数字农科院2.0

Effect of high-moisture extrusion and addition of transglutaminase on major peanut allergens content extracted by three step sequential method

文献类型： 外文期刊

作者： Shah Faisal;Jinchuang Zhang;Shi Meng;Aimin Shi;Liu Li;Qiang Wang;Soheila J. Maleki;Benu Adhikari

作者机构：

关键词： High-moisture extrusion (HME) processing;Peanut allergen;Peanut-protein extrudate meat analogue;SDS-PAGE;Solubility;Western blot

期刊名称： Food Chemistry

ISSN： 0308-8146

年卷期： 2022 年 385 卷

页码：

收录情况： SCIE（2022版） ; EI（2022版）

摘要： The effect of high-moisture extrusion (HME) with or without transglutaminase (TGase) on peanut allergen levels and their extractability was studied. A three-stage sequential protein extraction significantly improved the protein recovery in processed samples (extrudate meat analogue); from 5.56 to 18.75 mg/100 mg without TGase, and from 4.59 to 20.82 mg/100 mg with 0.3% TGase. The total major allergen content was reduced by 91% (Ara h 1), 61% (Ara h 2), 60% (Ara h 6), and 55% (Ara h 3). Western-blot analysis of soluble extracts reflected the presence of Ara h 1 and Ara h 2 in significantly lower, indicating a potential reduction in IgE binding. During different processing zones, the most significant reduction in allergenic proteins was in the melting zone. The significant alteration in secondary and tertiary structures as a result of crosslinking shearing and degradation of proteins is likely to lead to allergen reduction.

分类号：

  • 相关文献

[1]苜蓿盲蝽气味结合蛋白Alin OBP3的克隆、表达及结合特性分析. 孙洋,任丽燕,张雪莹,张永军,吴孔明,郭予元. 2010

[2]重组猪戊型肝炎病毒ORF2区主要抗原域的原核表达及鉴定. 于婧,曲娟娟,于敏,赵立平,相文华. 2008

[3]牛分枝杆菌MPB83基因的原核表达及免疫活性分析. 张秀华,刘思国,王春来,郭设平,郭洋,邵美丽,宫强,彭永刚,沈国顺. 2005

[4]拟南芥光敏色素D(AtphyD)在不同光质下的表达特性分析. 李建平,杨建平,宋梅芳,苏亮,侯佩,郝晓燕,邵琳,陈果,足木热木. 2014

[5]日本血吸虫四跨膜孤儿受体(TOR)克隆及其第一个膜外区基因的表达. 马帅,韩艳辉,洪炀,张旻,曹晓丹,韩倩,刘艳涛,陆看,马茜茜,陆珂,贾秉光,林矫矫,傅志强. 2014

[6]苜蓿盲蝽气味结合蛋白AlinOBP3的克隆、表达及结合特性分析. 孙洋,任丽燕,张雪莹,张永军. 2010

[7]棉铃虫保幼激素环氧水解酶基因的克隆及其原核表达. 吴孔明,高希武,程红梅. 2009

[8]猪流感病毒新型核糖体移码蛋白PA-X特有序列的原核表达、纯化及鉴定. 宫晓倩,阮宝阳,刘晓敏,张鹏,汪琪,汪秀会,周艳君,李泽君,童武,郑浩,童光志,于海. 2016

[9]水牛卵母细胞成熟前后的差异蛋白质组的分析. 付强,刘振方,李敏玲,黄渝淋,梁贤威,张明. 2015

[10]活化蛋白激酶C受体1影响柔嫩艾美耳球虫子孢子入侵细胞的初步研究. 赵宗平,朱顺海,黄兵,韩红玉,赵其平,吕凌,陈婷,严茗,董辉. 2018

[11]一种新型木聚糖酶基因在毕赤酵母中的表达. 李琦,成莉凤,段盛文,冯湘沅,郑科,杨琦,刘志远,彭源德. 2018

[12]传染性法氏囊病病毒VP3抗原表位的鉴定. 邓小芸,高玉龙,高宏雷,祁小乐,王晓艳,王笑梅. 2007

[13]小麦TaPDK基因的序列分析、原核表达及纯化. 杨书玲,张龙雨,张改生,桑青,刘红占,朱启迪,张新钵,赵新亮. 2013

[14]黄瓜花叶病毒RNA3基因间隔区的功能研究. 杨磊,张建平,孙鑫,卢全有. 2020

[15]大豆(Glycine max(L.)Merr.)致敏蛋白Gly m Bd 30K检测方法及低致敏优异种质鉴定. 周明明,张明俊,王俊,邱丽娟. 2021

[16]灰飞虱VAP-B蛋白的抗体制备及体内外应用. 赵霞,秦发亮,刘文文,王锡锋. 2022

[17]Comparative proteome analysis of glutenin synthesis and accumulation in developing grains between superior and poor quality bread wheat cultivars. Ma, Wujun,Liu, Wan,Gao, Xuan,Wang, Ke,Wang, Shunli,Yan, Yueming,Zhang, Yanzhen,Zhang, Yong,He, Zhonghu,Zhang, Yong,He, Zhonghu. 2012

[18]Impact of vacuum mixing on protein composition and secondary structure of noodle dough. Zhang, Yingquan,Wu, Liang,Kong, Yan,Wei, Yimin,Liu, Rui,Sun, Junmao,Xing, Yanan.

[19]Allelic variation at the Glu-1 and Glu-3 loci, presence of the 1B.1R translocation, and their effects on mixographic properties in Chinese bread wheats. Liu, L,He, ZH,Yan, J,Zhang, Y,Xia, XC,Pena, RJ. 2005

[20]A MALDI-TOF based analysis of high molecular weight glutenin subunits for wheat breeding. Liu, Li,Wang, Aili,Ma, Junhong,Lan, Ping,Ma, Wujun,Liu, Li,Xia, Xianchun,He, Zhonghu,Wang, Aili,Yan, Yueming,He, Zhonghu,Bekes, Frank,Appels, Rudi. 2009

作者其他论文 更多>>