数字农科院2.0

Genomic Insights Into A Plant Growth-Promotingpseudomonas Koreensisstrain With Cyclic Lipopeptide-Mediated Antifungal Activity

文献类型： 外文期刊

作者： Gu, YL; Ma, YN; Wang, J; Xia, ZY; Wei, HL

作者机构：

关键词： Biological Control; Cyclic Lipopeptides; Non-Ribosomal Peptide Synthetase; Pseudomonas Koreensis; Secondary Metabolite

期刊名称： MICROBIOLOGYOPEN

ISSN： 2045-8827

年卷期： 2020 年 9 卷 9 期

页码：

收录情况： JCR（2021版）

摘要： Strain S150 was isolated from the tobacco rhizosphere as a plant growth-promoting rhizobacterium. It increased plant fresh weight significantly and lateral root development, and it antagonized plant pathogenic fungi but not phytobacteria. Further tests showed that strain S150 solubilized organic phosphate and produced ammonia, siderophore, protease, amylase, and cellulase, but it did not produce indole-3-acetic acid. Using morphology, physiological characteristics, and multi-locus sequence analysis, strain S150 was identified asPseudomonas koreensis. The complete genome of strain S150 was sequenced, and it showed a single circular chromosome of 6,304,843 bp with a 61.09% G + C content. The bacterial genome contained 5,454 predicted genes that occupied 87.7% of the genome. Venn diagrams of the identified orthologous clusters ofP. koreensisS150 with the other three sequencedP. koreensisstrains revealed up to 4,167 homologous gene clusters that were shared among them, and 21 orthologous clusters were only present in the genome of strain S150. Genome mining of the bacteriumP. koreensisS150 showed that the strain possessed 10 biosynthetic gene clusters for secondary metabolites, which included four clusters of non-ribosomal peptide synthetases (NRPSs) involved in the biosynthesis of cyclic lipopeptides (CLPs). One of the NRPSs possibly encoded lokisin, a cyclic lipopeptide produced by fluorescentPseudomonas. Genomic mutation of thelokAgene, which is one of the three structural NRPS genes for lokisin in strain S150, led to a deficiency in fungal antagonism that could be restored fully by gene complementation. The results suggested thatP. koreensisS150 is a novel plant growth-promoting agent with specific cyclic lipopeptides and contains a lokisin-encoding gene cluster that is dominant against plant fungal pathogens.

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