数字农科院2.0

Salivary Protein Cyclin-Dependent Kinase-like from Grain Aphid Sitobion avenae Suppresses Wheat Defense Response and Enhances Aphid Adaptation

文献类型： 外文期刊

作者： Yumeng Zhang;Xiaobei Liu;Yu Sun;Yong Liu;Yong Zhang;Tianbo Ding;Julian Chen

作者机构：

关键词： aphid adaptation;bacterial type III secretion system;defense response;salivary protein;Sitobion avenae

期刊名称： International Journal of Molecular Sciences

ISSN： 1661-6596

年卷期： 2024 年 25 卷 9 期

页码：

收录情况： SCIE（2024版）

摘要： Aphids are insect pests that suck phloem sap and introduce salivary proteins into plant tissues through saliva secretion. The effector of salivary proteins plays a key role in the modulation of host plant defense responses and enhancing aphid host adaptation. Based on previous transcriptome sequencing results, a candidate effector cyclin-dependent kinase-like (CDK) was identified from the grain aphid Sitobion avenae. In this study, the function of SaCDK in wheat defense response and the adaptation of S. avenae was investigated. Our results showed that the transient overexpression of SaCDK in tobacco Nicotiana benthamiana suppressed cell death triggered by mouse pro-apoptotic protein-BAX or Phytophthora infestans PAMP-INF1. SaCDK, delivered into wheat cells through a Pseudomonas fluorescens-mediated bacterial type III secretion system, suppressed callose deposition in wheat seedlings, and the overexpression of SaCDK in wheat significantly decreased the expression levels of salicylic acid and jasmonic acid signaling pathway-related genes phenylalanine ammonia lyase (PAL), pathogenesis-related 1 protein (PR1), lipoxygenase (LOX) and Ω-3 fatty acid desaturase (FAD). In addition, aphid bioassay results showed that the survival and fecundity of S. avenae were significantly increased while feeding on the wheat plants carrying SaCDK. Taken together, our findings demonstrate that the salivary protein SaCDK is involved in inhibiting host defense response and improving its host adaptation, which lays the foundation to uncover the mechanism of the interaction of cereal aphids and host plants.

分类号：

  • 相关文献

[1]Activation of Wheat Defense Response by Buchnera aphidicola-Derived Small Chaperone Protein GroES in Wheat Aphid Saliva. Qian Li,Yu Fu,Xiaobei Liu,Jingxuan Sun,Maolin Hou,Yong Zhang,Julian Chen. 2022

[2]Silencing an aphid-specific gene SmDSR33 for aphid control through plant-mediated RNAi in wheat. Zhang J.,Li H.,Zhong X.,Tian J.,Segers A.,Xia L.,Francis F.. 2023

[3]Salivary Effector Sm9723 of Grain Aphid Sitobion miscanthi Suppresses Plant Defense and Is Essential for Aphid Survival on Wheat. Yong Zhang,Xiaobei Liu,Yu Fu,Leonardo Crespo-Herrera,Huan Liu,Qian Wang,Yumeng Zhang,Julian Chen. 2022

[4]A Comprehensive Transcriptomic and Proteomics Analysis of Candidate Secretory Proteins in Rose Grain Aphid, Metopolophium dirhodum (Walker). Atsbha Gebreslasie Gebrekidan,Yong Zhang,Julian Chen. 2025

[5]AgoArmet and AgoC002: key effector proteins in cotton aphids host adaptation. Hui Xue,Mengjie Yan,Xiangzhen Zhu,Li Wang,Lizhen Chen,Junyu Luo,Jinjie Cui,Xueke Gao. 2024

[6]Influence of Garlic Intercropping or Active Emitted Volatiles in Releasers on Aphid and Related Beneficial in Wheat Fields in China. Chen Ju-lian,Sun Jing-rui,Cheng Deng-fa,Zhou Hai-bo,Francis, Frederic,Haubruge, Eric,Liu Yong,Bragard, Claude. 2013

[7]Climate warming may increase aphids' dropping probabilities in response to high temperatures. Ma, Chun-Sen.

[8]Variation in the transmission of barley yellow dwarf virus-PAV by different Sitobion avenae clones in China. Yu, Wenjuan,Cheng, Dengfa,Chen, Julian,Yu, Wenjuan,Francis, Frederic,Xu, Zhaohuan,Bragard, Claude,Liu, Yong. 2013

[9]Discovery of English grain aphid (Hemiptera: Aphididae) biotypes in China. Chen, Ju-Lian,Cheng, Deng-Fa,Sun, Jing-Rui,Xu, Zhao-Huan,Liu, Yong,Francis, Frederic.

[10]Detoxification of gramine by the cereal aphid Sitobion avenae. Cai, Qing-Nian,Han, Ying,Hu, Yuan,Zhao, Xin,Cao, Ya-Zhong.

[11]Vectoring ability of aphid clones of Rhopalosiphum padi (L) and Sitobion avenae (Fabr) and their capacity to retain barley yellow dwarf virus. Guo, JQ,Lapierre, H,Moreau, JP. 1997

[12]Impact of Wheat-Mung Bean Intercropping on English Grain Aphid (Hemiptera: Aphididae) Populations and Its Natural Enemy. Chen, Ju-Lian,Cheng, Deng-Fa,Zhou, Hai-Bo,Sun, Jing-Rui,Liu, Yong,Francis, Frederic. 2012

[13]Fluorescence competition assay for the assessment of green leaf volatiles and trans-beta-farnesene bound to three odorant-binding proteins in the wheat aphid Sitobion avenae (Fabricius). Yin, Jiao,Deng, Sisi,Li, Kebin,Cao, Yazhong.

[14]Evaluation of extraction procedures for 2-DE analysis of aphid proteins. An Shaoli,Wang Tao,Fang Kui,Zhang Hua,Sun Yu,Yang Xun,Xi Jinghui,Li Kebin.

[15]Effects of cycloxaprid, a novel cis-nitromethylene neonicotinoid insecticide, on the feeding behaviour of Sitobion avenae. Sun, Lina,Yang, Daibin,Yan, Xiaojing,Yuan, Huizhu.

[16]Adaptation of Wheat-Pea Intercropping Pattern in China to Reduce Sitobion avenae (Hemiptera: Aphididae) Occurrence by Promoting Natural Enemies. Chen, Julian,Cheng, Dengfa,Zhou, Haibo,Chen, Lin,Francis, Frederic,Haubruge, Eric,Liu, Yong,Bragard, Claude.

[17]Genetic Variation Among the Geographic Population of the Grain Aphid, Sitobion avenae (Hemiptera: Aphididae) in China Inferred from Mitochondrial COI Gene Sequence. Xu Zhao-huan,Chen Ju-lian,Cheng Deng-fa,Xu Zhao-huan,Liu Yong,Francis, Frederic. 2011

[18]Bacterial symbionts, Buchnera, and starvation on wing dimorphism in English grain aphid, Sitobion avenae (F.) (Homoptera: Aphididae). Li, Xiangrui,Zhang, Yunhui,Cheng, Dengfa,Coates, Brad,Zhou, Xuguo Joe. 2015

[19]Molecular Characterization And Gene Silencing O.f Laccase 1 In T he Grain Aphid, Sitobion Avenae. Zhang, Y,Fan, J,Francis, F,Chen, JL. 2018

[20]Sporadic Short Temperature Events Cannot B.e Neglected In Predicting I mpacts Of Climate Change On Small Insects. Ma, Chun-Sen,Zhu, Liang,Zhu, Liang,Wang, Lin. 2019

作者其他论文 更多>>