数字农科院2.0

A SARS-CoV-2 Nanobody Displayed on the Surface of Human Ferritin with High Neutralization Activity

文献类型： 外文期刊

作者： Wenrong Zhang;Haining Wang;Tong Wu;Xintao Gao;Yuting Shang;Zhifang Zhang;Xingjian Liu;Yinü Li

作者机构：

关键词： baculovirus expression system;COVID-19;fenobody;ferritin;silkworm

期刊名称： International Journal of Nanomedicine

ISSN： 1176-9114

年卷期： 2024 年 19 卷

页码：

收录情况： SCIE（2024版）

摘要： Purpose: COVID-19 is rampant throughout the world, which has caused great damage to human lives and seriously hindered the development of the global economy. Aiming at the treatment of SARS-CoV-2, in this study, we proposed a novel fenobody strategy based on ferritin (Fe) self-assembly technology. Methods: The neutralizing nanobody H11-D4 of SARS-CoV-2 fused to the C-terminus of end-modified human ferritin was expressed in E. coli and silkworm baculovirus expression systems. A large number of nanoparticles were successfully self-assembled in silkworms, while relatively few nanoparticles can be observed in the treated products from E. coli by electron microscopy. Subsequently, the fenobody’s expression level and neutralizing activity were then evaluated. Results: The results showed that the IC50 of H11-D4 and fenobody Fe-H11-D4 expressed in E. coli were 171.1 nmol L−1 and 20.87 nmol L−1, respectively. However, the IC50 of Fe-HD11-D4 expressed in silkworms was 1.46 nmol L−1 showing better neutralization activity. Conclusion: Therefore, fenobodies can be well self-assembled in silkworm baculovirus expression system, and ferritin self-assembly technology can effectively improve nanobody neutralization activity.

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