloride Residues in Cotton and Soil by Liquid Chromatography/Mass Spectrometry. Dong, Feng-Shou,Li, Shuo,Zheng, Yong-Quan.

[10]Field-Testing of Synthetic Herbivore-Induced Plant Volatiles as Attractants for Beneficial Insects. Zhang, Yongjun,Wu, Kongming,Guo, Yu Yuan,Yu, Huilin.

[11]Identification of Heterodera glycines using PCR with sequence characterised amplified region (SCAR) primers. Peng, Deliang,Ou, Shiqi,Liu, Xuemin,Li, Yu,Moens, Maurice,Moens, Maurice.

[12]The development and evaluation of a loop-mediated isothermal amplification (LAMP) method for detection of Babesia spp. infective to sheep and goats in China. Guan, Guiquan,Luo, Jianxun,Liu, Zhijie,Gao, Jinliang,Ma, Miling,Liu, Aihong,Dang, Zhisheng,Liu, Junlong,Ren, Qiaoyun,Jin, Yurong,Yin, Hong,Guan, Guiquan,Chauvin, Alain,Moreau, Emmanuelle,Inoue, Noboru,Thekisoe, Oriel M. M.,Sugimoto, Chihiro.

[13]An attenuated live vaccine based on highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) protects piglets against HP-PRRS. Tong, Guang-Zhi,Tian, Zhi-Jun,An, Tong-Qing,Zhou, Yan-Jun,Peng, Jin-Mei,Hu, Shou-Ping,Wei, Tian-Chao,Jiang, Yi-Feng,Xiao, Yan,Tong, Guang-Zhi.

[14]Effect of Candida albicans on Intestinal Ischemia-reperfusion Injury in Rats. Yan, Lei,Wu, Chun-Rong,Yang, Chun-Hui,Tang, Jian-Guo,Wang, Chen,Tong, Guang-Zhi. 2016

[15]Cetyltriethylammonium bromide stimulating transcription of Bombyx mori nucleopolyhedrovirus gp64 gene promoter mediated by viral factors. Zhou, YJ,Yi, YZ,Zhang, ZF,He, JL,Zhang, YX.

[16]Integrin v1 Modulation Affects Subtype B Avian Metapneumovirus Fusion Protein-mediated Cell-Cell Fusion and Virus Infection. Yun, Bing-Ling,Guan, Xiao-Lu,Liu, Yong-Zhen,Zhang, Yao,Wang, Yong-Qiang,Qi, Xiao-Le,Cui, Hong-Yu,Liu, Chang-Jun,Zhang, Yan-Ping,Gao, Hong-Lei,Gao, Li,Li, Kai,Gao, Yu-Long,Wang, Xiao-Mei,Wang, Xiao-Mei.

[17]Steinernema glaseri surface enolase: Molecular cloning, biological characterization, and role in host immune suppression. Liu, Hua,Zeng, Hongmei,Yao, Qing,Yuan, Jingjing,Zhang, Yuliang,Qiu, Dewen,Yang, Xiufen,Yang, Huaiwen,Liu, Zheng.

[18]Impact of Helicoverpa armigera nucleopolyhedroviruses expressing a cathepsin L-like protease on target and nontarget insect species on cotton. Sun, Xiulian,Wu, Dong,Sun, Xincheng,Jin, Liang,Peng, Huiyin,Hu, Zhihong,Sun, Xiulian,Wu, Dong,Sun, Xincheng,Jin, Liang,Peng, Huiyin,Hu, Zhihong,Ma, Yan,Bonning, Bryony C..

[19]Characterization of the H5N1 Highly Pathogenic Avian Influenza Virus Derived from Wild Pikas in China. Zhou, Jiyong,Sun, Wenbo,Wang, Junhua,Guo, Junqing,Yin, Wei,Yan, Yan,Jiang, Xuetao,Zhou, Jiyong,Sun, Wenbo,Wang, Junhua,Guo, Junqing,Yin, Wei,Wu, Nanping,Li, Lanjuan,Yan, Yan,Jiang, Xuetao,Zhou, Jiyong,Sun, Wenbo,Wang, Junhua,Guo, Junqing,Yan, Yan,Chen, Hualan,Liao, Ming,Luo, Kaijian,Huang, Yu.

[20]Effects of cetyltriethylammonium bromide on the replication of Bombyx mori nucleopolyhedrovirus. Zhou, YJ,Zhang, ZF,He, JL,Zhang, YX.

作者其他论文 更多>>