数字农科院2.0

Engineering His-Tagged Senecavirus A for One-Step Purification of Viral Antigens

文献类型： 外文期刊

作者： Junhao Fan;Peiyu Xiao;Dongni Kong;Xinran Liu;Liang Meng;Tongqing An;Xuehui Cai;Haiwei Wang;Li Yu

作者机构：

关键词： Antigen purification;His-tagged virus;Senecavirus A

期刊名称： Vaccines

ISSN： 2076-393X

年卷期： 2022 年 10 卷 2 期

页码：

收录情况： JCR（2021版）

摘要： Senecavirus A (SVA) is a picornavirus that causes vesicular disease in swine, and the in-activated vaccine is used to prevent and control SVA infection. To develop a new chromatography strategy for the purification and concentration of SVA vaccine antigens, we inserted a 6×His-tag at the VP1 C-terminal of the SVA/HLJ/CHA/2016 in an infectious clone to rescue a His-tagged SVA. The constructed and rescued recombinant virus, named as rSVA-His, exhibited similar growth ki-netics to that of its parental virus. In addition, the expression of a 6×His-tag on the surface of SVA showed genetic stability in cell passages in vitro, which allowed one-step purification of SVA antigens by Ni2+ affinity columns. Furthermore, the immunogenicity of the inactivated rSVA-His was evaluated by inoculating rabbits and detecting neutralizing antibodies. The animals receiving two doses of the inactivated rSVA-His emulsified with oil adjuvant developed a high titer of neutralizing antibodies, indicating that SVA VP1 is tolerant to His-tag insertion without detriment to its antigenicity. In summary, the constructed 6×His-tagged SVA may offer a feasible approach to the affinity purification and concentration of antigens in the process of SVA inactivated vaccine pro-duction.

分类号：

  • 相关文献

[1]Emergence and whole-genome sequence of Senecavirus A in Colombia. Sun, D.,Sun, D.,Vannucci, F.,Knutson, T. P.,Marthaler, D. G.,Corzo, C..

[2]Identification of B-cell epitopes on structural proteins VP1 and VP2 of Senecavirus A and development of a multi-epitope recombinant protein vaccine. Zhongwang Zhang,Fei Yao,Jianliang Lv,Yaozhong Ding,Xinsheng Liu,Liping Zhang,Zhongyuan Ma,Peng Zhou,Yonglu Wang,Huichen Guo,Li Pan. 2023

[3]A novel rapid detection of Senecavirus A using recombinase polymerase amplification (RPA) coupled with lateral flow (LF) dipstrip. Huibao Wang,Jinjie Dong,Tao Zhang,Fan Wang,Rui Yang,Yong Zhang,Xingxu Zhao. 2022

[4]Evaluation of antibody response in sows after vaccination with senecavirus a vaccine and the effect of maternal antibody transfer on antibody dynamics in offspring. Fan Yang,Zixiang Zhu,Huanan Liu,Weijun Cao,Wei Zhang,Ting Wei,Min Zheng,Keshan Zhang,Hong Tian,Qiaoying Zeng,Xuepeng Cai,Haixue Zheng. 2021

[5]Senecavirus A 2B protein suppresses type I interferon production by inducing the degradation of MAVS. Huanan Liu,Kangli Li,Wenzhe Chen,Fan Yang,Weijun Cao,Keshan Zhang,Pengfei Li,Lijie Tang,Zixiang Zhu,Haixue Zheng. 2022

[6]Pathogenicity Analysis of Weaned Piglets Challenged With Novel Emerging Senecavirus A in Fujian, China. Cun Liu,Yanhan Liu,Xiubo Li,Lin Liang,Shangjin Cui. 2021

[7]IFIT3 mediated the type I interferon antiviral response by targeting Senecavirus A entry, assembly and release pathways. Fan Xu,Wen Dang,Tao Li,Yannan Wang,Fan Yang,Haixue Zheng. 2022

[8]Modeling senecavirus a replication in immortalized porcine alveolar macrophages triggers a robust interferon-mediated immune response that conversely constrains viral replication. Wen Dang,Tao Li,Fan Xu,Yannan Wang,Fan Yang,Haixue Zheng. 2023

[9]Development and Evaluation of a Competitive Enzyme-Linked Immunosorbent Assay Based on Swine Monoclonal Antibodies for Detecting Neutralizing Antibodies against Senecavirus A. Ma, Xueqing,Huang, Jiaxin,Li, Kun,Ding, Kailu,Fu, Yuanfang,Zhang, Jing,Zhao, Zhixun,Li, Pinghua,Bai, Xingwen,Li, Dong,Liu, Xia,Zeng, Qiaoying,Liu, Zaixin,Sun, Pu,Lu, Zengjun. 2023

[10]Identification of Potential Novel B-Cell Epitopes of Capsid Protein VP2 in Senecavirus A. Ru, Yi,Hao, Rongzeng,Wu, Chunping,Li, Yajun,Lu, Bingzhou,Liu, Huanan,Tian, Hong,Li, Dan,Shi, Zhengwang,Luo, Juncong,Ma, Kun,Zhang, Guicai,Liu, Xiangtao,Zheng, Haixue. 2023

[11]Emergence And Complete Genome Of S.enecavirus A In Pigs O f Henan Province In China, 2017. Wang, HB, Tian, B, Lv, HL, Wang, F, Zhang, T, Wang, CY, Zhang, YD, Dong, JJ. 2019

[12]Global Phosphoproteomics Analysis of IBRS-2 Cells Infected With Senecavirus A. Jieyi Li,Zhongwang Zhang,Jianliang Lv,Zhongyuan Ma,Li Pan,Yongguang Zhang. 2022

[13]Development Of A Novel Sybr Green I-Based Quantitative Rt-Pcr Assay For Senecavirus A Detection In Clinical Samples Of Pigs. Mu, SY, Abdullah, SW, Zhang, Y, Han, SC, Guo, HC, Li, M, Dong, H, Xu, J, Teng, ZD, Wen, XB, Sun, SQ. 2020

[14]Senecavirus A as an Oncolytic Virus: Prospects, Challenges and Development Directions. Dankun Luo,Haiwei Wang,Qiang Wang,Wenping Liang,Bo Liu,Dongbo Xue,Yang Yang,Biao Ma. 2022

[15]Development of an indirect ELISA using a novel linear epitope at the C-terminal region of the VP2 protein to specifically detect antibodies against Senecavirus A. Zhongyuan Ma,Jianliang Lv,Zhongwang Zhang,Li Pan. 2022

[16]Impaired interferon response in senecavirus A infection and identification of 3Cpro as an antagonist. Zhang, Xiangle,Li, Pengfei,Chen, Wenzhe,Zhang, Shilei,Li, Kangli,Ru, Yi,Zhao, Zhenxiang,Cao, Weijun,Yang, Fan,Tian, Hong,Guo, Jianhong,He, Jijun,Zhu, Zixiang,Zheng, Haixue. 2024

[17]Development and optimization of a double antigen sandwich ELISA detecting for Senecavirus A antibodies based on VP2 protein. Chen, Jie,Shi, Zhengwang,Luo, Juncong,Jia, Caixia,Zhang, Xiaoyang,Wei, Juanjuan,Li, Shuaipeng,Zhu, Yuqian,Xi, Tao,Zhou, Jing,He, Yindi,Shi, Xintai,Liao, Huanchen,Tian, Hong,Zheng, Haixue. 2024

[18]A neutralizing nanobody-based liquid-phase blocking ELISA to assess the protective potency of Senecavirus A vaccine. Mu, Suyu,Pan, Songjia,Dong, Hu,Wu, Jinen,Zhang, Yun,Yin, Shuanghui,Wang, Jianing,Wei, Tian,Wen, Xiaobo,Guo, Huichen,Sun, Shiqi. 2025

[19]VP3 protein of Senecavirus A promotes viral IRES-driven translation and attenuates innate immunity by specifically relocalizing hnRNPA2B1. Li, Lu,Li, Xinwei,Zhong, Han,Li, Mingyang,Wan, Bo,He, Wenrui,Zhang, Yuhang,Du, Yongkun,Chen, Dongjie,Zhang, Wei,Ji, Pengchao,Jiang, Dawei,Han, Shichong. 2024

[20]Epitope Mapping of Senecavirus A 3A Protein Using Monoclonal Antibodies. Meng, Liang,Tian, Xiao-Xiao,Xiang, Xu-Yan,Qi, Xin-Yu,Zhou, Han-Rong,Xiao, Pei-Yu,An, Tong-Qing,Meng, Fan-Dan,Wang, Hai-Wei. 2025

作者其他论文 更多>>