数字农科院2.0

Recent advances in CRISPR/Cas-based electrochemical biosensors for pathogen detection

文献类型： 外文期刊

作者： Cui, Jiuying;Cheng, Xueqing;Guo, Xinyan;Li, Dandan;Xu, Ziqi;Qian, Wanqiang;Wang, Zhaoyin;Dai, Zhihui

作者机构：

关键词： CRISPR/Cas;Pathogen analysis;Electrochemical biosensor;Target recognition

期刊名称： TRAC-TRENDS IN ANALYTICAL CHEMISTRY

ISSN： 0165-9936

年卷期： 2025 年 196 卷

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： Pathogenic microorganisms, including bacteria and viruses, remain major causes of infectious diseases and foodrelated illnesses. Traditional methods for pathogen detection are often constrained by lengthy processing times, cross-reactivity, and operational complexity. Electrochemical biosensors have emerged as a transformative approach, offering rapid, cost-effective, and highly accurate identification capabilities. In parallel, CRISPR/Cas systems, characterized by programmable nucleic acid recognition and nuclease activity, have demonstrated remarkable potential for pathogen detection, particularly when integrated with electrochemical biosensors. This review begins by classifying the biological targets of pathogens alongside their corresponding amplification strategies, and then examines the critical roles of CRISPR family members in target recognition and signal transduction. Furthermore, recent progress in CRISPR/Cas-based electrochemical platforms is highlighted, emphasizing improvements in sensitivity, specificity, and efficiency, aiming to inspire next-generation biosensors for advanced pathogen diagnostics.

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