数字农科院2.0

Multi-mode probe based on dual colorimetric and photothermal later flow immunoassay for the ultrasensitive determination of benzo[a]pyrene in vegetable oils

文献类型： 外文期刊

作者： Yuting Yuan;Xiaoqian Tang;Liangxiao Zhang;Qi Zhang;Fei Ma;Peiwu Li

作者机构：

关键词： Benzo[a]pyrene;Hazardous substances;Lateral flow immunoassay;Multi mode;Nanozymes;Vegetable oils

期刊名称： Food Chemistry

ISSN： 0308-8146

年卷期： 2025 年 482 卷

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： Lateral flow immunoassays (LFIA) have been acknowledged as the promising on-site screening method for food survey. Monoclonal antibodies against benzo[a]pyrene (BaP) conjugated with enzyme-mimetic probes, a multi mode LIFA strips were systemically constructed and evaluated. The PBNPs@mAbs based LFIA could conduct triple measurement in 14 min. This assay had good limit of detections (LODs of 0.057, 0.035, and 0.106 μg kg−1), ultra calibration ranges (0.23–500, 0.076–56, 0.69–167 μg kg−1), and the recoveries ranging from 86 % to 105 % with the precision less than 12 %. Compared with the reported methods, the LODs were 175-, 285- and 94- folds enhanced, and dynamic linear ranges were greatly expanded. Finally, this novel PBNPs@mAbs LFIA assay with simplicity, rapidity, and sensitivity was successfully utilized for the determination of BaP in vegetable oils, which could be used as a superior enzyme-mimetic on-site analytic platform in food safety.

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