数字农科院2.0

Screening of resistance to cucumber green mottle mosaic virus in bottle gourd mutated by Ethyl Methane Sulphonate (EMS)

文献类型： 外文期刊

作者： AL-Kubati, Asma Mohammed Saeed;Kang, Baoshan;Abbas, Aqleem;Kaseb, Mohamed Omar;Gu, Qinsheng

作者机构：

关键词： Bottle gourd;Cucumber green mottle mosaic virus;Ethyl methane sulphonate;M3 lines Resistance

期刊名称： AUSTRALASIAN PLANT PATHOLOGY

ISSN： 0815-3191

年卷期： 2022 年

页码：

收录情况： SCIE（2022版）

摘要： Cucumber green mottle mosaic virus (CGMMV) is a major threat to cucurbits-producing regions worldwide. Conventional breeding process takes much time to cross plants and to select a good variety. In contrast to conventional breeding, mutation breeding accelerates the process of cultivar development and increases variety within a crop species. Hence, the aim of this study was to develop bottle gourd lines that were resistant to CGMMV using Ethyl Methane Sulfonate (EMS) mutagenesis. By treating wild-type seeds (M0) with EMS, bottle gourd lines (M1) were produced. M1 lines were self-pollinated; as a result, 303 M2 families were obtained. From M2 families, a total of 2444 M3 lines were obtained to screen resistance to CGMMV. The M3 lines were then classified into 5 levels based on the development of symptoms 11-30 days post-inoculation, with very light symptoms 13.91%, light symptoms 34.0%, mild symptoms 33.71%, severe symptoms 4.62%, and death lines 4.62%, respectively. About 278 (11.38%) M3 lines in glasshouse experiments showed no symptoms at 30 dpi. Using a double-antibody sandwich enzyme-linked immunosorbent assay at 60 dpi, 44 M3 lines out of 278 were determined to be asymptomatic and negative for CGMMV. These lines were then transplanted in the field. Four of the 44 M3 lines were asymptomatic in the field and tested negative for CGMMV using the DAS-ELISA. The new findings show that using EMS-induced mutations to create novel CGMMV highly resistant bottle gourd cultivars could be a viable option.

分类号：

  • 相关文献

[1]Transmission Efficiency of Cucumber green mottle mosaic virus via Seeds, Soil, Pruning and Irrigation Water. Li, Jun-Xiang,Liu, Shan-Shan,Gu, Qin-Sheng.

[2]Completion sequence and cloning of the infectious cDNA of a chb isolate of cucumber green mottle mosaic virus. Zhao, X.,Wang, Y.,Cao, K.,Liu, Y..

[3]Molecular Characterization and Distribution of Cucumber green mottle mosaic virus in China. Wang, Xifeng.

[4]Real time TaqMan RT-PCR assay for the detection of Cucumber green mottle mosaic. Chen Hongyun,Chen Qing,Lin Shiming,Chen Hongyun,Zhao Wenjun,Zhu Shuifang,Gu Qinsheng. 2008

[5]Mapping Subgenomic Promoter Of Coat Protein Gene Of Cucumber Green Mottle Mosaic Virus. Liu, M, Liu, LM, Wu, HJ, Kang, BS, Gu, QS. 2020

[6]A Cucumber Green Mottle Mosaic Virus Vector For Virus-Induced Gene Silencing In Cucurbit Plants. Liu, M, Liang, ZL, Aranda, MA, Hong, N, Liu, LM, Kang, BS, Gu, QS. 2020

[7]Interaction between cucumber green mottle mosaic virus MP and CP promotes virus systemic infection. Shi, Ya-Juan,Yang, Xue,Yang, Ling-Ling,Li, Qing-Lun,Liu, Xiao-Min,Han, Xiao-Yu,Gu, Qin-Sheng,Li, Hong-Lian,Chen, Lin-Lin,Liu, Yiqing,Shi, Yan. 2023

[8]The 96Th Amino Acid Of T.he Coat Protein Of C ucumber Green Mottle Mosaic Virus Affects Virus Infectivity. Zhang, ZW, Liu, LM, Wu, HJ, Liu, LF, Kang, BS, Peng, B, Gu, QS. 2018

[9]Argonaute 1 and 5 proteins play crucial roles in the defence against cucumber green mottle mosaic virus in watermelon. Liu, Mei,Wu, Huijie,Hong, Ni,Kang, Baoshan,Peng, Bin,Liu, Liming,Gu, Qinsheng. 2023

[10]Cucumber green mottle mosaic virus encodes additional small proteins with specific subcellular localizations and virulence function. Gong, Pan,Gao, Mengxin,Chen, Yalin,Zhang, Mingzhen,Huang, Yucong,Hu, Xiaohua,Zhao, Siwen,Zhang, Hui,Pan, Mengjiao,Cao, Buwei,Shen, Qingtang,Liu, Yong,Lozano-Duran, Rosa,Wang, Aiming,Zhou, Xueping,Li, Fangfang. 2025

[11]Exogenous Application of IR-Specific dsRNA Inhibits Infection of Cucumber Green Mottle Mosaic Virus in Watermelon. Wang, Yanhui,Liu, Liming,Fan, Yongqiang,Han, Yanli,Liang, Zhiling,Geng, Yanfei,Liu, Fengnan,Gu, Qinsheng,Kang, Baoshan,Luo, Chaoxi. 2025

作者其他论文 更多>>