数字农科院2.0

In-locus gene silencing in plants using genome editing

文献类型： 外文期刊

作者： Shen, Rundong;Yao, Qi;Tan, Xinhang;Ren, Wendan;Zhong, Dating;Zhang, Xuening;Li, Xinbo;Dong, Chao;Cao, Xuesong;Tian, Yifu;Zhu, Jian-Kang;Lu, Yuming

作者机构：

关键词： downregulation;genome editing;molecular breeding;rice;sequence insertion

期刊名称： NEW PHYTOLOGIST

ISSN： 0028-646X

年卷期： 2024 年

页码：

收录情况： SCIE（2024版）

摘要： Gene silencing is crucial in crop breeding for desired trait development. RNA interference (RNAi) has been used widely but is limited by ectopic expression of transgenes and genetic instability. Introducing an upstream start codon (uATG) into the 5 ' untranslated region (5 ' UTR) of a target gene may 'silence' the target gene by inhibiting protein translation from the primary start codon (pATG). Here, we report an efficient gene silencing method by introducing a tailor-designed uATG-containing element (ATGE) into the 5 ' UTR of genes in plants, occupying the original start site to act as a new pATG. Using base editing to introduce new uATGs failed to silence two of the tested three rice genes, indicating complex regulatory mechanisms. Precisely inserting an ATGE adjacent to pATG achieved significant target protein downregulation. Through extensive optimization, we demonstrated this strategy substantially and consistently downregulated target protein expression. By designing a bidirectional multifunctional ATGE4, we enabled tunable knockdown from 19% to 89% and observed expected phenotypes. Introducing ATGE into Waxy, which regulates starch synthesis, generated grains with lower amylose, revealing the value for crop breeding. Together, we have developed a programmable and robust method to knock down gene expression in plants, with potential for biological mechanism exploration and crop enhancement.

分类号：

  • 相关文献

[1]Novel Plant Breeding Techniques Shake Hands with Cereals to Increase Production. Muhammad Haroon,Xiukang Wang,Rabail Afzal,Muhammad Mubashar Zafar,Fahad Idrees,Maria Batool,Abdul Saboor Khan,Muhammad Imran. 2022

[2]Molecular breeding of tomato: Advances and challenges. Du, Minmin,Sun, Chuanlong,Deng, Lei,Zhou, Ming,Li, Junming,Du, Yongchen,Ye, Zhibiao,Huang, Sanwen,Li, Tianlai,Yu, Jingquan,Li, Chang-Bao,Li, Chuanyou. 2025

[3]Cotton under heat stress: a comprehensive review of molecular breeding, genomics, and multi-omics strategies. Tahira Luqman,Manzoor Hussain,Syed Riaz Ahmed,Iram Ijaz,Zahra Maryum,Sahar Nadeem,Zafran Khan,Sana Muhy Ud Din Khan,Mohammad Aslam,Yongming Liu,Muhammad Kashif Riaz Khan. 2025

[4]Study on heterosis of inter-subspecies between indica and japonica rice (Oryza sativa L.) using chromosome segment substitution lines. Yu, CY,Wan, JM,Zhai, HQ,Wang, CM,Jiang, L,Xiao, YH,Liu, YQ.

[5]The Ghd7 transcription factor represses ARE1 expression to enhance nitrogen utilization and grain yield in rice. Qing Wang,Qingmei Su,Jinqiang Nian,Jian Zhang,Meng Guo,Guojun Dong,Jiang Hu,Rongsheng Wang,Changshuo Wei,Guanwen Li,Wan Wang,Hui-Shan Guo,Shaoyang Lin,Wenfeng Qian,Xianzhi Xie,Qian Qian,Fan Chen,Jianru Zuo. 2021

[6]Uncovering the breeding contribution of transposable elements from landraces to improved varieties through pan-genome-wide analysis in rice. Li, Xiaoxia,Dai, Xiaofan,He, Huiying,Chen, Wu,Qian, Qian,Shang, Lianguang,Guo, Longbiao,He, Wenchuang. 2025

[7]A Review of Reducing Cadmium Pollution in the Rice-Soil System in China. Guan, Meiyan,Xia, Yuchun,Zhang, Weixing,Chen, Mingxue,Cao, Zhenzhen. 2025

[8]Integrative genomic and transcriptomic approaches decipher pre-harvest sprouting resistance in rice. Yang, Lv,Yang, Renyuan,Wang, Yuxuan,Asad, Muhammad Asad Ullah,Wang, Yueying,Chang, Zhiyuan,Miao, Tianhui,Yang, Shudong,Wei, Yiting,Wu, Shanshan,Bao, Jiaxue,Wu, Mingming,Ye, Jing,Zhai, Rongrong,Ye, Shenghai,Zhang, Xiaoming,Zeng, Faliang,Yu, Faming. 2025

[9]水稻淡褐斑叶突变体lbsll的遗传分析与基因定位. 奉保华,杨杨,施勇烽,林璐,陈洁,黄奇娜,魏彦林,HeiLEUNG,吴建利. 2012

[10]OsBTF3转基因水稻对病原茵侵染的反应和防卫基因的表达分析. 陈华民,吴茂森,何晨阳. 2012

[11]Genome-edited ATP BINDING CASSETTE B1 transporter SD8 knockouts show optimized rice architecture without yield penalty. Ruihong Qu,Pingxian Zhang,Qing Liu,Yifan Wang,Weijun Guo,Zhuoying Du,Xiulan Li,Liwen Yang,Shuangyong Yan,Xiaofeng Gu. 2022

[12]High-throughput genome editing in rice with a virus-based surrogate system. Tian, Yifu,Zhong, Dating,Li, Xinbo,Shen, Rundong,Han, Han,Dai, Yuqin,Yao, Qi,Zhang, Xuening,Deng, Qi,Cao, Xuesong,Zhu, Jian-Kang,Lu, Yuming. 2022

[13]Fine-tuning OsCPK18/OsCPK4 activity via genome editing of phosphorylation motif improves rice yield and immunity. Li, Hong,Zhang, Yun,Wu, Caiyun,Bi, Jinpeng,Chen, Yache,Jiang, Changjin,Cui, Miaomiao,Chen, Yuedan,Hou, Xin,Yuan, Meng,Xiong, Lizhong,Yang, Yinong,Xie, Kabin. 2022

[14]CRISPR-Based Genome Editing: Advancements and Opportunities for Rice Improvement. Zegeye W.A.,Tsegaw M.,Zhang Y.,Cao L.. 2022

[15]CRISPR/Cas systems: The link between functional genes and genetic improvement. Yong Huang,Huirong Dong,Meiqi Shang,Kejian Wang. 2021

[16]CRISPR/Sc++-mediated genome editing in rice. Guigen Ma,Yongjie Kuang,Zhenwan Lu,Xueqi Li,Ziyan Xu,Bin Ren,Xueping Zhou,Huanbin Zhou. 2021

[17]Precise tiller angle control by manipulating TAC1 expression in rice. Yin, Tao,Tai, Yuxin,Sun, Yao,Cheng, Zixiang,Wu, Chuanyin,Sui, Yi. 2025

[18]Transcriptomics Analysis Identified Candidate Genes Colocalized with Seed Dormancy QTLs in Rice (Oryza sativa L.). Qin, Huaide,Xie, Kun,Jiang, Ling,Wan, Jianmin,Wu, Fuqing,Cheng, Zhijun,Guo, Xiuping,Zhang, Xin,Wang, Jie,Lei, Cailin,Wang, Jiulin,Mao, Long,Wan, Jianmin.

[19]The Genetic Architecture of Flowering Time and Photoperiod Sensitivity in Maize as Revealed by QTL Review and Meta Analysis. Xu, Jie,Liu, Jian,Cao, Moju,Wang, Jing,Lan, Hai,Lu, Yanli,Pan, Guangtang,Rong, Tingzhao,Liu, Yaxi,Xu, Yunbi,Xu, Yunbi. 2012

[20]Improvement of drought and salt tolerance in Arabidopsis and Lotus corniculatus by overexpression of a novel DREB transcription factor from Populus euphratica. Zhou, Mei-Liang,Ma, Jiang-Tao,Zhao, Yang-Min,Tang, Yi-Xiong,Wu, Yan-Min,Zhao, Yang-Min,Wei, Ya-Hui.

作者其他论文 更多>>