数字农科院2.0

Genome characteristics and the ODV proteome of a second distinct alphabaculovirus from Spodoptera litura

文献类型： 外文期刊

作者： Weisong Gao;Xingjian Liu;Xintao Gao;Tong Wu;Shuang Wei;Zhifang Zhang;Huan Zhang;Yinü Li

作者机构：

关键词： Baculovirus;Genome sequence;ODV proteome;Spodoptera litura Nucleopolyhedrovirus;Virus species demarcation criteria

期刊名称： BMC Genomics

ISSN： 1471-2164

年卷期： 2024 年 25 卷 1 期

页码：

收录情况： SCIE（2024版）

摘要： Background: Spodoptera litura is a harmful pest that feeds on more than 80 species of plants, and can be infected and killed by Spodoptera litura nucleopolyhedrovirus (SpltNPV). SpltNPV-C3 is a type C SpltNPV clone, that was observed and collected in Japan. Compared with type A or type B SpltNPVs, SpltNPV-C3 can cause the rapid mortality of S. litura larvae. Methods: In this study, occlusion bodies (OBs) and occlusion-derived viruses (ODVs) of SpltNPV-C3 were purified, and OBs were observed by scanning electron microscopy (SEM). ODVs were observed under a transmission electron microscope (TEM). Results: Both OBs and ODVs exhibit morphological characteristics typical of nucleopolyhedroviruses (NPVs).The genome of SpltNPV-C3 was sequenced and analyzed; the total length was 148,634 bp (GenBank accession 780,426,which was submitted as SpltNPV-II), with a G + C content of 45%. A total of 149 predicted ORFs were found. A phylogenetic tree of 90 baculoviruses was constructed based on core baculovirus genes. LC‒MS/MS was used to analyze the proteins of SpltNPV-C3; 34 proteins were found in the purified ODVs, 15 of which were core proteins. The structure of the complexes formed by per os infectivity factors 1, 2, 3 and 4 (PIF-1, PIF-2, PIF-3 and PIF-4) was predicted with the help of the AlphaFold multimer tool and predicted conserved sequences in PIF-3. SpltNPV-C3 is a valuable species because of its virulence, and the analysis of its genome and proteins in this research will be beneficial for pest control efforts.

分类号：

  • 相关文献

[1]A Renewed Appreciation of Helicoverpa armigera Nucleopolyhedrovirus BJ (Formerly Helicoverpa assulta Nucleopolyhedrovirus) with Whole Genome Sequencing. Zhao, Lulu,Liu, Xingjian,Tang, Kai,Zhang, Zhifang,Zhang, Huan,Li, Yinu. 2022

[2]The ecdysteroid UDP-glucosyltransferase gene promoter from Autographa californica multicapsid nucleopolyhedrovirus. Shen, XJ,Yi, YZ,Tang, SM,Zhang, ZF,Li, YR,He, JL.

[3]Complete Genome Sequence of Peanut stripe virus Isolated in China. Xu, Manlin,Gao, Fangluan,Yang, Jinguang,Wu, Juxiang,Xie, Lianhui,Chi, Yucheng. 2014

[4]Genome-Wide Investigation of WRKY Transcription Factors Involved in Terminal Drought Stress Response in Common Bean. Wu, Jing,Wang, Lanfen,Wang, Shumin,Chen, Jibao. 2016

[5]IDENTIFICATION OF GENOME RECOMBINATION AMONG APPLE STEM PITTING VIRUS ISOLATES. Li, Z.,Liu, P.,Sun, L.,Wu, Y.,Li, Z.,Liu, P.,Sun, L.,Wu, Y.,Li, Z.,Kondo, H.,Andika, I. B..

[6]Comparative genomic analysis between newly sequenced Brucella suis Vaccine Strain S2 and the Virulent Brucella suis Strain 1330. Di, Dong-dong,Tian, Li-li,Kang, Jing-li,Ni, Bo,Fan, Wei-xing,Mao, Xiang,Jiang, Hai,Zhang, Wen,Cui, Bu-yun,Yi, Xin-ping,Ye, Feng,Zhong, Qi,He, You-yu,Xia, Lin,Yu, Yao. 2016

[7]Complete genome sequence of Bacillus subtilis SG6 antagonistic against Fusarium graminearum. Zhao, Yueju,Sangare, Lancine,Wang, Yao,Folly, Yawa Minnie Elodie,Selvaraj, Jonathan Nimal,Xing, Fuguo,Zhou, Lu,Wang, Yan,Liu, Yang,Zhao, Yueju,Sangare, Lancine,Wang, Yao,Folly, Yawa Minnie Elodie,Selvaraj, Jonathan Nimal,Xing, Fuguo,Zhou, Lu,Wang, Yan,Liu, Yang.

[8]Development of mapped simple sequence repeat markers from common bean (Phaseolus vulgaris L.) based on genome sequences of a Chinese landrace and diversity evaluation. Chen, Mingli,Wu, Jing,Wang, Lanfeng,Jia, Jizeng,Wang, Shumin,Zhang, Xiaoyan,Blair, Matthew W..

[9]Complete genome sequence of Pseudomonas azotoformans S4, a potential biocontrol bacterium. Fang, Yang,Wu, Lijuan,Chen, Guoqing,Feng, Guozhong,Fang, Yang.

[10]Complete genome sequence of Streptomyces griseochromogenes ATCC 14511(T), a producer of nucleoside compounds and diverse secondary metabolites. Wu Lijuan,Chen, Guoqing,Feng, Guozhong.

[11]The complete genome sequence of Citrus vein enation virus from China. Huang Ai-jun,Song Zhen,Cao Meng-ji,Chen Hong-ming,Li Zhong-an,Zhou Chang-yong,Huang Ai-jun. 2015

[12]Genome-wide identification and analysis of the MADS-box gene family in sesame. Wei, Xin,Wang, Linhai,Yu, Jingyin,Zhang, Yanxin,Li, Donghua,Zhang, Xiurong.

[13]Complete genome sequence of Streptomyces cyaneogriseus ssp noncyanogenus, the thermotolerant producer of commercial antibiotics nemadectin. Li, Chuang,Zhang, Bo,Jin, Pinjiao,Wang, Jijia,Zhang, Ji,Wang, Xiangjing,Xiang, Wensheng,He, Hairong,Xiang, Wensheng.

[14]Bolbase: a comprehensive genomics database for Brassica oleracea. Yu, Jingyin,Zhao, Meixia,Tong, Chaobo,Huang, Shunmou,Tehrim, Sadia,Hua, Wei,Liu, Shengyi,Wang, Xiaowu,Liu, Yumei,Zhao, Meixia. 2013

[15]Fine genetic mapping localizes cucumber scab resistance gene Ccu into an R gene cluster. Kang, Houxiang,Yang, Yuhong,Zhang, Zhonghua,Zhang, Shengping,Mao, Zhenchuan,Cheng, Guohua,Gu, Xingfang,Huang, Sanwen,Xie, Bingyan,Weng, Yiqun,Kang, Houxiang,Yang, Yuhong,Zhang, Zhonghua,Zhang, Shengping,Mao, Zhenchuan,Cheng, Guohua,Gu, Xingfang,Huang, Sanwen,Xie, Bingyan.

[16]Complete genome sequence of an aromatic compound degrader Arthrobacter sp YC-RL1. Ren, Lei,Jia, Yang,Ruth, Nahurira,Zhao, Baisuo,Yan, Yanchun.

[17]Isolation and sequence analysis of a canine distemper virus from a raccoon dog in Jilin Province, China. Cheng, Yuening,Wang, Jianke,Zhang, Miao,Zhao, Jianjun,Shao, Xiqun,Zhao, Hang,Lin, Peng,Wu, Hua,Ma, Zengjun.

[18]Complete genome sequence of Bacillus amyloliquefaciens L-S60, a plant growth-promoting and antifungal bacterium. Qin, Yuxuan,Han, Yuzhu,Zhang, Bao,Li, Pinglan,Yu, Yaqiong,Shang, Qingmao.

[19]Complete nucleotide sequences of two isolates of Cherry virus A from sweet cherry in China. Gao Rui, Li Shifang, Lu Meiguang. 2016

[20]Complete Genome Sequence Of Gordonia S.p Yc-Jh1, A Bacterium E fficiently Degrading A Wide Range Of Phthalic Acid Esters. Fan, SH, Wang, JH, Li, K, Yang, T, Jia, Y, Zhao, BS, Yan, YC. 2018

作者其他论文 更多>>