数字农科院2.0

The whole genomic analysis of the Orf virus strains ORFV-SC and ORFV-SC1 from the Sichuan province and their weak pathological response in rabbits

文献类型： 外文期刊

作者： Guoyu Du;Jinyan Wu;Cheng Zhang;Xiaoan Cao;Lingxia Li;Jijun He;Yong Zhang;Youjun Shang

作者机构：

关键词： Genome sequencing;Orf virus;ORFV-attenuated vaccines;Phylogenetic tree;Secondary and tertiary structure

期刊名称： Functional & integrative genomics

ISSN： 1438-7948

年卷期： 2023 年 23 卷 2 期

页码：

收录情况： SCIE（2023版）

摘要： The Orf virus (ORFV) is a member of the Parapoxvirus genus of the Poxviridae family and can cause contagious diseases in sheep, goats, and wild ungulates. In the present study, two ORFV isolates (ORFV-SC isolated from Sichuan province and ORFV-SC1 produced by 60 passages of ORFV-SC in cells) were sequenced and compared to multiple ORFVs. The two ORFV sequences had entire genome sizes of 14,0707 bp and 141,154 bp, respectively, containing 130 and 131 genes, with a G + C content of 63% for the ORFV-SC sequence and 63.9% for the ORFV-SC1 sequence. Alignment of ORFV-SC and ORFV-SC1 with five other ORFV isolates revealed that ORFV-SC, ORFV-SC1, and NA1/11 shared > 95% nucleotide identity with 109 genes. Five genes (ORF007, ORF20, ORF080, ORF112, ORF116) have low amino acids identity between ORFV-SC and ORFV-SC1. Mutations in amino acids result in changes in the secondary and tertiary structure of ORF007, ORF020, and ORF112 proteins. The phylogenetic tree based on the complete genome sequence and 37 single genes revealed that the two ORFV isolates originated from sheep. Finally, animal experiments demonstrated that ORFV-SC1 is less harmful to rabbits than ORFV-SC. The exploration of two full-length viral genome sequences provides valuable information in ORFV biology and epidemiology research. Furthermore, ORFV-SC1 demonstrated an acceptable safety profile following animal vaccination, indicating its potential as a live ORFV vaccine.

分类号：

  • 相关文献

[1]Effect of ORF119 gene deletion on the replication and virulence of orf virus. Qiao, J.,Yang, H. B.,Peng, Y. L.,Meng, Q. L.,Chen, C.,Ma, Y.,Xie, K.,Liu, T. L.,Chen, C. F.,Cai, X. P..

[2]Development of a fluorescent probe-based recombinase polymerase amplification assay for rapid detection of Orf virus. Yang, Yang,Qin, Xiaodong,Wang, Guangxiang,Zhang, Yuen,Shang, Youjun,Zhang, Zhidong,Zhang, Yuen. 2015

[3]Detection of genetic variations in Orf virus isolates epidemic in Xinjiang China. Yang, Haibo,Meng, Qingling,Qiao, Jun,Peng, Yelong,Xie, Kun,Liu, Yucheng,Zhao, Hailong,Chen, Chuangfu,Cai, Xuepeng.

[4]Identification and function analysis of the host cell protein that interacted with Orf virus Bcl-2-like protein ORFV125. Tian, Hong,Chen, Yan,Wu, Jinyan,Lin, Tong,Liu, Xiangtao.

[5]Comparison of a loop-mediated isothermal amplification for orf virus with quantitative real-time PCR. Wang, Guangxiang,Wang, Yanhua,Liu, Xiangtao,Tian, Hong,Shang, Youjun,Wang, Guangxiang,Shang, Youjun,Wang, Yanhua,Liu, Xiangtao. 2013

[6]Human Infection with Orf Virus from Goats in China, 2012. Zhang, Keshan,Liu, Yongjie,Kong, Hanjin,Shang, Youjun,Liu, Xiangtao. 2014

[7]Parapoxvirus orf virus infection induces an increase in interleukin-8, tumour necrosis factor-alpha, and decorin in goat skin fibroblast cells. Wang, Lingling,Lu, Bingzhou,Zheng, Haixue,Zhang, Keshan,Liu, Xiangtao.

[8]Molecular detection of a mixed infection of Goatpox virus, Orf virus, and Mycoplasma capricolum subsp capripneumoniae in goats. Chu, Yuefeng,Yan, Xinmin,Gao, Pengcheng,Zhao, Ping,He, Ying,Liu, Jixing,Lu, Zhongxin. 2011

[9]Development of an isothermoal amplification-based assay for rapid visual detection of an Orf virus. Yang, Yang,Qin, Xiaodong,Wang, Guangxiang,Jin, Jiaxin,Shang, Youjun,Zhang, Zhidong,Jin, Jiaxin. 2016

[10]Fast Construction Of A Reference G.enome: Challenges And Opportunities U sing 'Royal Gala' Apple As A Case Study. Cong, P.,Chagne, D.,Deng, C. H.,Zhang, C.,Bus, V.,Yao, J. -L.,Sullivan, S.,Tian, Y.,Liachko, I.,Gardiner, S. E.,Gleave, A.. 2018

[11]Recent Insights Into Cotton Functional G.enomics: Progress And Future P erspectives. Malik, Waqas,Song, Guoli,Wang, Qiaolian,Yang, Qiuhong,Zuo, Dongyun,Zhang, Youping,Cheng, Hailiang,Abid, Muhammad Ali,Ashraf, Javaria. 2018

[12]Rhizobium oryziradicis sp nov., isolated from rice roots. Zhao, Juan-Juan,Zhang, Jun,Zhang, Rui-Jie,Zhang, Cai-Wen,Zhang, Xiao-Xia,Sun, Lei,Yin, Hua-Qun.

[13]Isolation and pathogenicity of the mammalian orthoreovirus MPC/04 from masked civet cats. Li, Zhijie,Shao, Yuhao,Liu, Chunguo,Liu, Dafei,Guo, Dongchun,Qiu, Zheng,Tian, Jin,Zhang, Xiaozhan,Liu, Shengwang,Qu, Liandong. 2015

[14]Nitrogen fixation island and rhizosphere competence traits in the genome of root-associated Pseudomonas stutzeri A1501. Elmerich, Claudine,Yan, Yongliang,Dou, Yuetan,Chen, Ming,Ping, Shuzhen,Lu, Wei,Zhang, Wei,Yao, Ziying,Li, Hongquan,Liu, Wei,He, Sheng,Geng, Lizhao,Yu, Haiying,Zhan, Yuhua,Li, Danhua,Lin, Min,Yan, Yongliang,Yang, Jian,Peng, Junping,Zhang, Xiaobing,Yang, Fan,Jin, Qi,Dou, Yuetan,Yu, Haiying,Zhan, Yuhua,Li, Danhua,Ping, Shuzhen,He, Sheng,Lin, Min,Lin, Zhanglin,Wang, Yiping.

[15]Characterization and pathogenicity of a novel mammalian orthoreovirus from wild short-nosed fruit bats. Li, Zhijie,Liu, Dafei,Ran, Xuhua,Liu, Chunguo,Guo, Dongchun,Hu, Xiaoliang,Tian, Jin,Zhang, Xiaozhan,Shao, Yuhao,Liu, Shengwang,Qu, Liandong.

[16]Transposon insertions regulate genome-wide allele-specific expression and underpin flower colour variations in apple (Malus spp.). Tian, Yi,Thrimawithana, Amali,Ding, Tiyu,Guo, Jian,Gleave, Andrew,Chagne, David,Ampomah-Dwamena, Charles,Ireland, Hilary S.,Schaffer, Robert J.,Luo, Zhiwei,Wang, Meili,An, Xiuhong,Wang, Dajiang,Gao, Yuan,Wang, Kun,Zhang, Hengtao,Zhang, Ruiping,Zhou, Zhe,Yan, Zhenli,Zhang, Liyi,Zhang, Caixia,Cong, Peihua,Deng, Cecilia H.,Yao, Jia-Long. 2022

[17]Insights Into Xylan Degradation And H.aloalkaline Adaptation Through Whole-Genome A nalysis Of Alkalitalea Saponilacus, An Anaerobic Haloalkaliphilic Bacterium Capable Of Secreting Novel Halostable Xylanase. Liao, ZY, Holtzapple, M, Yan, YC, SWang, HS, Li, J, Zhao, BS. 2019

[18]Genome sequence of the asian honeybee in pakistan sheds light on its phylogenetic relationship with other honeybees. Hongwei Tan,Muhammad Naeem,Hussain Ali,Muhammad Shakeel,Haiou Kuang,Ze Zhang,Cheng Sun. 2021

[19]Recent insights into cotton functional genomics: progress and future perspectives. 宋国立. 2018

[20]Arthrobacter wenxiniae sp. nov., a novel plant growth-promoting rhizobacteria species harbouring a carotenoids biosynthetic gene cluster. Sun, Yu-chen,Sun, Pengbo,Xue, Jing,Du, Yunpeng,Yan, Hui,Wang, Li-wei,Yi, Xin-xin,Sun, Jian-guang,Zhang, Xiuhai,Gao, Jun-lian. 2021

作者其他论文 更多>>